Description

YPD Broth is a rich, general-purpose liquid medium designed for the cultivation and propagation of Saccharomyces cerevisiae, Pichia pastoris, and other yeast species. Widely used in molecular cloning, protein expression, and enrichment applications, this medium supports robust yeast growth with a balanced composition: peptic digest of animal tissue supplies essential nitrogenous nutrients, yeast extract contributes both nitrogen and B vitamins, and dextrose serves as a readily metabolizable carbohydrate and energy source. Ideal for high-density yeast cultures in research and industrial workflows.

Specification

Appearance	Light beige, free-flowing powder
pH (5% W/V in water) @25C	6.0-7.0
Solubility (5.0g/100ml Water)	Clear, Yellow to Dark Brown
Usage	Usage: 50.0g/L
Storage	Store at room temperature. Protect from moisture

Components

Ingredients (g/L)
Yeast Extract 10.0g
Peptone 20.0g
D-glucose 20.0g

Application

YPD Broth is recommended for the growth of Saccharomyces cerevisiae and other yeasts for molecular biology purposes. It supports the growth of most heterotrophic microorganisms and, due to its simple yet rich composition, is widely adopted as the basal medium for routine yeast cultivation. Standard methods in yeast genetics specify the use of YPD medium for maintaining and expanding yeast cultures.

Typical Applications

Cultivation and expansion of yeast cells for downstream DNA or protein extraction
Yeast transformation experiments (e.g., LiAc/PEG method)
Fermentation performance testing of yeast strains
Research on fermentation products such as ethanol, aroma compounds, and enzymes
Pre-culture for mutant strain screening and genetic selection

Usage

50 g per liter

Reconstitution

Preparation instructions

Suspend 50 g in 1 L of distilled water.
Autoclave for 15 minutes at 121 °C.

Documents

INFO

SDS

Safety Information

Storage Class

11 - Combustible Solids
Wgk_germany

WGK 3
Flash_point_f

Not applicable
Flash_point_c

Not applicable

Tag： Yeast Cell Culture

YPD BROTH POWDER