Description

Establishing an experimental anti-GBM nephritis model requires an antiserum that targets the rat glomerular basement membrane and provides sufficient material for a defined number of animals. Biofargo Bf-001AGBM is a sheep anti-rat isolated glomerular basement membrane serum supplied for the induction of passive anti-GBM nephritis in rats.

This nephrotoxic anti-GBM serum is supplied as 25 mL in 0.02 M phosphate-buffered saline at pH 7.3. One bottle contains sufficient antibody to induce passive anti-GBM nephritis in at least 25 rats weighing 175–200 g when used according to the stated directions.

The rat anti-GBM nephritis model is associated with mesangial proliferation, inflammatory cell vasculitis, glomerulosclerosis and renal fibrosis. The disease response is dose-dependent, so the complete dose must be delivered. Because antiserum lots, rat sources and experimental requirements can vary, a dose-response study should be performed to establish the desired disease severity for each lot.

Bf-001AGBM replaces the previous sheep anti-whole-rat-glomeruli serum. Both products target rat GBM and produce the same type of disease model. The current product has not been validated or recommended for use in mice.

Specification

Biofargo Bf-001AGBM product specifications
Parameter	Specification
Brand	Biofargo
Catalog number	Bf-001AGBM
Product name	Sheep Anti-Rat Isolated Glomerular Basement Membrane Serum
Alternative name	Sheep Anti-Rat Anti-GBM Serum
Product type	Nephrotoxic antiserum
Host species	Sheep
Target species	Rat
Target	Isolated rat glomerular basement membrane
Research model	Passive anti-GBM nephritis and immune-mediated glomerulonephritis
Form	Serum
Volume	25 mL
Buffer	0.02 M phosphate-buffered saline
pH	7.3
Heat treatment	56°C for 30 minutes
Storage	-20°C or below
Freeze-thaw instruction	Avoid repeated freeze-thaw cycles
Animal coverage	Sufficient to induce disease in at least 25 rats weighing 175–200 g when used according to the stated directions
Recommended rat strain	Sprague-Dawley
Mouse validation	Not validated or recommended for mice
Bulk quantities	Available

Features

Defined anti-GBM target: Sheep antiserum directed against isolated rat glomerular basement membrane.
Passive anti-GBM nephritis model: Supplied for the induction of immune-mediated glomerulonephritis in rats.
25 mL package: One bottle contains sufficient antibody for at least 25 rats weighing 175–200 g when used according to the stated directions.
Specified formulation: Supplied in 0.02 M phosphate-buffered saline at pH 7.3.
Heat-treated serum: Treated at 56°C for 30 minutes.
Frozen storage: Store at -20°C or below and avoid repeated freeze-thaw cycles.
Model-associated findings: The product page identifies mesangial proliferation, inflammatory cell vasculitis, glomerulosclerosis and renal fibrosis.
Bulk supply: Bulk quantities are available.

Application

Biofargo Bf-001AGBM sheep anti-rat GBM serum is supplied for the induction of passive anti-GBM nephritis and immune-mediated glomerulonephritis in rats.

Passive anti-GBM nephritis induction
Immune-mediated glomerulonephritis model establishment
Mesangial proliferation research
Inflammatory cell vasculitis research
Glomerulosclerosis research
Renal fibrosis research

Model Characteristics Described in the Product Data

Following administration of the anti-GBM serum, linear deposits of heterologous sheep IgG can become detectable in the glomerular basement membrane by immunofluorescence within minutes and increase in intensity during the following 3–5 days. Rat C3 can also localize in a linear pattern, and proteinuria can develop after 24 hours.

The autologous phase can become noticeable 8–10 days after anti-GBM antibody administration, with increased proteinuria and linear glomerular localization of rat IgG. By three weeks, the product data state that proteinuria can exceed 200 mg per 24 hours and that glomerular lesions can become apparent and increase in severity over time, leading to glomerulosclerosis.

FAQ

What is Biofargo Bf-001AGBM?

Bf-001AGBM is a sheep anti-rat isolated glomerular basement membrane serum supplied for the induction of passive anti-GBM nephritis and immune-mediated glomerulonephritis in rats.

What is nephrotoxic anti-GBM serum used for?

This nephrotoxic antiserum is used to induce an experimental rat anti-GBM nephritis model. The product information associates the model with mesangial proliferation, inflammatory cell vasculitis, glomerulosclerosis and renal fibrosis.

What species is Bf-001AGBM produced in?

The antiserum is produced in sheep and targets isolated rat glomerular basement membrane.

What is the package size?

Bf-001AGBM is supplied in a 25 mL bottle.

How many rats can be studied with one bottle?

One 25 mL bottle contains sufficient antibody to induce passive anti-GBM nephritis in at least 25 rats weighing 175–200 g when used according to the stated directions.

What rat strain is recommended?

The product data recommend the Sprague-Dawley rat strain. Other rat strains were not tested in the referenced product data.

What is the recommended dose of sheep anti-rat GBM serum?

The stated recommended dose is 0.4–0.5 mL per 100 g of body weight. Disease production is dose-dependent, and the complete dose must be delivered.

Should a dose-response study be performed?

Yes. Because antiserum lots, rat sources and investigator requirements can vary, the product data recommend performing a dose-response study to establish the desired disease severity for each lot.

What buffer is used for Bf-001AGBM?

The serum is supplied in 0.02 M phosphate-buffered saline at pH 7.3.

Has the serum been heat-treated?

Yes. The product has been heat-treated at 56°C for 30 minutes.

How should Bf-001AGBM be stored?

Store the sheep anti-rat GBM serum at -20°C or below and avoid repeated freeze-thaw cycles.

What should be done if a precipitate appears after thawing?

A slight precipitate may form after thawing. The product data instruct users to centrifuge the serum at 3,000–5,000 rpm for 30 minutes before injection.

Can Bf-001AGBM be used in mice?

The product has not been validated or recommended for use in mice.

What happened to the previous sheep anti-whole-rat-glomeruli serum?

Bf-001AGBM replaces the previous sheep anti-whole-rat-glomeruli serum. Both products target rat glomerular basement membrane and produce the same type of disease model.

Has chronic disease been verified with this anti-GBM serum?

Chronic disease was not verified in the referenced product data.

Are bulk quantities of Bf-001AGBM available?

Yes. Bulk quantities are available.

Bf-001AGBM Sheep Anti-Rat Isolated GBM Serum