Intended use

The 1-Step 2X RT-PCR Master Mix-TaqMan probe is used for real-time qualitative and quantitative RT-PCR with TaqMan probe.
The master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers and RNA template.

Characteristics

For the reverse transcription step, this kit uses a highly efficient thermophilic Reverse Transcriptase (US patent pending), which is a thermophilic type A polymerase with optimal temperatures of 60-62°C, and it is heat-inactivated at ≥90°C.
The RTase efficiently synthesizes a complementary DNA strand on RNA template, ≤1 unit per 20 μL of reaction.
The RTase can detect single digit copies of target RNA molecules.
The kit also contains Taq-Probe DNA polymerase for the amplification step, specially engineered for TaqMan probes and generating S-Shaped curves.
The kit is designed for singleplex RT-PCR.
The preferable PCR product size is ≤150bp.

1-Step 2X RT-PCR Master Mix-TaqMan Probe, #Cat. RT-PCR 143