Intended Use

The 1-Step 2X Fast RT-PCR Master Mix is used for qualitative and quantitative RT-PCR amplifications with SYBR Green dye for fast thermocycling.
The master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers and RNA template.

Kit Characterizations

For the reverse transcription step, this kit uses a highly efficient thermophilic Reverse Transcriptase (US patent pending), which is a thermophilic type A polymerase with optimal temperatures of 60-62°C, and it is heat-inactivated at ≥90°C.
The RTase efficiently synthesizes a complementary DNA strand on RNA template from a gene-specific primer, ≤1 unit per 20μL of reaction.
The RTase can detect single digit copies of target RNA molecules.
The kit also contains Taq-Fast DNA polymerase for the amplification step, which extends more than 300 bases with short cycling program.
The kit is designed for singleplex RT-PCR.
The preferable PCR product size is ≤150bp.

1-Step 2X Fast RT-PCR Master Mix-SYBR Green, #Cat. RT-PCR 147