Description

This product uses a unique lysis buffer system to quickly release genomic DNA from zebrafish and other freshwater fish tissues, tail fins or fish eggs samples for PCR reactions, so it is particularly suitable for large-scale genetic testing. The process of releasing genomic DNA from the lysis buffer is completed within 10-30 minutes at 65°C. No other processes such as protein and RNA removal are required, and the released trace DNA can be used as a template for PCR reaction.

2×PCR EasyTM Mix has strong tolerance to PCR reaction inhibitors, and can use the lysate of the sample to be tested as a template for efficient and specific amplification. This reagent contains ForegeneD-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer and stabilizer. Used in conjunction with the lysis buffer can quickly and easily detect samples, and has the characteristics of high sensitivity, strong specificity, and good stability.

2×PCR EasyTM Mix (UNG) uses dUTP instead of dTTP on the basis of 2×PCR EasyTM Mix, and adds UNG enzyme (Uracil-N-glycosylase) that can degrade the template containing dUTP at the same time. Before the PCR reaction, the UNG enzyme is used to degrade the PCR product containing uracil. The UNG enzyme will not have any effect on the template that does not contain uracil, thereby ensuring the specificity and accuracy of amplification and preventing the possibility of large-scale genetic testing. Contamination of PCR products occurred.

Taq DNA polymerase is a DNA polymerase specially developed by Foregene for direct PCR reactions. D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems, and the amplification speed can reach 2Kb/min, which is especially suitable for Direct PCR reaction.

Features

No time-consuming and expensive DNA purification is required.
The sample demand is small, as little as 1mg fish caudal fin or 10 fish eggs can be tested.
No special treatments such as grinding and crushing are required, and the operation is simple.
Optimized PCR system makes PCR have higher specificity and stronger tolerance to PCR reaction inhibitors.
Anti-pollution PCR system 2× PCR EasyTM Mix (UNG), which can effectively eliminate the pollution caused by PCR products and ensure the specificity and accuracy of amplification.

Application

Zebra fish and other fresh water fish.

DNA released from sample lysis: only used as a PCR template.

The kit can be used for the following purposes: identification of transgenosis, animal genotyping, etc.

Work Flow

Storage

Part I of this kit should be stored at 2-8℃.

The reagent Buffer FP can be stored for 12 months under dry conditions; it can be stored at 2-8℃ for longer storage.

Reagent Foregene Protease has a unique formula. To ensure its activity and stability, please store it at 4℃ for 12 months.
Reagent 6×DNA Loading Buffer can be stored at 4℃ or -20℃ for a long time.

Part II of this kit should be stored at -20℃ .

vReagent 2×PCR EasyTM Mix(UNG), if is used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days).

Zebra Fish Direct PCR Kit(65℃ lysis)-UNG