Description

Alizarin Red S, an anthraquinone derivative, may be used to identify calcium in tissue sections. The reaction is not strictly specific for calcium, since magnesium, manganese, barium, strontium, and iron may interfere, but these elements usually do not occur in sufficient concentration to interfere with the staining. Calcium forms an Alizarin Red S-calcium complex in a chelation process, and the end product is birefringent.

Specifications

Grade	Indicator
Formula	C14H7O7SNa
MW	342.26
CAS	130-22-3
Appearance	Yellow-green to orange-brown powder
Infrared spectrum	Conforms to Structure
Absorpt. Ratio P-15/P+15	0.97-1.07
Lambda max	554-558nm
Lambda max	590-599nm
Purity(HPLC)	Report
Storage	R.T

Features

Reliable Calcium Detection: Facilitates robust visualization of calcium deposits in tissue sections and cultured cells by forming a distinct chelation complex.
Research-Grade Quality: High-purity indicator suitable for histology, cytology, and biochemical applications.
Distinct Color Profile: Produces orange-red staining with birefringent complexes that enhance microscopic contrast.
Wide Research Utility: Commonly used in studies of osteogenic differentiation, bone biology, calcification, and mineralization.
Convenient Powder Form: Stable powder for flexible solution preparation at optimal pH ranges (often around pH 4.1–4.3) in staining workflows.

Applications

Histological Calcium Staining: Detects calcium deposits in tissue sections for morphological analysis.
Cell Culture Mineralization Assays: Used to assess osteogenic differentiation and bone formation in vitro.
Quantitative and Qualitative Analysis: Suitable for both visual inspection under microscopy and colorimetric quantification in research protocols.
Bone and Cartilage Research: Evaluates mineralization patterns in developmental biology, skeletal studies, and regenerative medicine models.
Educational and Analytical Use: Valuable for teaching labs and analytical demonstrations involving calcium complex formation.

Safety Disclaimer

Disclaimer: For laboratory research use only.

Documents

INFO COA SDS

Alizarin Red S Indicator Grade