Description

YM Medium is a specialized culture medium for Agrobacterium growth, transformation, and gene expression.

It is widely used in Agrobacterium electroporation and Agrobacterium-mediated plant transformation, where it significantly improves transformation efficiency compared with traditional LB broth.

Studies and internal validation show that YM Medium for Agrobacterium can increase electroporation efficiency by up to 40-fold, while effectively reducing bacterial cell aggregation. This results in more uniform cultures, making YM Medium an ideal Agrobacterium transformation medium for plant infection and downstream molecular biology applications.

Specification

Appearance	Light beige, free-flowing powder
Conductivity (1:10) @25C	REPORT
pH (5.6g/ 500ml water)@25C	6.2 ± 0.2
Solubility (5.6g/ 500ml water)	PASS
Supports Growth of Bacteria	PASS
Component (g/L)	Yeast Extract 3.0g Peptone 5.0g D-glucose 10.0g Malt Extract 3.0g
Usage	21.0g/L
Storage	Store at room temperature. Protect from moisture.

Features

Optimized Agrobacterium Culture Medium
Specially formulated as a culture medium for Agrobacterium, supporting robust growth, transformation, and gene expression.
Enhanced Agrobacterium Transformation Efficiency
Significantly improves Agrobacterium electroporation efficiency compared to LB broth, enabling high-yield genetic transformation.
Reduced Cell Aggregation During Culture
Promotes uniform bacterial suspension, which is critical for Agrobacterium-mediated plant transformation and infection studies.
Rich and Balanced Nutrient Composition
Contains yeast extract, peptone, D-glucose, and malt extract to support vigorous and stable Agrobacterium growth.
Easy Preparation and Stable Storage
Supplied as a free-flowing powder; suitable for routine laboratory use and long-term storage at room temperature.

Applications

Agrobacterium-mediated plant transformation for T-DNA transfer and stable gene integration studies
Agrobacterium tumefaciens culture and electroporation to improve DNA uptake efficiency in bacterial cells
Plant genetic engineering workflows involving recombinant DNA delivery systems
Plant molecular biology research including gene expression regulation and functional genomics
Plant infection and tissue transformation studies requiring uniform bacterial suspensions
Gene delivery system optimization for improving transformation efficiency and reproducibility
Plant biotechnology applications including floral dip and transient expression systems

Storage and Stability

Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.

Documents

COA MSDS

Frequently Asked Questions

What is YM Medium used for in Agrobacterium research?

YM Medium is used for Agrobacterium culture, electroporation, and Agrobacterium-mediated plant transformation. It provides a nutrient-balanced environment that improves bacterial growth and supports efficient T-DNA transfer in plant genetic engineering workflows.

How does YM Medium improve Agrobacterium transformation efficiency?

YM Medium enhances transformation efficiency by optimizing bacterial physiological state, reducing cell aggregation, and improving DNA uptake during electroporation. This leads to more consistent Agrobacterium-mediated transformation outcomes.

Why is YM Medium better than LB medium for Agrobacterium?

Unlike LB medium, YM Medium is specifically formulated to support Agrobacterium tumefaciens transformation systems. It improves cell uniformity and increases electroporation efficiency, making it more suitable for plant genetic engineering applications.

Can YM Medium be used for electroporation experiments?

Yes. YM Medium is widely used in Agrobacterium electroporation workflows. It supports stable cell preparation and enhances DNA uptake efficiency, improving transformation success rates in molecular biology experiments.

What role does YM Medium play in plant genetic engineering?

YM Medium supports Agrobacterium-mediated gene delivery systems used in plant genetic engineering. It improves T-DNA transfer efficiency, enabling reliable gene expression and functional genomics studies in plant models.

How should YM Medium be prepared for laboratory use?

Dissolve YM Medium at 21 g/L in distilled water, mix thoroughly until fully dissolved, and sterilize according to standard laboratory procedures. Use freshly prepared medium for optimal Agrobacterium growth performance.

For laboratory research or industrial use only.