Description

Bluo-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Bluo-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Specifications

Grade	High Purity
Formula	C14H16BrNO6
MW	374.2
CAS	97753-82-7
Appearance	White to Off-white Powder
Color of Solution (4% in DMF)	Colorless
Turbidity (0.4% in DMF)	≤2.0 NTU
Water Content (K. Fischer)	≤0.5%
Purity(HPLC)	≥99.0 area-%
TLC (50mg in 1ml DMF and 9ml propanol; butanol/water 86:14;UV 254nm, sulfuric acid)	Corresponds to reference material
Specific Optical Rotation [alpha]^D (c=1 in DMF/water 1:1; 20o C)	-35.0 to -32.0
FT-IR Spectrum	Corresponds to reference spectrum
Storage	-15°C- -20°C under Nitrogen in dark

Application

Bluo-Gal is suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. Bluo-Gal is suitable for use as β-galactosidase substrate in the staining of skin tissues for electron microscopy analysis, Dictyostelium samples and in the staining of vena cava of adult mice.

Chromogenic substrate suitable for identification of lac+ bacterial colonies. An alternative to 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-Gal), producing a darker blue color.

Documents

COA

SDS

Disclaimer: For laboratory research use only.