{"product_id":"xr-phenol-red-free-matrigel","title":"XR Phenol Red-Free Matrigel","description":"\u003c!--\nSEO TITLE:\nLow Growth Factor Phenol Red-Free Matrigel | XRMat-0-10\n\nMETA DESCRIPTION:\nReduce color interference and growth factor background in organoid and 3D cell culture with a phenol red-free basement membrane matrix supplied at 8–12 mg\/mL.\n\nRECOMMENDED URL:\n\/products\/low-growth-factor-phenol-red-free-matrigel\n\nPRIMARY KEYWORDS:\nLow Growth Factor Matrigel\nPhenol Red-Free Matrigel\nGrowth Factor Reduced Basement Membrane Matrix\nPhenol Red-Free Basement Membrane Matrix\nBasement Membrane Matrix\nOrganoid Culture Matrix\n\nSECONDARY KEYWORDS:\n3D Cell Culture Matrix\nBasement Membrane Extract\nExtracellular Matrix Hydrogel\nECM Matrix for Organoid Culture\nLaminin-Rich Matrix\nOrganoid Extracellular Matrix\nPhenol Red-Free ECM Matrix\nReduced Growth Factor ECM\nBasement Membrane Hydrogel\nCell Culture Matrix\n--\u003e\n\u003cstyle\u003e\n  \/* 现代专业风全局样式 *\/\n  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}\n\u003c\/style\u003e\n\u003cdiv class=\"pdp-container\"\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eColor interference, undefined growth factor activity, and inconsistent gel formation can complicate organoid and three-dimensional cell culture workflows. These variables may make it more difficult to distinguish experimental responses from signals introduced by the extracellular matrix itself, particularly in fluorescence-based assays and studies that require controlled growth factor supplementation.\u003c\/p\u003e\n\u003cp\u003eXR Low Growth Factor Phenol Red-Free Matrigel, XRMat-0-10, is a natural basement membrane matrix extracted from mouse tumor tissue rich in extracellular matrix proteins. The formulation provides a laminin-rich three-dimensional matrix containing collagen IV, entactin and heparan sulfate proteoglycans, creating a basement membrane-like environment for cell embedding, matrix coating and organoid culture research.\u003c\/p\u003e\n\u003cp\u003eThis phenol red-free basement membrane matrix is formulated to reduce visible color interference while limiting background signals associated with endogenous growth factors. The product is supplied in high-glucose, phenol red-free DMEM and has a protein concentration of 8–12 mg\/mL. At 4°C, the thawed matrix remains liquid for handling and dispensing; after warming, it forms a stable extracellular matrix hydrogel, with gelation occurring more rapidly at 37°C.\u003c\/p\u003e\n\u003cp\u003eXRMat-0-10 is intended for research workflows requiring a low growth factor Matrigel-type matrix, including organoid culture, 3D cell culture, fluorescence-compatible culture systems and studies in which researchers need greater control over externally added growth factors.\u003c\/p\u003e\n\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cdiv class=\"pdp-table-responsive\"\u003e\n\u003ctable class=\"pdp-table\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eXR Low Growth Factor Phenol Red-Free Matrigel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eXRMat-0-10\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eProduct Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eLow growth factor phenol red-free basement membrane matrix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix Source\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMouse tumor-derived basement membrane extract\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eProtein Concentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e8–12 mg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eAppearance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eTranslucent, pale yellow\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePhysical State\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eLiquid after thawing at 4°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDiluent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHigh-glucose, phenol red-free DMEM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eAntibiotic\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e50 μg\/mL gentamicin in standard, non-custom formulations\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eEndotoxin\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e≤10 EU\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGelation Time\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eApproximately 5–30 minutes at room temperature; gelation is faster at 37°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e≤−20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eShipping Condition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFrozen, ≤−20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eShelf Life\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGrade\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFor research use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch2\u003eMatrix Composition\u003c\/h2\u003e\n\u003cp\u003eXRMat-0-10 is a naturally derived basement membrane extract containing extracellular matrix proteins and associated biological components. Its principal structural components include:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eLaminin, a major basement membrane glycoprotein involved in cell adhesion and matrix organization\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eCollagen IV, a structural collagen that contributes to the basement membrane network\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eEntactin, also known as nidogen, which connects laminin and collagen IV networks\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eHeparan sulfate proteoglycans, which contribute to extracellular matrix structure and molecular interactions\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eAs a natural tumor-derived extracellular matrix, the product may also contain residual endogenous factors, including IGF-1, TGF-β, VEGF, EGF and bFGF. The low growth factor formulation is designed to reduce, rather than completely eliminate, growth factor-associated background activity.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eLow growth factor basement membrane matrix for experiments requiring reduced endogenous signaling background\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003ePhenol red-free formulation helps minimize visible color and fluorescence interference\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eContains key basement membrane proteins, including laminin, collagen IV, entactin and heparan sulfate proteoglycans\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eProtein concentration controlled within an 8–12 mg\/mL range\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eRemains liquid when properly thawed and handled at 4°C\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eTemperature-responsive gelation supports convenient cold handling and rapid matrix formation after warming\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSuitable for organoid embedding and three-dimensional extracellular matrix culture workflows\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSupplied in high-glucose, phenol red-free DMEM\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eEach batch is evaluated in an organoid culture test\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eTested for mycoplasma, bacterial and fungal contamination\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eBroad PCR-based pathogen testing includes LDEV\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eQuality Control\u003c\/h2\u003e\n\u003cdiv class=\"pdp-table-responsive\"\u003e\n\u003ctable class=\"pdp-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eQuality Attribute\u003c\/th\u003e\n\u003cth\u003eTest or Acceptance Criterion\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eMycoplasma\u003c\/td\u003e\n\u003ctd\u003eNot detected\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBacterial Contamination\u003c\/td\u003e\n\u003ctd\u003eNot detected\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFungal Contamination\u003c\/td\u003e\n\u003ctd\u003eNot detected\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEndotoxin\u003c\/td\u003e\n\u003ctd\u003eTested using a gel-clot limit test; product specification ≤10 EU\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProtein Concentration\u003c\/td\u003e\n\u003ctd\u003eMeasured by BCA assay\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePathogen Screening\u003c\/td\u003e\n\u003ctd\u003eBroad PCR-based testing for multiple pathogens, including LDEV\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFunctional Evaluation\u003c\/td\u003e\n\u003ctd\u003eEach batch is evaluated using an organoid culture test\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch2\u003eGelation and Stability\u003c\/h2\u003e\n\u003cp\u003eXRMat-0-10 is temperature-sensitive. The thawed material remains liquid at 4°C and begins to form a basement membrane hydrogel as the temperature increases. At room temperature, gelation generally occurs within approximately 5–30 minutes, while incubation at 37°C accelerates gel formation.\u003c\/p\u003e\n\u003ch3\u003eEvaluated Dilution Condition\u003c\/h3\u003e\n\u003cp\u003eWhen the matrix was diluted with culture medium at a 1:2 ratio and incubated at 37°C for 30 minutes, it formed a gel. After culture medium was added, the matrix retained its gel structure for five days under the evaluated 37°C condition.\u003c\/p\u003e\n\u003ch3\u003eEvaluated 70% Matrix Condition\u003c\/h3\u003e\n\u003cp\u003eWhen diluted to a final matrix content of 70%, dispensed at 50 μL in a cell culture plate and incubated at 37°C for 30 minutes, the product formed a stable gel. After culture medium was added, the gel structure was maintained for up to 15 days under the evaluated 37°C culture condition.\u003c\/p\u003e\n\u003cdiv class=\"pdp-note\"\u003e\n\u003cp\u003e\u003cstrong\u003eImportant:\u003c\/strong\u003e The evaluated dilution and gel stability conditions are reference data rather than a universal protocol. Optimal matrix concentration, volume and gelation time should be determined for each cell type, culture vessel and experimental workflow.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eXR Low Growth Factor Phenol Red-Free Matrigel is designed for research applications that require a laminin-rich extracellular matrix while minimizing phenol red-related color interference and reducing the contribution of endogenous growth factors. It can be incorporated into matrix coating, cell embedding and three-dimensional culture workflows according to laboratory-specific protocols.\u003c\/p\u003e\n\u003ch3\u003eOrganoid Culture and Expansion\u003c\/h3\u003e\n\u003cp\u003eThis organoid culture matrix provides a three-dimensional basement membrane-like environment for embedding organoids and supporting spatial tissue organization. Each production batch is evaluated in an organoid culture test, making XRMat-0-10 suitable for laboratories developing or maintaining organoid workflows.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eHuman or animal organoid culture research\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eOrganoid establishment and three-dimensional expansion\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eEmbedding organoids within a basement membrane hydrogel\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eLong-term organoid culture workflows requiring a stable matrix structure\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eOrganoid morphology and imaging studies\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003e3D Cell Culture\u003c\/h3\u003e\n\u003cp\u003eThe matrix can be used as a three-dimensional cell culture scaffold where cells require extracellular matrix proteins such as laminin and collagen IV. Its temperature-dependent gelation allows the product to be handled as a cold liquid and converted into a semi-solid matrix after incubation.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eThree-dimensional cell embedding\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eBasement membrane matrix coating\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eCell–matrix interaction studies\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eExtracellular matrix-dependent cell morphology research\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eCulture models requiring a laminin-rich ECM matrix\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eGrowth Factor-Controlled Research\u003c\/h3\u003e\n\u003cp\u003eEndogenous growth factors present in natural basement membrane extracts may complicate experiments that evaluate defined signaling conditions. The reduced growth factor formulation helps researchers limit this background contribution when studying the effects of separately added growth factors, inhibitors or other experimental treatments.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eStudies involving controlled addition of exogenous growth factors\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eCell signaling research requiring reduced matrix-derived background\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eComparative culture studies using defined treatment conditions\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eExperimental workflows sensitive to endogenous matrix-associated factors\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eFluorescence-Compatible Culture Workflows\u003c\/h3\u003e\n\u003cp\u003ePhenol red may contribute visible color and background interference in some imaging and optical detection workflows. The phenol red-free formulation is appropriate for laboratories seeking a basement membrane matrix with reduced color interference.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eFluorescence microscopy of matrix-embedded cells or organoids\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eLive-cell imaging research\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eConfocal imaging of three-dimensional cultures\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eImage-based analysis where phenol red is undesirable\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eCancer and Tumor Model Research\u003c\/h3\u003e\n\u003cp\u003eXRMat-0-10 may be used as an extracellular matrix scaffold in research models involving tumor-derived cells or organoids when a low growth factor, phenol red-free matrix is preferred. Researchers should optimize matrix concentration and culture conditions for the specific model.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eTumor organoid culture research\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThree-dimensional cancer cell culture\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003ePatient-derived organoid research workflows\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eDrug-response studies using matrix-embedded organoids\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eRecommended Handling\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eStore the unopened product at or below −20°C.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThaw the matrix under refrigerated conditions at approximately 4°C.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eKeep the product, pipette tips, tubes and culture vessels cold during preparation to reduce premature gelation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eMix gently while cold. Avoid vigorous agitation that may introduce bubbles.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eDispense the required volume while the matrix remains liquid.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eIncubate at the temperature and duration established for the specific culture protocol.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eBecause the matrix is biologically derived, optimize concentration and handling conditions for each cell line or organoid model.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv class=\"pdp-note\"\u003e\n\u003cp\u003e\u003cstrong\u003eFormulation note:\u003c\/strong\u003e Standard non-custom products contain 50 μg\/mL gentamicin. Researchers performing antibiotic-free experiments should consider this formulation detail during experimental planning.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch2\u003eWhy Choose a Low Growth Factor Phenol Red-Free Matrix?\u003c\/h2\u003e\n\u003cp\u003eA standard basement membrane extract can provide a supportive extracellular environment, but its native growth factors and color components may introduce variables into sensitive experiments. These effects are particularly relevant when investigators are adding defined signaling molecules, quantifying fluorescence or comparing treatment-dependent responses.\u003c\/p\u003e\n\u003cp\u003eXRMat-0-10 combines a low growth factor formulation with a phenol red-free diluent to address these practical laboratory concerns. The matrix retains the key structural proteins expected from a natural basement membrane extract while providing a more suitable format for growth factor-sensitive studies, organoid imaging and three-dimensional cell culture workflows.\u003c\/p\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is XR Low Growth Factor Phenol Red-Free Matrigel used for?\u003c\/summary\u003e\n\u003cp\u003eXRMat-0-10 is a natural basement membrane matrix used in research workflows such as organoid culture, three-dimensional cell culture, extracellular matrix coating, cell embedding and fluorescence-based imaging studies.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the difference between low growth factor Matrigel and a standard basement membrane matrix?\u003c\/summary\u003e\n\u003cp\u003eA low growth factor matrix is formulated to reduce the amount of endogenous growth factors associated with natural basement membrane extracts. This can help reduce matrix-derived background when researchers are studying defined growth factor conditions or signaling responses. It does not mean that all endogenous growth factors are completely absent.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhy is a phenol red-free basement membrane matrix useful?\u003c\/summary\u003e\n\u003cp\u003eA phenol red-free basement membrane matrix helps minimize visible color and potential optical background from phenol red. This may be beneficial for fluorescence microscopy, confocal imaging, live-cell imaging and other image-based assays involving cells or organoids embedded in an extracellular matrix hydrogel.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eIs XRMat-0-10 suitable for organoid culture?\u003c\/summary\u003e\n\u003cp\u003eYes. XRMat-0-10 is suitable for organoid culture research, and each batch is evaluated using an organoid culture test. The optimal matrix concentration, droplet volume and culture protocol should be established for the specific organoid type.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat extracellular matrix proteins are present in XRMat-0-10?\u003c\/summary\u003e\n\u003cp\u003eThe principal components include laminin, collagen IV, entactin or nidogen, and heparan sulfate proteoglycans. These proteins contribute to the basement membrane-like structure of the matrix.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eDoes the product contain any growth factors?\u003c\/summary\u003e\n\u003cp\u003eThe product is a low growth factor formulation rather than a completely growth factor-free matrix. As a naturally derived basement membrane extract, it may contain residual factors such as IGF-1, TGF-β, VEGF, EGF and bFGF.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the protein concentration of XRMat-0-10?\u003c\/summary\u003e\n\u003cp\u003eThe specified protein concentration range is 8–12 mg\/mL, measured using the BCA method.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow quickly does the basement membrane matrix gel?\u003c\/summary\u003e\n\u003cp\u003eThe matrix generally gels within approximately 5–30 minutes at room temperature. Gelation occurs more rapidly at 37°C. Actual gelation time may vary with matrix concentration, dispensed volume, vessel temperature and handling conditions.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow should phenol red-free Matrigel be thawed?\u003c\/summary\u003e\n\u003cp\u003eThe product should be thawed under refrigerated conditions at approximately 4°C. It should be kept cold during preparation because warming can initiate gelation.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan XRMat-0-10 be diluted with culture medium?\u003c\/summary\u003e\n\u003cp\u003eYes. The product documentation includes evaluated diluted conditions, including a 1:2 matrix-to-medium condition and a 70% matrix condition. However, dilution can alter gel strength and stability, so the appropriate concentration should be optimized for each application.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow stable is the gel after polymerization?\u003c\/summary\u003e\n\u003cp\u003eUnder an evaluated 1:2 dilution condition, the gel maintained its structure for five days at 37°C after medium addition. Under an evaluated 70% matrix condition, the gel maintained its structure for up to 15 days. These results are reference data and may vary with the culture system.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eDoes XRMat-0-10 contain antibiotics?\u003c\/summary\u003e\n\u003cp\u003eStandard non-custom formulations contain 50 μg\/mL gentamicin. This should be considered when planning antibiotic-free or antimicrobial-sensitive experiments.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat contamination and pathogen testing is performed?\u003c\/summary\u003e\n\u003cp\u003eThe product is tested for mycoplasma, bacterial and fungal contamination. Broad PCR-based pathogen testing is also performed and includes LDEV screening.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the endotoxin specification?\u003c\/summary\u003e\n\u003cp\u003eThe endotoxin specification is ≤10 EU\/mL.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow should XRMat-0-10 be stored?\u003c\/summary\u003e\n\u003cp\u003eThe product should be stored frozen at or below −20°C. The stated shelf life is 24 months when stored under the recommended conditions.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eIs this product intended for clinical or diagnostic use?\u003c\/summary\u003e\n\u003cp\u003eNo. XRMat-0-10 is intended for research use only and is not intended for diagnostic, therapeutic or clinical applications.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cp\u003e\u003cem\u003eFor Research Use Only. Not for use in diagnostic procedures.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003cscript type=\"application\/ld+json\"\u003e\n{\n  \"@context\": \"https:\/\/schema.org\",\n  \"@type\": \"Product\",\n  \"name\": \"XR Low Growth Factor Phenol Red-Free Matrigel\",\n  \"sku\": \"XRMat-0-10\",\n  \"mpn\": \"XRMat-0-10\",\n  \"description\": \"A low growth factor phenol red-free basement membrane matrix for organoid culture, three-dimensional cell culture, extracellular matrix coating and fluorescence-compatible research workflows. Supplied at a protein concentration of 8–12 mg\/mL in high-glucose phenol red-free DMEM.\",\n  \"category\": \"Basement Membrane Matrix\",\n  \"material\": \"Mouse tumor-derived basement membrane extract\",\n  \"additionalProperty\": [\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Size\",\n      \"value\": \"10 mL\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Protein Concentration\",\n      \"value\": \"8–12 mg\/mL\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Endotoxin\",\n      \"value\": \"≤10 EU\/mL\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Diluent\",\n      \"value\": \"High-glucose phenol red-free DMEM\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Gelation Time\",\n      \"value\": \"Approximately 5–30 minutes at room temperature; faster at 37°C\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Storage\",\n      \"value\": \"≤−20°C\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Shelf Life\",\n      \"value\": \"24 months\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Research Grade\",\n      \"value\": \"For research use only\"\n    }\n  ]\n}\n\u003c\/script\u003e \u003cscript type=\"application\/ld+json\"\u003e\n{\n  \"@context\": \"https:\/\/schema.org\",\n  \"@type\": \"FAQPage\",\n  \"mainEntity\": [\n    {\n      \"@type\": \"Question\",\n      \"name\": \"What is XR Low Growth Factor Phenol Red-Free Matrigel used for?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"XRMat-0-10 is a natural basement membrane matrix used in research workflows such as organoid culture, three-dimensional cell culture, extracellular matrix coating, cell embedding and fluorescence-based imaging studies.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"What is the difference between low growth factor Matrigel and a standard basement membrane matrix?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"A low growth factor matrix is formulated to reduce the amount of endogenous growth factors associated with natural basement membrane extracts. This can help reduce matrix-derived background when researchers are studying defined growth factor conditions or signaling responses.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"Why is a phenol red-free basement membrane matrix useful?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"A phenol red-free basement membrane matrix helps minimize visible color and potential optical background from phenol red. This may benefit fluorescence microscopy, confocal imaging, live-cell imaging and other image-based assays.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"Is XRMat-0-10 suitable for organoid culture?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"Yes. XRMat-0-10 is suitable for organoid culture research, and each batch is evaluated using an organoid culture test. Optimal matrix concentration and culture conditions should be established for each organoid type.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"What extracellular matrix proteins are present in XRMat-0-10?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"The principal components include laminin, collagen IV, entactin or nidogen, and heparan sulfate proteoglycans.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"Does the product contain any growth factors?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"The product is a low growth factor formulation rather than a completely growth factor-free matrix. It may contain residual factors such as IGF-1, TGF-beta, VEGF, EGF and bFGF.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"What is the protein concentration of XRMat-0-10?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"The specified protein concentration range is 8–12 mg\/mL, measured using the BCA method.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"How quickly does the basement membrane matrix gel?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"The matrix generally gels within approximately 5–30 minutes at room temperature. Gelation occurs more rapidly at 37°C.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"How should phenol red-free Matrigel be thawed?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"The product should be thawed under refrigerated conditions at approximately 4°C and kept cold during preparation to reduce premature gelation.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"Does XRMat-0-10 contain antibiotics?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"Standard non-custom formulations contain 50 micrograms per milliliter gentamicin.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"What contamination and pathogen testing is performed?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"The product is tested for mycoplasma, bacterial and fungal contamination. Broad PCR-based pathogen testing is also performed and includes LDEV screening.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"How should XRMat-0-10 be stored?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"The product should be stored frozen at or below -20°C. The stated shelf life is 24 months under the recommended storage conditions.\"\n      }\n    }\n  ]\n}\n\u003c\/script\u003e\u003c\/p\u003e","brand":"XRbio","offers":[{"title":"10mL","offer_id":52798321983669,"sku":"XRMat-0-10","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/2_6fbe2a09-67d2-45f7-8abb-3a02c6873d87.jpg?v=1784009657","url":"https:\/\/biofargo.com\/products\/xr-phenol-red-free-matrigel","provider":"Biofargo","version":"1.0","type":"link"}