Description

The Crithidia fasciculata Probe qPCR Kit is designed for the qualitative detection of Crithidia fasciculata DNA using probe-based real-time PCR. Purified DNA is amplified with target-specific primers and a fluorescent probe for real-time monitoring.

The kit supports laboratory research involving cultured organisms, kinetoplast DNA, kinetoplastid biology, and insect–protozoan interactions.

Taxonomic note: Crithidia luciliae remains listed separately in some databases and culture collection records. Molecular research has associated the ATCC 30258 culture with Crithidia fasciculata. Detection coverage depends on the validated primer-probe design.

Product note: The standard kit is supplied without an internal control.

Specifications

Product Name	Crithidia fasciculata Probe qPCR Kit
Target Organism	Crithidia fasciculata Léger
Related Taxonomic Name	Crithidia luciliae (Strickland) Wallace and Clark
Target Material	Extracted DNA
Detection Method	Probe-based real-time PCR
Detection Type	Qualitative detection
Pack Size	50 reactions
Internal Control	Not included
NCBI Taxonomy ID	5656
Intended Use	Laboratory research use only

Features

Qualitative detection of Crithidia fasciculata DNA.
Target-specific probe-based qPCR format.
Suitable for purified research DNA samples.
Includes a non-infectious plasmid-based positive control.
Supplied for 50 reactions.
For laboratory research use only.

Kit Components

Component	Description	Size	Lid Color
2× Probe qPCR Master Mix	Optimized buffer and enzyme mixture	500 µL	Blue
Primer-Probe Powder	Lyophilized target-specific primer and probe set	50 reactions	Brown
Positive Control	Non-infectious plasmid-based control	0.5 mL	Yellow
Nuclease-Free Water	For reaction preparation and dilution	1 mL	Green

Applications

Qualitative detection of Crithidia fasciculata DNA.
Molecular confirmation of laboratory cultures.
Kinetoplast and trypanosomatid molecular biology research.
Insect–protozoan interaction studies.

Use extracted DNA of suitable purity. Applicable sample types depend on the validated extraction method and laboratory workflow.

Storage & Shipping

Store at −20°C.
Ship under dry-ice conditions.
Avoid repeated freeze–thaw cycles.
Protect fluorescent probe components from prolonged light exposure.

FAQ

The kit is designed for the qualitative detection of Crithidia fasciculata DNA using probe-based real-time PCR.

Molecular studies have associated the ATCC 30258 culture listed as Crithidia luciliae with Crithidia fasciculata. Detection coverage should be confirmed from the validated primer-probe design.

No. The standard kit is supplied without an internal control.

No. This product is intended strictly for laboratory research use.

For Research Use Only. Not for use in diagnostic procedures.

Crithidia fasciculata Probe qPCR Kit