Name: Carboxypeptidase B from Porcine Pancreas
SKU: BF-1210200
Price: 3.00 USD

Question 1

What is Carboxypeptidase B?

Accepted Answer

Carboxypeptidase B (EC 3.4.12.3) is a zinc-containing pancreatic exopeptidase that selectively hydrolyzes peptide bonds at the C-terminal basic amino acids lysine, arginine and ornithine. It is also known as protaminase or peptidyl-L-lysine [L-arginine] hydrolase, with a molecular weight of 34,300 Da.

Question 2

What is the source and form of this product?

Accepted Answer

This Carboxypeptidase B is derived from porcine pancreas and supplied as a lyophilized powder.

Question 3

How should Carboxypeptidase B lyophilized powder be stored and reconstituted?

Accepted Answer

Store the lyophilized powder at -20°C. Reconstitute by dissolving in distilled water or a dilute buffer.

Question 4

What is the specific activity and protein content of this Carboxypeptidase B?

Accepted Answer

The specific activity is approximately 50 U/mg protein, with a protein content of approximately 80% as determined by the biuret method.

Question 5

How is Carboxypeptidase B activity defined?

Accepted Answer

One unit of Carboxypeptidase B hydrolyzes 1 μmol of hippuryl-L-arginine per minute at 25°C and pH 7.65.

Question 6

How is Carboxypeptidase B activity measured?

Accepted Answer

Activity is measured by the increase in absorbance at 254 nm resulting from the hydrolysis of hippuryl-L-arginine. The initial linear rate (ΔA254/min) is used with the hippuric acid extinction coefficient of 0.349 to calculate units per mg enzyme.

Question 7

Which amino acids does Carboxypeptidase B cleave?

Accepted Answer

Carboxypeptidase B selectively hydrolyzes the C-terminal basic amino acids lysine, arginine and ornithine. Example reaction: Peptidyl-L-lysine + H2O → Peptide + L-Lysine.

Question 8

Is Carboxypeptidase B a metalloenzyme?

Accepted Answer

Yes. Carboxypeptidase B is a zinc-containing metalloenzyme (metallocarboxypeptidase); one atom of zinc is required for catalytic activity.

Product Name	Carboxypeptidase B
Synonyms	Protaminase; Peptidyl-L-lysine [L-arginine] hydrolase
Catalog Number	BF-1210200
EC Number	3.4.12.3
Biological Source	Porcine pancreas
Form	Lyophilized powder
Specific Activity	Approximately 50 U/mg protein
Protein Content	Approximately 80% (biuret method)
Molecular Weight	34,300 Da
Solubility	Distilled water or dilute buffer
Storage	-20°C
Unit Definition	One unit hydrolyzes 1 µmol hippuryl-L-arginine per minute at 25°C, pH 7.65
Assay Method	Increase in absorbance at 254 nm during hydrolysis of hippuryl-L-arginine
Reference Price	$3.00 / mg

Description

Specification

Features

Application

FAQ