Description

Fibronectin, also known as FN, is a high-molecular-weight glycoprotein widely found in the extracellular matrix. Its molecular structure contains multiple binding domains that enable selective interactions with various macromolecules such as fibrin, heparin, bacteria, and cell surface receptors. Through these interactions, it participates in numerous physiological processes. Fibronectin plays important roles in mediating cell-matrix and cell-cell adhesion, as well as in regulating cell differentiation, growth, and migration. Furthermore, it is involved in embryonic development, blood coagulation, and wound healing. Additionally, fibronectin plays a critical role in tumor cell metastasis.

Specifications

Product Details
Code	Y13142H
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).Endotoxin≤10 EU/mg by the LAL method.BioactivityMeasured by the ability of the immobilized protein to support the adhesion of NIH3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 4000 ng/mL.AccessionAlternative NamesFN; Cold-insoluble globulin; CIG; FN; Fibronectin 1
Species	Human
Expression System	HEK293
Tag Info	His Tag
Formulation	Lyophilized
Buffer	Lyophilized from a 0.22 μm-filtered solution containing 20 mM Sodium acetate, 5% Mannitol and 0.01% Tween 80, pH2.5.
Size	10μg/50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Hematology

Modulates adhesion of hematopoietic progenitors.

Stem Cell Research

Supports stem cell adhesion, migration, and differentiation on ECM.

Cell Biology

Regulates cell adhesion, migration, and extracellular matrix interactions.

Data Figures

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

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Y13142H Recombinant Human Fibronectin