Description

IL-21 (Interleukin-21) is a pleiotropic cytokine belonging to the common gamma-chain (γc) cytokine family, primarily produced by activated CD4⁺ T cells and natural killer T (NKT) cells. It plays important roles in immune regulation, cell proliferation, differentiation, and functional modulation. In B cell cultures, IL-21 induces plasma cell differentiation and enhances the secretion of IgG and IgM. In T cells, it promotes the proliferation and cytotoxicity of CD8⁺ T cells and NK cells, making it valuable for tumor immunotherapy research, such as in CAR-T cell cultivation. Additionally, IL-21 can suppress regulatory T cell (Treg) function and enhance the anti-tumor activity of effector T cells. In NK cell cultures, it augments killing capacity and IFN-γ secretion, and is widely used in studies related to immune cell therapy.

Specifications

Product Details
Cat. No.	Y02901
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED50 for this effect is ≤ 20 ng/mL.
Accession	Q9HBE4
Alternative Names	Interleukin-3; IL-3; Hematopoietic Growth Factor; Mast Cell Growth Factor; MCGF; Multipotential Colony-Stimulating Factor; P-Cell-Stimulating Factor; IL3
Species	Human
Expression System	CHO
Mol. Weight	15.9 kDa
Expression Region	His25-Ser162
Tag Info	Tag free
Formulation	Lyophilized
Buffer	PBS, 5% Mannitol and 0.01% Tween 80, pH7.4.
Size	10μg/50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at 2°C to 8°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Hematology

Regulates B cell differentiation and antibody production.

Immunology

Enhances T, B, and NK cell effector functions.

Cell Biology

Regulates cell differentiation and effector programs.

Data Figures

Figure 1: Recombinant Human IL-21 (Cat. No. Y02901) SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with Y02901 SDS-PAGE.

Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED50 for this effect is ≤ 20 ng/mL. The bioactivity of recombinant Human IL21 (Cat. No. Y02901) is higher than the other competing product.

Figure 2: Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED50 for this effect is ≤ 20 ng/mL. The bioactivity of recombinant Human IL21 (Cat. No. Y02901) is higher than the other competing product.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

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Y02901 Recombinant Human IL-21