Background

This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product.

Description

The APC Mouse Monoclonal Antibody (ARA727) is a fluorochrome-conjugated primary antibody specifically designed for flow cytometric detection of mouse CD127 (Interleukin-7 Receptor alpha, IL-7Rα).
This antibody is directly conjugated to Allophycocyanin (APC), enabling high-sensitivity, single-step staining without the need for secondary antibodies.

Clone A7R34 recognizes an extracellular epitope of mouse CD127, a key immunological marker involved in T cell and B cell development, immune homeostasis, and lymphocyte differentiation.
The APC fluorophore provides bright fluorescence, low background, and excellent signal-to-noise performance, making this antibody ideal for multicolor flow cytometry panels.

Each lot is quality controlled for specificity and performance in Flow Cytometry (FC/FCM) and supplied with complete documentation.

For Research Use Only (RUO). Not for diagnostic or therapeutic use.

Specifications

Reactivity	Human, Mouse, Rat
Application	WB, IF/ICC, IP
Host	Mouse
Clonality	Monoclonal
Description	Rabbit monoclonal antibody to APC
Immunogen	A synthetic peptide of human APC
Purification Method	The antibody was purified by immunogen affinity chromatography.
Clonality	Monoclonal
Form	Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.
Gene Name	APC
Alternative Names	DP2.5; Adenomatous polyposis coli protein; Protein APC; Deleted in polyposis 2.5
Human Gene ID	324
Rat Gene ID	24205
Human Protein ID	P25054
Mouse Protein ID	Q61315
Rat Protein ID	P70478

Features

Primary Antibody, Directly Conjugated

APC-labeled monoclonal antibody enables direct staining, eliminating the need for secondary antibodies.
Highly Specific for Mouse CD127 (IL-7Rα)

Clone A7R34 specifically binds Interleukin-7 Receptor alpha, a well-established immune cell surface marker.
Optimized for Flow Cytometry (FC / FCM)

Validated for flow cytometric immunophenotyping of mouse immune cells.
Bright APC Fluorophore

Allophycocyanin offers strong fluorescence emission, ideal for low-abundance antigen detection and multicolor panels.
Reproducible & Reliable

Produced under strict quality control to ensure lot-to-lot consistency.
Complete Documentation Provided

Supplied with datasheet and relevant product information to support experimental reproducibility.

Application

Application	Dilution Ratio
WB	1:500 - 1:1000
IF/ICC	1:50 - 1:100
IP	1:10 - 1:50

Target Antigen: Mouse CD127 / IL-7Rα (Interleukin-7 Receptor alpha)
Clone: A7R34
Antibody Type: Monoclonal Primary Antibody
Host Species: Mouse
Conjugate: APC (Allophycocyanin)
Applications: Flow Cytometry (FC / FCM)
Species Reactivity: Mouse
Recommended Storage: 2–8 °C, protect from light
Regulatory Status: Research Use Only (RUO)

Data

Western blot analysis of APC expression in Hela (A), A549 (B), HL60 (C), U2OS (D) whole cell lysates. (Predicted band size: 312 kD; Observed band size: 160 kD)

Immunofluorescent analysis of APC staining in Lncap cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

APC Mouse Monoclonal Antibody(ARA727)