Description

Can Get Signal™ immunostain is a reaction solution that contains an accelerator for antigen-antibody reactions, which improves sensitivity, specificity, and S/N of immunohistochemistry (IHC) and immunocytochemistry.

Features

• Improves sensitivity, specificity, and S/N of IHC.
• Can be applied to various detection systems (e.g., chromogenic, chemiluminescence, or fluorescence).
• Can be used with ABC or polymer complex methods.
• Solutions A and B exhibit various properties for improving results.
• Can be used directly without dilution (Ready-to-use).

Application

・Immunohistochemistry (IHC)
・Immunocytochemistry

Specification

Category	Item	Details
Storage condition	-	Store at 4ºC

Components

This kit includes the following components. All reagents should be stored at 4ºC and protected from light.

Reagent Name	Code No.
Reagent Name	NKB-401	NKB-501	NKB-601
Solution A	5 mL	20 mL	-
Solution B	5 mL	-	20 mL

Typical reaction flow

Flow chart of immunostaining with Can Get Signal™ immunostain

Application Data

Example 1.Detection of PCNA using paraffin-embedded sections

The localization of PCNA (proliferating cell nuclear antigen) expression in human skin was detected using paraffin-embedded sections of the human skin tissue model TESTSKINTM (Toyobo). Detection was performed by the ABC method with anti-PCNA mouse monoclonal antibody as the primary antibody and biotinylated mouse IgG as the secondary antibody. Each antibody was diluted with Solution A of Can Get Signal™ immunostain prior to use. As a control experiment, PBS(-) containing 1.5% normal horse serum (conventional method) was used instead of Solution A of Can Get Signal™ immunostain. As a result, Can Get Signal™ immunostain produced higher signals and lower background than the conventional method.

Fig. 1. IHC detection of PCNA using paraffin-embedded tissue sections

Example 2. Detection of paxillin with the fluorescent antibody method

Fig. 2. Immunocytochemistry detection of paxillin with the fluorescent antibody method
*The data was kindly provided by Dr. Harada, Tokyo Institute of Technology.

Documents

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Can Get Signal Immunostain-Toyobo