Description

The Human Urine cfDNA Extraction Kit (Cat. CF-DNA-301) is designed for high-efficiency isolation of circulating cell-free DNA (cfDNA) from up to 50 mL of urine in a single preparation. The kit utilizes an innovative double-layer column technology that maximizes cfDNA recovery while eliminating genomic DNA contamination from lysed white blood cells.

The eluted cfDNA is highly pure and immediately ready for downstream molecular applications, including next-generation sequencing (NGS), qPCR, digital PCR, and methylation analysis.

This kit is particularly suited for applications requiring large input volumes, such as early-stage cancer detection or liquid biopsy studies where cfDNA abundance is low.

Performance Specifications

Parameter	Specification
Sample volume	Up to 50 mL urine
cfDNA fragment size	80–500 bp
Recovery rate	≥90%
Yield	>20 μg total cfDNA
Purity	Direct-use for NGS / PCR
Genomic DNA contamination	None detectable (WBC gDNA retained on membrane)
Format	50 Preps

Applications

This high-capacity urine cfDNA extraction kit is optimized for:

Early cancer detection & minimal residual disease (MRD)
(large input volume improves sensitivity for low-abundance markers)
Liquid biopsy & non-invasive genomic profiling
Urothelial carcinoma screening
Prenatal screening (urine-based cfDNA research)
Transplant rejection monitoring
Infectious disease monitoring via urinary cfDNA
Epigenetic and methylation assays
Biomarker discovery from low-input urine samples

Large-volume capability significantly enhances assay sensitivity, making the CF-DNA-301 especially suitable for early-stage or low-abundance cfDNA studies where typical 1–5 mL plasma inputs are insufficient.

Sample Volumes

Kit Variant	Sample Volume
#301	Up to 50ml of Urine
#302	Up to 5ml of Plasma
#303	Up to 0.7ml of Plasma

Storage

Store at RT (20-25C).

Related Products

(Click Here) #302 cf-DNA Extraction Kit

(Click Here) #303 cf-DNA Extraction Kit

Product Manual

#301 User Manual

#302 User Manual

#303 User Manual

This product is intended for research use only.

Human Urine cfDNA Extraction Kit (50 Preps, 50 mL Input Capacity)