Description

YM Medium is a specialized culture medium for Agrobacterium growth, transformation, and gene expression.

It is widely used in Agrobacterium electroporation and Agrobacterium-mediated plant transformation, where it significantly improves transformation efficiency compared with traditional LB broth.

Studies and internal validation show that YM Medium for Agrobacterium can increase electroporation efficiency by up to 40-fold, while effectively reducing bacterial cell aggregation. This results in more uniform cultures, making YM Medium an ideal Agrobacterium transformation medium for plant infection and downstream molecular biology applications.

Specification

Appearance	Light beige, free-flowing powder
Conductivity (1:10) @25C	REPORT
pH (5.6g/ 500ml water)@25C	6.2 ± 0.2
Solubility (5.6g/ 500ml water)	PASS
Supports Growth of Bacteria	PASS
Component (g/L)	Yeast Extract 3.0g Peptone 5.0g D-glucose 10.0g Malt Extract 3.0g
Usage	21.0g/L
Storage	Store at room temperature. Protect from moisture.

Features

Optimized Agrobacterium Culture Medium

Specially formulated as a culture medium for Agrobacterium, supporting robust growth, transformation, and gene expression.
Enhanced Agrobacterium Transformation Efficiency

Significantly improves Agrobacterium electroporation efficiency compared to LB broth, enabling high-yield genetic transformation.
Reduced Cell Aggregation During Culture

Promotes uniform bacterial suspension, which is critical for Agrobacterium-mediated plant transformation and infection studies.
Rich and Balanced Nutrient Composition

Contains yeast extract, peptone, D-glucose, and malt extract to support vigorous and stable Agrobacterium growth.
Easy Preparation and Stable Storage

Supplied as a free-flowing powder; suitable for routine laboratory use and long-term storage at room temperature.

Applications

Cultivation of Agrobacterium species
Agrobacterium-mediated plant transformation
Agrobacterium electroporation and genetic engineering
Plant molecular biology and functional genomics research
Routine microbiological culture for plant infection studies

Storage and Stability

Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.

Documents

COA

MSDS