Description

Biofargo Binding & Wash Buffer for Ni-NTA / Ni-IDA is a ready-to-use, precisely formulated buffer designed for His-tag protein purification by immobilized metal affinity chromatography (IMAC). Containing 10 mM imidazole at pH 7.9–8.1, this buffer effectively reduces non-specific protein binding while maintaining strong and selective interaction between His-tagged proteins and Ni-NTA or Ni-IDA resins.

Optimized for reliability and reproducibility, this buffer is fully compatible with gravity flow columns, spin columns, syringe formats, and FPLC systems, making it ideal for routine laboratory purification, method development, and scale-up workflows.

Specifications

Parameter	Description
Buffer Type	Binding / Wash Buffer
Imidazole	10 mM
pH	7.9 – 8.1
Compatibility	Ni-NTA, Ni-IDA resins
Application	His-tag protein purification (IMAC)
Format	Ready-to-use solution
Storage	As recommended on label

Features

Optimized 10 mM Imidazole Concentration
Minimizes non-specific binding without disrupting His-tag–metal affinity interactions.
Controlled pH 7.9–8.1
Ensures optimal binding efficiency and protein stability during IMAC purification.
Compatible with Ni-NTA & Ni-IDA Resins
Suitable for all standard nickel-based affinity chromatography matrices.
High Ionic Strength Formulation
Reduces background proteins and improves purification selectivity.
Ready-to-Use Format
Eliminates buffer preparation time and reduces variability between experiments.
Reproducible & Scalable
Suitable for research, process development, and preparative purification workflows.

Applications

This buffer is designed for use in His-tag affinity purification workflows, including:

Binding and wash steps in Ni-NTA / Ni-IDA IMAC purification
Removal of weakly or non-specifically bound host proteins
Recombinant protein purification from bacterial, yeast, or mammalian lysates
Gravity flow columns, spin columns, and automated FPLC systems
Protein expression screening and downstream process optimization

Typical Workflow Compatibility

Column equilibration
Sample loading
Binding / Wash with 10 mM Imidazole Buffer
Elution with higher imidazole concentration buffer
This product is specifically optimized for Step 3, ensuring cleaner elution profiles and improved downstream purity.

Usage Instructions

Compatible with equilibration buffers and wash buffers for Ni-NTA/IDA resin purification. The imidazole concentration in the wash buffer depends on the protein sample. For most contaminating proteins, a concentration of 10–20 mM imidazole is typically recommended. For Ni-TED resin, use with product C600500.

Components

Components include: 10 mM Imidazole, NaCl, NaH2PO4·2H2O, pH 7.9~8.1

Why Choose Biofargo?

Biofargo provides high-quality, application-validated buffers designed to meet the needs of modern protein purification workflows.

Each formulation is developed with a focus on performance consistency, practical usability, and cost efficiency, making it a dependable alternative to premium brand buffers—without compromising results.

Binding/Wash Buffer For Ni-NTA/Ni-IDA,10 mM Imidazole pH 7.9~8.1