Description

Xylella fastidiosa Probe Realtime PCR Kit is designed for the quantitative detection of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples.

Key Features

One-tube qPCR format minimizes carry-over contamination risk

High sensitivity: analytical LOD as low as 100 copies/reaction
Excellent specificity: no cross-reactivity with DNA from other pathogens
Includes amplification positive control for result verification
Instrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc.
Suitable for both qualitative and quantitative applications
For quantitative use: the linear range covers a 100,000-fold concentration difference.
Sufficient for 50 reactions (20 μL each)
For Research Use Only (RUO)

Kit Components

Component Name	Product Number.	Size	Vial	Lid Color
2×Probe qPCR MasterMix	981201	0.5 mL	0.5 mL Vial	Natural
Template Dilution Buffer	180701	1 mL	1.5 mL Vial	Green
Primer-Probe Powder	yp15-5215	50 T	0.5 mL Brown Vial	Brown
Amplification Positive Control (1E7 copies/μL)	pc5215	50 μL	0.5 mL Vial	Yellow
Ultrapure water	210806	1 mL	1.5 mL Vial	Blue
Manual	sc15-5215	1 Set	NA	NA

Application Areas

Crop Health Monitoring & Disease Management: High-sensitivity screening of grapevine, citrus and ornamental plants for early Xylella fastidiosa detection to support precision agriculture and minimize yield losses.
Phytosanitary Certification & Biosecurity Compliance: Robust one-tube qPCR testing in nurseries and quarantine facilities for pathogen-free plant material, ensuring compliance with international phytosanitary regulations.
Plant Pathology Research & Resistance Breeding: Quantitative analysis of bacterial load in host tissues for R&D—accelerating varietal resistance trials, epidemiological studies and vector transmission research.

Storage & Shipping

Store at - 20 °C
The product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.

Documents

Xylella fastidiosa Probe Realtime PCR Kit User Manual

MSDS