Description

Our dNTPs are analytically tested to ensure a purity of >99% by HPLC, guaranteeing optimal performance in demanding PCR applications.

Specification

Parameter	Specification
Purity	≥ 99% (HPLC)
Total Product Size	0.5 mL
Individual Container Size	0.5 mL
Number of Containers	1
Refrigeration Requirements	Freezer
Shipping Conditions	ICE
UNSPSC Code	12352208
UNSPSC Category	Nucleic Acid Bases
Grade	Biotech
DG	No
Storage	(-15 to -20)℃
Sterile	No
Stock	Usually in Stock

Summary

DNase, RNase: None detected.

Suitable for use in the Polymerase Chain Reaction (PCR).

PCR Suitability

dNTP Mix is a solution containing each of the four deoxynucleotides as follows:

10 mM dATP
10 mM dCTP
10 mM dGTP
10 mM dTTP

dNTP Mix was tested at a final concentration of 200 μM in a reaction mixture containing standard PCR components. A single band of approximately 500 base pairs was visualized following electrophoresis, confirming high performance.

Quality Control

Endonuclease-Exonuclease
One μg of λ Hind III fragments was incubated for 16 hours at 37 °C with dNTP Mix at a final concentration of 5 mM in a 50 μl reaction mixture containing 30 mM TrisHCl, pH 7.8, 50 mM NaCl and 10 mM MgCl2. No degradation of the DNA fragments was detected following agarose gel electrophoresis. Detection limit: Degradation of 10% of the DNA substrate is detectable.

Endonuclease (Nickase)
One μg of pBR322 DNA was incubated for 16 hours at 37 °C with dNTP Mix at a final concentration of 5 mM in a 50 μl reaction mixture containing 30 mM Tris-HCl, pH 7.8, 50 mM NaCl and 10 mM MgCl2. No conversion of the covalently closed circularDNA to the nicked or linear form was observed following agarose gel electrophoresis. Detection limit: Conversion of 1% of the DNA substrate is detectable.

RNase
Two μg of transfer RNA were incubated for 16 hours at 37 °C with dNTP Mix at a final concentration of 5 mM in a 50 μl reaction mixture containing 30 mM Tris-HCl, pH 7.8, 50 mM NaCl and 10 mM MgCl2. No degradation of the tRNA was detected following polyacrylamide gel electrophoresis. Detection limit: Degradation of 10% of the tRNA substrate is detectable.

Documents

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Disclaimer

For laboratory research use only.

dNTP mixture (10mM)