Description

BCA Protein Quantification Kit is a sensitive and reliable protein assay kit designed for accurate total protein concentration determination in biological samples. During protein research workflows, inconsistent protein input amounts can affect downstream experiments such as Western blot, SDS-PAGE, immunoprecipitation, and biochemical analysis. Reliable protein quantification is therefore essential for sample normalization and experimental reproducibility.

This BCA Protein Assay Kit utilizes the bicinchoninic acid (BCA) colorimetric assay method to measure protein concentration through copper ion reduction and BCA-Cu+ complex formation. The generated purple color signal is proportional to protein concentration and can be quantified by measuring absorbance at 562 nm using a microplate reader or spectrophotometer.

With a broad detection range, high sensitivity, and excellent compatibility with common detergents, this protein quantification kit provides a convenient solution for cell lysates, tissue extracts, and purified protein samples. The kit is suitable for routine protein concentration measurement before Western blot, SDS-PAGE, and other protein analysis workflows.

Specifications

Product Name	BCA Protein Quantification Kit
SKU	AP0011M
Size	500 Tests
Product Type	BCA Protein Assay Kit
Detection Method	Colorimetric Protein Assay
Detection Wavelength	562 nm
Detection Range	25–1000 μg/mL
Minimum Detection Amount	0.5 μg Protein
Compatible Samples	Cell lysates, tissue extracts, purified proteins, biological samples
Storage	Room Temperature Shipping, Store Protected from Light
Shelf Life	2 Years

Features

Accurate total protein quantification: Provides reliable measurement of protein concentration using the widely used BCA assay method.
High sensitivity detection: Detects protein concentrations down to 25 μg/mL with a minimum detection amount of 0.5 μg protein.
Broad sample compatibility: Suitable for cell lysates, tissue extracts, purified proteins, and common biological samples.
Detergent compatible protein assay: Compatible with commonly used detergents including SDS, Triton X-100, and Tween series at appropriate concentrations.
Convenient standard curve preparation: Includes ready-to-use protein standard solution for simplified BCA protein assay workflows.
Microplate-based protein detection: Supports high-throughput protein quantification using 96-well plates and microplate readers.

Applications

BCA Protein Quantification Kit is widely used for protein concentration measurement in molecular biology, cell biology, and biochemical research applications.

Western Blot Sample Normalization: Determine protein concentration before SDS-PAGE loading to ensure consistent protein input between samples.
Cell Lysate Protein Quantification: Measure total protein concentration from cultured cell extracts prepared with common lysis buffers.
Tissue Protein Extraction Analysis: Quantify protein content from animal tissue lysates and complex biological samples.
SDS-PAGE Sample Preparation: Normalize protein amounts before electrophoresis for accurate protein comparison.
Immunoprecipitation Sample Preparation: Determine protein concentration before antibody-based enrichment workflows.
Protein Purification Analysis: Monitor protein recovery and concentration during purification procedures.
Recombinant Protein Quantification: Measure purified protein samples for downstream biochemical experiments.
Biochemical Research: Suitable for general protein concentration determination in laboratory research workflows.

Protocol Overview

The BCA Protein Quantification Kit provides a simple colorimetric workflow for protein concentration measurement.

Prepare protein standards using the supplied protein standard solution.
Add protein samples and standards into a 96-well plate.
Prepare BCA working reagent by mixing BCA Reagent A and BCA Reagent B at a 50:1 ratio.
Add BCA working reagent and incubate samples at 37°C for approximately 30 minutes.
Measure absorbance at 562 nm and calculate protein concentration using the standard curve.

For accurate protein concentration determination, it is recommended to generate a standard curve for each experiment because BCA color development can be affected by incubation time and temperature.

Ordering Information

SKU	Product	Size
AP0011M	BCA Protein Quantification Kit	500 Tests

FAQ

What is a BCA Protein Assay Kit used for?

A BCA Protein Assay Kit is used to determine total protein concentration in biological samples. It is commonly used before Western blot, SDS-PAGE, immunoprecipitation, and protein purification workflows for sample normalization.

How does the BCA protein quantification method work?

The BCA method is based on protein-mediated reduction of copper ions followed by formation of a purple BCA-Cu+ complex. The color intensity is proportional to protein concentration and is measured at 562 nm.

What samples are compatible with this protein quantification kit?

This BCA Protein Quantification Kit is suitable for cell lysates, tissue extracts, purified proteins, and many biological samples requiring total protein concentration measurement.

Can this BCA assay be used with RIPA lysates?

Yes. The BCA assay is commonly used for protein concentration measurement after RIPA protein extraction. However, samples containing strong reducing agents or high concentrations of chelators should be evaluated because they may interfere with the assay.

What substances can interfere with BCA protein assays?

High concentrations of reducing agents such as DTT and β-mercaptoethanol, as well as chelating agents such as EDTA and EGTA, may affect BCA assay performance. EDTA should generally remain below 10 mM, DTT below 1 mM, and β-mercaptoethanol below 0.01%.

Why is 562 nm used for BCA protein detection?

The BCA protein assay generates a purple color complex with maximum absorbance near 562 nm, allowing accurate protein concentration measurement using a microplate reader or spectrophotometer.

What is the difference between BCA and Bradford protein assays?

BCA assays generally provide better compatibility with many detergents and are widely used for cell lysate and tissue protein quantification, while Bradford assays may be more sensitive to detergent interference.