Suggested Medium

M6621 Complete Epithelial Cell Medium w/ Kit – 500 ml

Product Description

C57BL/6 Mouse Primary Liver Epithelial Cells are isolated from the liver tissues of pathogen-free laboratory mice and grown in gelatin pre-coated tissue culture flask with Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10⁶ cells per ml and is delivered frozen. C57BL/6 Mouse Primary Liver Epithelial Cells are characterized by immunofluorescent staining with E-cadherin (Catalog 610182, BD) or ZO-1 (Catalog 617300, Life Technology) antibody. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.

Storage

Cryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.

Disclaimer

For Research Use Only

Investigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.