Description

Establishing organoid cultures from primary tissue requires sufficient tissue dissociation without excessive digestion. Incomplete digestion may leave large tissue fragments that are difficult to process, while overly aggressive treatment can reduce cell viability, damage important cell populations, and compromise the ability of isolated cells or cell clusters to form organoids.

XR Organoid Tissue Digestion Working Solution is a ready-to-use enzymatic tissue dissociation reagent developed for organoid sample preparation. Its optimized formulation supports rapid and gentle tissue digestion while helping minimize cell damage caused by overdigestion.

The solution is designed to preserve the viability and functional characteristics of key tissue-derived cell populations during organoid tissue dissociation. Following digestion, the recovered cells or cell clusters can be combined with an appropriate organoid culture medium and extracellular matrix for subsequent organoid establishment.

The workflow has been evaluated with lung, liver, kidney, gastric and tumor tissues. Its low-endotoxin formulation is suitable for organoid research workflows in which unnecessary interference with cellular signaling or transcriptomic analysis should be minimized. The digestion procedure is also designed to help preserve cell-surface antigens and functional proteins for downstream flow cytometry and immunofluorescence analysis.

Specifications

Product Name	XR Organoid Tissue Digestion Working Solution
Product Type	Ready-to-use organoid tissue digestion solution
Format	Working solution
Pack Size	100 mL
Endotoxin	≤0.1 EU/mL
Filtration	<0.2 μm
Validated Tissue Types	Lung, liver, kidney, gastric and tumor tissues
Bacteria Detection	Not detected
Fungi Detection	Not detected
Storage	15–25°C
Shelf Life	12 months
Grade	For research use only

Features

Ready-to-use organoid tissue digestion working solution requiring no additional preparation before use
Optimized for rapid and gentle enzymatic tissue dissociation
Helps reduce cell damage associated with excessive tissue digestion
Supports recovery of viable cells and cell clusters for organoid establishment
Evaluated with lung, liver, kidney, gastric and tumor tissue samples
Low-endotoxin formulation with an endotoxin specification of ≤0.1 EU/mL
Designed to preserve cell-surface antigens and functional proteins during tissue processing
Compatible with downstream flow cytometry and immunofluorescence workflows
Suitable for organoid sample preparation and tissue-derived cell recovery

Recommended Workflow

The following procedure provides a general starting workflow for organoid tissue dissociation. Digestion time should be adjusted according to tissue source, tissue size and the degree of dissociation required for the intended culture workflow.

Use surgical scissors to cut the tissue into small tissue fragments.
Transfer the tissue fragments to a 15 mL centrifuge tube.
Add 10 mL of XR Organoid Tissue Digestion Working Solution.
Incubate at 37°C for 15–45 minutes, depending on tissue source and fragment size.
Gently pipette or agitate the sample every 10 minutes during digestion.
Add an appropriate digestion stop solution when the required dissociation level has been reached.
Pass the tissue suspension through a 100 μm cell strainer.
Centrifuge the filtrate and collect the cell pellet.
Assess the amount of red blood cells in the pellet and perform red blood cell lysis when required.

Optimization note: The stated 15–45 minute digestion range is a reference starting point rather than a universal protocol. Tissue density, fragment size and sample origin can affect digestion efficiency. Monitor the sample during incubation and stop digestion when the desired cell or cell-cluster size has been obtained.

Performance Data

Rapid Tissue Dissociation and Cell-Cluster Recovery

In the documented evaluation, minced tissue samples were incubated with the organoid tissue digestion solution at 37°C. A digestion period of approximately 25–35 minutes was sufficient to complete tissue dissociation under the tested conditions.

The resulting cells and cell clusters retained strong viability and could be combined with an appropriate organoid culture medium and matrix to support subsequent formation of tissue-specific organoids.

Liver lung kidney and gastric tissue digestion results using organoid tissue dissociation solution

Representative tissue digestion results for liver, lung, kidney and gastric samples under the tested conditions.

Applications

XR Organoid Tissue Digestion Working Solution is intended for laboratory workflows requiring gentle tissue dissociation before organoid culture or downstream cellular analysis. It can be used as the tissue-processing step between specimen preparation and organoid establishment.

Organoid Establishment from Primary Tissue

The solution can be used to digest minced primary tissue into viable cells or cell clusters before embedding in an appropriate extracellular matrix. This application supports organoid establishment workflows in which controlled tissue dissociation is needed to obtain suitable starting material without unnecessary overdigestion.

Lung Organoid Tissue Dissociation

Suitable for processing lung tissue before lung organoid culture. The gentle digestion workflow helps release tissue-derived cells and cell clusters for subsequent culture in a compatible lung organoid medium and matrix system.

Liver Organoid Tissue Dissociation

Can be used for enzymatic dissociation of liver tissue during liver organoid sample preparation. Digestion time should be optimized according to tissue density, fragment size and the required degree of dissociation.

Kidney Organoid Tissue Dissociation

Applicable to kidney tissue digestion before renal organoid establishment. The recovered cells or cell clusters can be collected by filtration and centrifugation before transfer into the selected kidney organoid culture workflow.

Gastric Organoid Tissue Dissociation

Supports gastric tissue processing for stomach or gastric organoid research. The working solution provides a practical digestion step for preparing tissue-derived material prior to matrix embedding and organoid culture.

Tumor Organoid Sample Preparation

The solution has also been evaluated with tumor tissue and can be used during tumor organoid tissue dissociation. Because tumor specimens can vary substantially in fibrosis, necrosis and tissue density, digestion progress should be monitored and adjusted for each sample.

Cell and Cell-Cluster Preparation

The digestion and filtration workflow can be used to prepare cells or small cell clusters from tissue samples. The required dissociation level should be selected according to the downstream application rather than assuming that every workflow requires a completely single-cell suspension.

Flow Cytometry Sample Preparation

The formulation is designed to help preserve cell-surface antigens and is compatible with downstream flow cytometry workflows. Marker preservation and staining performance should still be confirmed for the specific tissue type, antibody panel and incubation conditions used in each experiment.

Immunofluorescence Sample Preparation

Recovered cells or cell clusters may be used in downstream immunofluorescence analysis. The gentle tissue dissociation approach is intended to reduce unnecessary damage to functional proteins during sample processing.

Transcriptomic Research Workflows

The low-endotoxin formulation is beneficial for research workflows in which endotoxin-associated changes in cellular signaling or gene expression are a concern. Sample handling, digestion duration and downstream processing should be standardized when comparing transcriptomic datasets.

Why Choose XR Organoid Tissue Digestion Solution?

Organoid establishment depends not only on the culture medium and matrix but also on the quality of the starting tissue preparation. A digestion step that is too weak may produce large residual fragments, while prolonged or aggressive treatment may reduce cell recovery and compromise organoid-forming capacity.

XR Organoid Tissue Digestion Working Solution provides a ready-to-use workflow for researchers who need controlled enzymatic tissue dissociation across several organoid tissue models. Its combination of gentle digestion, low endotoxin, room-temperature storage and compatibility with downstream cellular analysis makes it suitable for routine organoid sample preparation.

FAQ

What is XR Organoid Tissue Digestion Working Solution used for?

It is used to enzymatically dissociate minced tissue into viable cells or cell clusters before organoid establishment, matrix embedding or downstream cellular analysis.

Is this product an organoid dissociation reagent?

Yes. It is a ready-to-use organoid tissue dissociation reagent developed for processing primary tissue before organoid culture. It is intended for tissue digestion rather than routine detachment of standard two-dimensional cell cultures.

Which tissue types have been evaluated with this digestion solution?

The product documentation identifies lung, liver, kidney, gastric and tumor tissues as evaluated tissue types.

How much organoid tissue digestion solution should be used?

The documented starting procedure uses 10 mL of digestion solution for minced tissue placed in a 15 mL centrifuge tube. The required volume may need to be adjusted according to tissue amount and vessel size.

How long should tissue be digested?

The recommended starting range is 15–45 minutes at 37°C. A period of approximately 25–35 minutes completed digestion under the documented test conditions, but the optimal time depends on tissue source, fragment size and tissue density.

Should the sample be mixed during tissue digestion?

Yes. The documented procedure recommends gentle pipetting or agitation every 10 minutes during incubation to promote uniform tissue dissociation.

How is the digestion reaction stopped?

After the required degree of tissue dissociation has been reached, add an appropriate digestion stop solution. The product documentation does not specify the composition of the stop solution, so laboratories should use a compatible stopping method for their validated workflow.

Does the workflow produce a single-cell suspension?

The product is designed to generate viable cells and cell clusters from tissue. The final degree of dissociation depends on tissue type, incubation time and mechanical handling. Researchers requiring a predominantly single-cell suspension should optimize and verify the procedure for their specific sample.

Can this reagent be used for tumor organoid tissue dissociation?

Yes. Tumor tissue is included among the evaluated tissue types. Because tumor samples vary in density and composition, digestion time should be monitored and optimized for each specimen.

Is the digestion solution compatible with flow cytometry?

The formulation is designed to preserve cell-surface antigens and is compatible with flow cytometry sample preparation. Researchers should confirm recovery of the specific markers used in their antibody panel.

Can digested samples be used for immunofluorescence?

Yes. The product is designed to help preserve functional proteins and can be incorporated into sample-preparation workflows for downstream immunofluorescence analysis.

What is the endotoxin level of the product?

The specified endotoxin level is ≤0.1 EU/mL. The low-endotoxin formulation helps reduce an avoidable source of interference in cell-signaling and transcriptomic research workflows.

How should XR Organoid Tissue Digestion Working Solution be stored?

Store the product at 15–25°C. The documented shelf life is 12 months when stored under the recommended conditions.

Is the product supplied as a concentrate?

No. It is supplied as a working solution and is designed to be used directly in the tissue digestion workflow.

What should be done after tissue digestion?

Stop the digestion, pass the tissue suspension through a 100 μm cell strainer, centrifuge to collect the cell pellet and assess whether red blood cell lysis is required. The recovered material can then be transferred into an appropriate organoid culture workflow.

For Research Use Only. Not intended for diagnostic, therapeutic or clinical use.