Specification

Quality Level	ISO 9001
Sterility	Sterile
Form	Liquid
Feature	Ready-to-use for cells; 10ml for 1L Mycoplasma broth
Packaging	1 bottle
Bottle Filling Volume	100 mL
PH	7.4 (in H2O)
Storage Temp	2-8℃ for 6 months；-20℃/ -80℃ for 10 months
Suitability	Mycoplasma、cells

Application

General cell culture:

Supports the growth and maintenance of a variety of human and animal cell lines, including primary cells, fibroblasts, and tumor cell cultures.
Tissue and organ culture:

Due to its inclusion of nucleosides and coenzymes (such as NAD, NADP, and Coenzyme A), CMRL-1066 is suitable for short-term maintenance of mammalian tissue fragments or organ explants in vitro.
Low-serum or serum-free systems:

The presence of ascorbic acid, glutathione, cholesterol, and Tween 80 helps maintain membrane stability and cellular vitality under low-serum conditions. Hence, it is frequently used in the development of serum-free or chemically defined culture systems.
Host–pathogen co-culture studies:

As a nutritionally rich basal medium, CMRL-1066 is often employed for the co-culture of cell-dependent microorganisms such as Mycoplasma, Rickettsia, or certain viruses.
Cell recovery and pre-freezing maintenance:

With multiple antioxidant and metabolic support components (e.g., glucose, niacinamide, FAD, glutathione), it is also used for short-term recovery culture prior to cryopreservation or thawing.

Features

Enhanced Lipid Supplementation:

Incorporation of soluble lipid components improves cellular and mycoplasma metabolism, optimizes membrane composition, and enhances stress resistance—resulting in superior culture performance.
Stable Physiological Buffering:

Formulated with 20 mM HEPES, maintaining pH precisely at 7.4 to ensure consistent environmental stability throughout incubation and handling.
Serum Protein Enrichment:

Addition of defined serum proteins enhances culture stability and reinforces overall environmental consistency for reproducible results.
Simplified, Indicator-Free System:

Sodium bicarbonate and phenol red have been removed to reduce variability and eliminate potential optical or chemical interferences during sensitive assays.
Optimized Amino Acid Profile:

L-glutamine and L-cysteine are excluded to minimize degradation-related byproducts and maintain long-term formulation stability.
Light-Protective Packaging:

Supplied in amber bottles to preserve the integrity of light-sensitive components and ensure sustained biochemical activity during storage.

Inorganic Salts

Calcium chloride dihydrate

Magnesium sulphate anhydrous

Potassium chloride

Sodium acetate anhydrous

Sodium chloride

Sodium dihydrogen phosphate anhydrous

Amino Acids

Glycine

L-Alanine

L-Arginine

L-Aspartic acid

L-Cystine

L-Glutamic acid

L-Histidine hydrochloride monohydrate

L-Isoleucine

L-Leucine

L-Lysine hydrochloride

L-Methionine

L-Phenylalanine

L-Proline

L-Serine

L-Threonine

L-Tryptophan

L-Tyrosine disodium salt dihydrate

L-Valine

Trans-4-Hydroxy-L-Proline

Vitamins & Cofactors

Choline chloride

D-Biotin

D-Pantothenate (hemicalcium)

Folic acid

L-Ascorbic acid sodium salt

Nicotinamide

Nicotinic acid

Pyridoxal hydrochloride

Pyridoxine hydrochloride

Riboflavin

Thiamine hydrochloride

myo-Inositol

p-Amino benzoic acid (PABA)

Nucleosides & Derivatives

2′-Deoxyadenosine

2′-Deoxycytidine hydrochloride

2′-Deoxyguanosine

5-Methyldeoxycytidine

Cholesterol

Cocarboxylase

Coenzyme A sodium salt

D-Glucose

D-Glucuronic acid sodium salt

FAD disodium salt

Glutathione (reduced)

Thymidine

Uridine-5-Triphosphate sodium salt

β-NAD

β-NADP sodium salt
Lecithin

Documents

M-CMRL-10 SDS

CMRL 1066(10X)