Product Description

Bingene Dye is a green fluorescent nucleic acid dye that is non-fluorescent by itself, but becomes fluorescent upon binding to dsDNA. It has the following features:

Lower fluorescent background.

Higher fluorescence intensity.

Less inhibitory than SYBR Green I and less likely to cause non-specific amplification.

Environmentally safe, non-mutagenic, and non-cytotoxic. Unsurpassed thermal stability, chemical stability, and photostability.

Compatible with instruments equipped with the 488 nm argon laser or any visible light excitation with wavelength in the region.

Amplification reaction can be monitored using existing optical setting for SYBR Green I or FAM on any commercial real-time PCR cycler.

Kit Contents

Component	Size	Packing
Bingene Dye, 20×	1 mL	1.5 mL Blue Cap Tube

Storage and Handling

Store the product at 2-25℃. The product is stable for at least one year from date of receipt.

Procedures

Set up the nucleic acid amplification reaction as usual, add 20×Bingene Dye at a ratio of 20:1 (for a reaction of 20mL final volume, add 1 mL Bingene Dye), add ddH2O to the final volume of choice.

Perform amplification on a thermocycling fluorometer and record the fluorescence signal at the annealing or extension step for PCR or collect signal in every minute for isothermal amplification.

Note: When using ABI Sequence Detection Systems, make sure to select NONE for the passive reference under the tab WELL INSPECTOR

Dye for Nucleic Acid Amplification-SYBR Green Alternat