Intended Use

The 1-Step Fast 2X RT-PCR Master Mix is used for real-time qualitative and quantitative RT-PCR amplifications with SYBR Green dye particularly for fast thermocycling.
The master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers and RNA template.

Product Characteristics

The kit is designed for singleplex RT-PCR with SYBR Green dye.
For the reverse transcription step, this kit uses a highly efficient Thermophilic Reverse Transcriptase (US patent pending), which is a thermophilic type A polymerase, with optimal temperatures of 60-62°C.
The RTase is easily heat-inactivated at ≥90°C for 1min.
The RTase efficiently synthesizes a complementary DNA strand on RNA template from a gene-specific primer, ≤1 unit per 20μL of reaction.
The RTase reversely-transcribes single digit copies of target RNA molecules consistently.
The kit also contains Taq-Fast DNA polymerase which extends more than 300 bases with short cycling program.
The concentrations of the primers are variable depending on assay designs and thermocycling protocols (User Manual Table 1).
The preferable PCR product size is ≤150bp.
The kit has three formulations of ROX, Low ROX or High ROX concentrations for your choice.