Description

CAPS is a zwitterionic buffering agent used in biochemistry and molecular biology. It is a cyclohexylamine buffer that is useful for a pH range of 9.7 – 11.1 and commonly used to study enzymatic processes above physiological pH. CAPS is an excellent buffer for the transfer of high molecular weight proteins to nitrocellulose membranes after SDS-PAGE. It is also used in protein sequencing. CAPS displays only weak or negligible complexation capabilities for some metals making it a suitable non-coordinating buffer for use in solutions containing metal ions. CAPS is suitable for use with the bicinchoninic acid (BCA) assay.

Specifications

Item	Value
Chemical Name	3-(Cyclohexylamino)-1-Propanesulfinic Acid
Appearance	White Crystal
CAS No.	1135-40-6
Molecular Formula	C9H19NO3S
Molecular Weight	221.3
Grade	Biochemical Grade
Purity	≥99.0%
Water Solubility	Colorless, Clear, Transparent
Heavy Metals	≤3ppm
Moisture	≤0.5%
pH (1% Aqueous Solution at 25℃)	4.8-6.0
UV Absorbance at 260nm	≤0.020abs unit
UV Absorbance at 280nm	≤0.038abs unit
Storage / Handling	Store at Room Temperature.

Features

High-Purity Grade: ≥ 99.0% biochemical grade ensures minimal impurities, suitable for protein sequencing and sensitive assays.
Optimal Alkaline Buffering Range: Effective buffering between pH 9.7–11.1, supporting enzymatic reactions and protein analyses.
Low UV Absorbance & Low Interference: Minimal absorbance at 260 nm / 280 nm reduces background interference in nucleic acid or protein assays.
Non-Coordinating with Metal Ions: Weak or negligible complexation with metal ions, preserving enzyme or protein activity.
Excellent Solubility and Stability: Water-soluble, clear solution. Solid powder stable at room temperature and easy to handle.

Applications

Protein electrophoresis & Western blot transfers: Suitable for SDS-PAGE and transfer of high molecular weight proteins to nitrocellulose or PVDF membranes.
Protein sequencing & sample preparation: Ideal for N-terminal protein sequencing without buffer interference.
Enzymatic assays under alkaline conditions: Supports enzyme kinetics and assays requiring pH above physiological range.
Protein crystallization, purification, or chromatography: Suitable for crystallization trials, chromatography, and protein purification workflows.
Protein quantification assays: Compatible with Bicinchoninic Acid (BCA) Assay for accurate protein concentration measurement.

Safety Disclaimer

This product is intended for laboratory research use only. Not for drug, human, or veterinary use. Review the Safety Data Sheet (SDS) provided by BioFargo for detailed handling, storage, hazard, and disposal information. Use appropriate personal protective equipment (PPE) including gloves, lab coat, and eye protection when handling. Dispose of waste and residual buffer in accordance with local, state, and federal regulations. Ensure proper ventilation if preparing large volumes or heating solutions.

Documents

INFO

COA

SDS

CAPS Free Acid 99.0% Biochemical Grade