Description

Zebra Fish Direct PCR Kit uses a unique lysis buffer system to quickly release genomic DNA from zebrafish and other freshwater fish tissues, tail fins or fish eggs samples for PCR reactions, so it is particularly suitable for large-scale genetic testing. The process of releasing genomic DNA from the lysis buffer is completed within 10-30 minutes at 65°C. No other processes such as protein and RNA removal are required, and the released trace DNA can be used as a template for PCR reaction.

2×PCR EasyTM Mix has strong tolerance to PCR reaction inhibitors, and can use the lysate of the sample to be tested as a template for efficient and specific amplification. This reagent contains ForegeneD-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer and stabilizer. Used in conjunction with the lysis buffer can quickly and easily detect samples, and has the characteristics of high sensitivity, strong specificity, and good stability.

D-Taq DNA polymerase is a DNA polymerase specially developed by Foregene for direct PCR reactions. D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems, and the amplification speed can reach 2Kb/min, which is especially suitable for Direct PCR reaction.

Features

No time-consuming and expensive DNA purification is required.
The sample demand is small, as little as 1mg fish caudal fin or 10 fish eggs can be tested.
No special treatments such as grinding and crushing are required, and the operation is simple.
Optimized PCR system makes PCR have higher specificity and stronger tolerance to PCR reaction inhibitors.

Work Flow

Electropherogram

Documents

Zebra Fish Direct PCR Kit Instruction Manual-V1.0-EN

Zebra Fish Direct PCR Kit(65℃ lysis)