Description

2xYT Broth is a liquid nutrient medium optimized for the high-density growth of E. coli and the efficient propagation of filamentous bacteriophages such as M13. This medium is an enhanced version of traditional YT, providing a richer formulation that supports vigorous bacterial cultures essential for phage display, plasmid production, and other molecular biology applications.

Unlike 2xYT Agar or 2xYT Top Agar, this broth contains no agar, making it ideal for suspension cultures where cells and phages grow in a well-mixed liquid environment.

Features

Liquid medium – no gelling agents, designed for shaking cultures.
Supports robust growth of E. coli and maintenance of M13 bacteriophage.
Higher nutrient concentration than standard YT broth for improved yield.
Ideal for DNA plasmid preparation, phage production, and protein expression.

Composition (per liter)

Yeast Extract: 10.0 g
Tryptone: 16.0 g
Sodium Chloride (NaCl): 5.0 g
Usage: Dissolve 31.0 g/L in distilled water, autoclave at 121°C for 15 min.

Comparison with 2xYT Agar & 2xYT Top Agar:

Product	Form	Agar Content	Usage
2xYT Broth	Liquid	None	Suspension cultures of E. coli, M13 phage, plasmid production.
2xYT Agar	Solid	15 g/L	Plate cultures for E. coli and M13 phage growth on solid media.
2xYT Top Agar	Semi-solid	7 g/L	Soft agar overlay for phage plaque assays and M13 propagation.

Specifications

Total Product Size: 250 g
Packaging: 1 container (250 g)
Shipping Conditions: Room Temperature (RT)
UNSPSC Code: 41106200 – Microbiological Media

Documents

INFO

COA

SDS

2xYT Broth Medium Powder 250g – Optimized for High-Density E. coli Growth