Note：Four times the concentration of aminoacids and vitamins,low-glucose type (1000mg/L),without animal-derived components.

Product Description

Dulbecco's Modified Eagle's Medium (DMEM) is a modified Eagle Basal cell medium (BME) containing four times the concentration of amino acids and vitamins.This product is a low-glucose type (1000mg/L) and supports the cultivation of various mammalian cells, including primary fibroblasts, neurons, gliocytes, HUVECs and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7 and PC-12. This product is a GMP grade medium without animal-derived components. Therefore, additional culture additives such as fetal bovine serum (FBS) need to be supplemented when culturing cells.

Specifications

Volume	500mL
Supplementation	Need additional
Glutamine	L-Glutamine
Phenol Red	Phenol Red
Antibiotics	No Antibiotics
Sodium Pyruvate	Sodium Pyruvate
Sodium Bicarbonate	Sodium Bicarbonate
HEPES Buffer	No HEPES
pH	7.0-7.6
Osmolality	295~335 mOsmol/kg
Endotoxin	<0.3 EU/mL

Intended Use

The medium is produced by aseptic processes and can be used for further manufacturing purposes. This product is not intended for human, diagnostic or therapeutic use.

Formulation

Components (mg/L)

Amino Acids

Glycine	30
L-Arginine•HCl	84
L-Cystine•2HCl	63
L-Glutamine	584
L-Histidine•HCl•H 2 O	42
L-Isoleucine	105
L-Leucine	105
L-Lysine•HCl	146
L-Methionine	30
L-Phenylalanine	66
L-Serine	42
L-Threonine	95
L-Tryptophan	16
L-Tyrosine•2Na•2H 2 O	104
L-Valine	94

Vitamins

Choline chloride	4
Folic Acid	4
myo-Inositol	7.2
Niacinamide	4
D-Pantothenic Acid•1/2 Ca	4
Pyridoxal•HCl	4
Riboflavin	0.4
Thiamine•HCl	4

Inorganic Salts

CaCl₂	200
Fe(NO₃)₃•9H₂O	0.1
MgSO₄	97.67
KCl	400
NaHCO₃	3700
NaCl	6400
NaH₂PO₄	109

Other

D-Glucose	1000
Phenol Red•Na	15
Sodium Pyruvate	110

Storage

Stored at 2℃~8℃, away from light, the shelf life is 12 months.

Notes

Avoid repeated freezing and thawing.
Pay attention to operate in an aseptic condition.

Document

Dulbecco’s Modified Eagle’s Medium-Low Glucose-Biosci™

Dulbecco's Modified Eagle's Medium-Low Glucose-No HEPES - USDA