{"product_id":"xr-msc-max-kit-human-msc-serum-free-medium-kit","title":"XR MSC Max Kit – Human MSC Serum-Free Medium Kit","description":"\u003c!--\nSEO TITLE:\nHuman MSC Serum-Free Culture Kit | XR MSC Max\n\nMETA DESCRIPTION:\nImprove inconsistent human MSC expansion with a serum-free, animal component-free culture kit designed for long-term growth and multipotency maintenance.\n\nRECOMMENDED URL:\n\/products\/human-msc-serum-free-culture-kit\n\nPRIMARY KEYWORDS:\nHuman MSC Serum-Free Culture Kit\nHuman MSC Serum-Free Medium\nMSC Expansion Medium\nMesenchymal Stem Cell Growth Medium\nHuman Mesenchymal Stem Cell Medium\nSerum-Free MSC Medium\n\nSECONDARY KEYWORDS:\nHuman MSC Culture Medium\nMSC Culture Kit\nHuman MSC Expansion Kit\nMesenchymal Stem Cell Expansion Medium\nAnimal Component-Free MSC Medium\nXeno-Free MSC Culture Medium\nLong-Term MSC Culture\nUmbilical Cord MSC Culture\nBone Marrow MSC Culture\nAdipose-Derived MSC Culture\nMSC Proliferation Medium\nMSC Multipotency\nMSC Three-Lineage Differentiation\nHuman Mesenchymal Stromal Cell Culture\n--\u003e\n\u003cstyle\u003e\n  \/* 现代专业风全局样式 *\/\n  .pdp-container {\n    font-family: -apple-system, BlinkMacSystemFont, \"Segoe UI\", Roboto, Helvetica, Arial, sans-serif;\n    color: #333333;\n    line-height: 1.6;\n    max-width: 1200px;\n    margin: 0 auto;\n    padding: 15px;\n  }\n  \n  \/* 标题样式升级 *\/\n  .pdp-container h2 {\n    font-size: 24px;\n    color: #0B3C5D;\n    border-bottom: 2px solid #328CC1;\n    padding-bottom: 8px;\n    margin-top: 40px;\n    margin-bottom: 20px;\n    font-weight: 600;\n  }\n  \n  .pdp-container h3 {\n    font-size: 18px;\n    color: #1D2731;\n    margin-top: 30px;\n    margin-bottom: 15px;\n    font-weight: 600;\n  }\n  \n  \/* 段落与列表 *\/\n  .pdp-container p {\n    margin-bottom: 16px;\n    font-size: 15px;\n    color: #4A4A4A;\n    text-align: justify;\n  }\n  \n  .pdp-container ul {\n    padding-left: 20px;\n    margin-bottom: 24px;\n  }\n  \n  .pdp-container li {\n    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}\n\u003c\/style\u003e\n\u003cdiv class=\"pdp-container\"\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eMaintaining reliable human mesenchymal stem cell expansion can be difficult when serum- or platelet lysate-based culture systems introduce lot-to-lot variability, inconsistent proliferation, or changes in cell characteristics during serial passage. Laboratories working with umbilical cord, bone marrow, or adipose-derived MSCs need a more standardized culture environment that supports cell yield without compromising multipotency.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eXR MSC Max Kit\u003c\/strong\u003e is a serum-free human mesenchymal stem cell culture kit designed for the isolation and continuous expansion of human MSCs. The kit combines a dedicated basal medium with an optimized supplement to provide a complete \u003cstrong\u003ehuman MSC serum-free medium\u003c\/strong\u003e for routine propagation and long-term culture.\u003c\/p\u003e\n\u003cp\u003eThe animal component-free formulation provides an alternative to conventional αMEM plus human platelet lysate culture workflows. Under the evaluated conditions, the medium supported repeated 72-hour passages, sustained expansion through serial culture, maintenance of representative MSC phenotypes, and adipogenic, osteogenic, and chondrogenic differentiation potential.\u003c\/p\u003e\n\u003cp\u003eXR MSC Max Kit is suitable for laboratories seeking a consistent \u003cstrong\u003emesenchymal stem cell growth medium\u003c\/strong\u003e for research involving human umbilical cord MSCs, bone marrow MSCs, adipose-derived MSCs, MSC banking, differentiation studies, and cell manufacturing process development.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cdiv class=\"pdp-table-responsive\"\u003e\n\u003ctable class=\"pdp-table\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eXR MSC Max Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eXRhMSCM-500\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eProduct Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman mesenchymal stem cell serum-free culture kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBasal Medium\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e475 mL × 1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSupplement\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e25 mL × 1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTotal Prepared Volume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e500 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eCulture Format\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eSerum-free and animal component-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended Cell Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman mesenchymal stem cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSupported Tissue Sources\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUmbilical cord, bone marrow, adipose tissue, and other evaluated human MSC sources\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended Seeding Density\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e8,000–10,000 cells\/cm²\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSurface Coating\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNo precoating required under the recommended culture conditions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003epH\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eBasal medium: 7.2–7.4\u003cbr\u003eSupplement: 7.2–7.4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eEndotoxin\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eBasal medium: ≤0.1 EU\/mL\u003cbr\u003eSupplement: ≤0.1 EU\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eFiltration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026lt;0.2 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBacteria Detection\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNot detected\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eFungi Detection\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNot detected\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eBasal medium: 2–8°C\u003cbr\u003eSupplement: −20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eShelf Life\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGrade\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFor Research Use Only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eSerum-free human MSC culture:\u003c\/strong\u003e Provides an alternative to conventional αMEM plus human platelet lysate culture systems.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eAnimal component-free formulation:\u003c\/strong\u003e Designed to reduce reliance on animal-derived culture components and support more standardized MSC workflows.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eOptimized two-component kit:\u003c\/strong\u003e Includes a basal medium and dedicated supplement developed specifically for human mesenchymal stem cell expansion.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eEfficient MSC proliferation:\u003c\/strong\u003e Supports repeated expansion over 72-hour culture intervals under the evaluated conditions.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eLong-term MSC culture:\u003c\/strong\u003e Developed to support stable serial passage while maintaining multipotent differentiation capacity.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eMultiple tissue sources:\u003c\/strong\u003e Suitable for MSCs derived from human umbilical cord, bone marrow, adipose tissue, and other compatible sources.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eNo precoating required:\u003c\/strong\u003e Human MSCs can be seeded directly into standard culture vessels under the recommended workflow.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eLow endotoxin specification:\u003c\/strong\u003e Both the basal medium and supplement have an endotoxin specification of ≤0.1 EU\/mL.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eResearch workflow flexibility:\u003c\/strong\u003e Suitable for MSC isolation, routine propagation, cell banking, differentiation studies, and upstream process research.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eRecommended Culture Workflow\u003c\/h2\u003e\n\u003cp\u003ePrepare the complete MSC expansion medium by combining the XR MSC Max basal medium with the supplied supplement using aseptic technique. Follow the laboratory’s validated preparation and handling procedures when mixing, aliquoting, and storing complete medium.\u003c\/p\u003e\n\u003cp\u003eFor routine passaging, use a gentle stem cell dissociation enzyme and seed the harvested human MSCs at approximately \u003cstrong\u003e8,000–10,000 cells\/cm²\u003c\/strong\u003e. Under the evaluated workflow, cells were cultured for approximately 72 hours per passage. Culture vessels did not require precoating under the recommended conditions.\u003c\/p\u003e\n\u003cp\u003eCell morphology, attachment, confluence, and proliferation rate should be monitored during culture. Seeding density, passage timing, dissociation conditions, and medium volume may require optimization for different donors, tissue sources, culture vessels, and experimental objectives.\u003c\/p\u003e\n\u003ch2\u003eExpansion Performance\u003c\/h2\u003e\n\u003ch3\u003eSerial Passage of Human Mesenchymal Stem Cells\u003c\/h3\u003e\n\u003cp\u003eXR MSC Max Medium was evaluated during ten consecutive passages of human MSC culture. Cells were seeded at 8,000 cells\/cm² during passages 1–7 and at 10,000 cells\/cm² during passages 8–10. Each passage was cultured for approximately 72 hours.\u003c\/p\u003e\n\u003cdiv class=\"pdp-table-responsive\"\u003e\n\u003ctable class=\"pdp-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003ePassage\u003c\/th\u003e\n\u003cth\u003eSeeding Density\u003cbr\u003e(cells\/cm²)\u003c\/th\u003e\n\u003cth\u003eCulture Time\u003cbr\u003e(hours)\u003c\/th\u003e\n\u003cth\u003eHarvested Cells\u003cbr\u003e(×10⁶)\u003c\/th\u003e\n\u003cth\u003eExpansion Fold\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e01\u003c\/td\u003e\n\u003ctd\u003e8,000\u003c\/td\u003e\n\u003ctd\u003e72\u003c\/td\u003e\n\u003ctd\u003e2.57\u003c\/td\u003e\n\u003ctd\u003e13.3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e02\u003c\/td\u003e\n\u003ctd\u003e8,000\u003c\/td\u003e\n\u003ctd\u003e72\u003c\/td\u003e\n\u003ctd\u003e2.19\u003c\/td\u003e\n\u003ctd\u003e10.9\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e03\u003c\/td\u003e\n\u003ctd\u003e8,000\u003c\/td\u003e\n\u003ctd\u003e72\u003c\/td\u003e\n\u003ctd\u003e2.10\u003c\/td\u003e\n\u003ctd\u003e10.5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e04\u003c\/td\u003e\n\u003ctd\u003e8,000\u003c\/td\u003e\n\u003ctd\u003e72\u003c\/td\u003e\n\u003ctd\u003e2.03\u003c\/td\u003e\n\u003ctd\u003e11.1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e05\u003c\/td\u003e\n\u003ctd\u003e8,000\u003c\/td\u003e\n\u003ctd\u003e72\u003c\/td\u003e\n\u003ctd\u003e1.95\u003c\/td\u003e\n\u003ctd\u003e9.7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e06\u003c\/td\u003e\n\u003ctd\u003e8,000\u003c\/td\u003e\n\u003ctd\u003e72\u003c\/td\u003e\n\u003ctd\u003e1.74\u003c\/td\u003e\n\u003ctd\u003e10.4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e07\u003c\/td\u003e\n\u003ctd\u003e8,000\u003c\/td\u003e\n\u003ctd\u003e72\u003c\/td\u003e\n\u003ctd\u003e1.75\u003c\/td\u003e\n\u003ctd\u003e9.0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e08\u003c\/td\u003e\n\u003ctd\u003e10,000\u003c\/td\u003e\n\u003ctd\u003e72\u003c\/td\u003e\n\u003ctd\u003e1.99\u003c\/td\u003e\n\u003ctd\u003e8.1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e09\u003c\/td\u003e\n\u003ctd\u003e10,000\u003c\/td\u003e\n\u003ctd\u003e72\u003c\/td\u003e\n\u003ctd\u003e2.09\u003c\/td\u003e\n\u003ctd\u003e8.4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10\u003c\/td\u003e\n\u003ctd\u003e10,000\u003c\/td\u003e\n\u003ctd\u003e72\u003c\/td\u003e\n\u003ctd\u003e1.96\u003c\/td\u003e\n\u003ctd\u003e7.9\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp\u003eAcross the evaluated ten-passage culture period, the measured expansion ranged from approximately 7.9-fold to 13.3-fold per passage. These data are representative of the tested cell source and experimental conditions and should be used as reference performance data rather than a guaranteed result for every MSC line.\u003c\/p\u003e\n\u003c!-- IMAGE 2 POSITION: Serial passage and MSC phenotype data\n       Use the original figure containing the expansion comparison chart\n       and passage 10 MSC flow cytometry histograms.\n\n       Replace PASTE_MSC_EXPANSION_PHENOTYPE_IMAGE_URL_HERE with the Shopify CDN image URL.\n\n       Recommended alt:\n       Human MSC serial expansion performance and passage 10 mesenchymal stem cell phenotype analysis with XR MSC Max medium\n  --\u003e\n\u003cdiv class=\"pdp-img-container\" style=\"text-align: center;\"\u003e\n\u003cimg alt=\"Human MSC serial expansion performance and passage 10 mesenchymal stem cell phenotype analysis with XR MSC Max medium\" height=\"620\" width=\"1000\" loading=\"lazy\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/886a010b-62f3-42ee-bcae-06a741a00aee.png?v=1784014014\" style=\"float: none;\"\u003e\n\u003cp class=\"pdp-img-caption\"\u003eRepresentative human MSC expansion and phenotype data following serial culture in XR MSC Max Medium.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch2\u003eMSC Phenotype and Multipotency\u003c\/h2\u003e\n\u003ch3\u003ePhenotype Maintenance After Serial Culture\u003c\/h3\u003e\n\u003cp\u003eHuman MSCs cultured with XR MSC Max Medium were evaluated by flow cytometry after ten passages. The representative histograms demonstrate phenotype analysis following extended serial expansion in the serum-free culture system.\u003c\/p\u003e\n\u003cp\u003eMarker expression and acceptance criteria should be established according to the laboratory’s validated MSC characterization panel, tissue source, donor characteristics, and intended research application.\u003c\/p\u003e\n\u003ch3\u003eThree-Lineage Differentiation Potential\u003c\/h3\u003e\n\u003cp\u003eFollowing continuous culture in XR MSC Max Medium, the expanded MSCs were subjected to adipogenic, osteogenic, and chondrogenic induction. Representative staining results showed that the cultured cells retained the ability to undergo three-lineage mesenchymal differentiation under the evaluated conditions.\u003c\/p\u003e\n\u003c!-- IMAGE 3 POSITION: MSC differentiation and morphology\n     Use the original figure containing:\n     1. Adipogenic differentiation\n     2. Osteogenic differentiation\n     3. Chondrogenic differentiation\n     4. P1, P5 and P10 cell morphology\n--\u003e\n\u003cdiv class=\"pdp-img-container\" style=\"text-align: center;\"\u003e\n\u003cimg loading=\"lazy\" height=\"520\" width=\"1000\" alt=\"Adipogenic osteogenic and chondrogenic differentiation with P1 P5 and P10 human MSC morphology after XR MSC Max culture\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/2026-07-14_151658_003.png?v=1784013440\" style=\"float: none;\"\u003e\n\u003cp class=\"pdp-img-caption\"\u003eRepresentative adipogenic, osteogenic, chondrogenic differentiation and serial morphology (P1, P5 and P10) of human MSCs cultured with XR MSC Max Medium.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eXR MSC Max Kit is designed for human mesenchymal stem cell research requiring a consistent serum-free expansion environment. Its basal medium and dedicated supplement support workflows ranging from primary MSC culture to serial propagation and downstream differentiation studies.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eHuman MSC isolation and primary culture:\u003c\/strong\u003e Supports early-stage culture workflows for MSCs isolated from compatible human tissue sources.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eUmbilical cord MSC culture:\u003c\/strong\u003e Suitable for expansion studies involving human umbilical cord-derived mesenchymal stem cells.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBone marrow MSC culture:\u003c\/strong\u003e Can be used as a serum-free MSC expansion medium for human bone marrow-derived MSC research.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eAdipose-derived MSC culture:\u003c\/strong\u003e Supports propagation studies using human adipose-derived mesenchymal stem or stromal cells.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eLong-term MSC expansion:\u003c\/strong\u003e Designed for repeated passage workflows requiring sustained human MSC proliferation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eMSC banking:\u003c\/strong\u003e Suitable for generating expanded cell populations before cryopreservation and research cell bank establishment.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eMSC phenotype characterization:\u003c\/strong\u003e Supports cell preparation for flow cytometry and other mesenchymal stem cell characterization assays.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eMSC differentiation research:\u003c\/strong\u003e Suitable for generating expanded MSC populations for adipogenic, osteogenic, and chondrogenic differentiation studies.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eRegenerative medicine research:\u003c\/strong\u003e Supports research workflows involving mesenchymal stem cell biology, tissue repair, and tissue engineering.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCell therapy process development:\u003c\/strong\u003e May be used in research-stage evaluation of serum-free and animal component-free MSC expansion processes.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eExtracellular vesicle research:\u003c\/strong\u003e Can support upstream MSC expansion studies where researchers require a serum-free culture background before downstream extracellular vesicle analysis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eMSC manufacturing research:\u003c\/strong\u003e Suitable for laboratory-scale studies investigating seeding density, passage timing, harvest yield, and culture process consistency.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eWhy Choose XR MSC Max Kit?\u003c\/h2\u003e\n\u003cp\u003eSerum- and platelet lysate-supplemented MSC media can provide effective growth, but differences between raw-material lots may affect proliferation, cell morphology, and downstream experimental consistency. These variables become increasingly important during long-term culture or when comparing MSCs from different donors and tissue sources.\u003c\/p\u003e\n\u003cp\u003eXR MSC Max Kit provides a serum-free and animal component-free human MSC culture system developed to reduce dependence on undefined serum components. The dedicated basal medium and supplement are optimized as a complete \u003cstrong\u003emesenchymal stem cell expansion medium\u003c\/strong\u003e for routine propagation, serial passage, and differentiation research.\u003c\/p\u003e\n\u003cp\u003eThe evaluated workflow combines a recommended seeding density of 8,000–10,000 cells\/cm² with gentle enzymatic dissociation and no precoating requirement. This streamlined approach can help laboratories establish a more standardized human MSC culture process while retaining flexibility for donor-specific and application-specific optimization.\u003c\/p\u003e\n\u003ch2\u003eImportant Culture Considerations\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003ePerformance may vary according to donor, tissue source, isolation method, passage history, initial cell quality, and culture vessel.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eConfirm cell identity and MSC phenotype using an appropriate laboratory-validated characterization panel.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eOptimize seeding density when transferring cells from serum-containing or platelet lysate-containing culture systems.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAvoid excessive dissociation time or harsh mechanical handling that may reduce cell attachment and viability.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eMonitor cultures regularly and determine passage timing according to cell morphology and confluence rather than culture time alone.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eValidate downstream differentiation, extracellular vesicle, or molecular analysis workflows using the specific MSC source and experimental design.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is XR MSC Max Kit used for?\u003c\/summary\u003e\n\u003cp\u003eXR MSC Max Kit is a human mesenchymal stem cell serum-free culture kit designed for MSC isolation, routine expansion, continuous passage, cell banking, phenotype analysis, and differentiation research.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eIs XR MSC Max a serum-free MSC medium?\u003c\/summary\u003e\n\u003cp\u003eYes. XR MSC Max is a serum-free human MSC culture system supplied as a basal medium and dedicated supplement. It does not require additional serum under the recommended culture conditions.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eIs this human MSC medium animal component-free?\u003c\/summary\u003e\n\u003cp\u003eYes. The formulation is described as animal component-free, making it suitable for research workflows seeking to reduce reliance on animal-derived culture components.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhich types of human mesenchymal stem cells can be cultured?\u003c\/summary\u003e\n\u003cp\u003eThe medium is suitable for MSCs from multiple human tissue sources, including umbilical cord, bone marrow, and adipose tissue. Culture conditions should be optimized for each donor and cell source.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan this medium be used for umbilical cord MSC expansion?\u003c\/summary\u003e\n\u003cp\u003eYes. XR MSC Max Kit can be used as a serum-free medium for human umbilical cord MSC culture and serial expansion research.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan XR MSC Max be used for bone marrow or adipose-derived MSCs?\u003c\/summary\u003e\n\u003cp\u003eYes. The product is intended for multiple MSC sources, including human bone marrow-derived MSCs and adipose-derived MSCs. Expansion performance may differ between donors and tissue sources.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat seeding density is recommended for human MSC culture?\u003c\/summary\u003e\n\u003cp\u003eThe evaluated workflow used a seeding density of approximately 8,000–10,000 cells\/cm². Researchers should optimize the density according to cell source, passage number, vessel format, and experimental objective.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eDoes the culture vessel require precoating?\u003c\/summary\u003e\n\u003cp\u003eNo precoating was required under the recommended XR MSC Max culture conditions. Attachment should still be verified when using a new MSC source or culture vessel.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eDoes XR MSC Max support long-term MSC expansion?\u003c\/summary\u003e\n\u003cp\u003eThe product was developed to support stable serial passage and is described as supporting culture beyond 20 passages while maintaining multilineage differentiation potential. Representative expansion data are provided for ten consecutive passages.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat expansion rate was observed during testing?\u003c\/summary\u003e\n\u003cp\u003eDuring the reported ten-passage evaluation, expansion ranged from approximately 7.9-fold to 13.3-fold per 72-hour passage. Results are reference data and may vary between MSC sources and laboratory conditions.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eDoes the medium maintain MSC multipotency?\u003c\/summary\u003e\n\u003cp\u003eRepresentative results showed adipogenic, osteogenic, and chondrogenic differentiation after continuous culture, indicating maintenance of three-lineage differentiation potential under the evaluated conditions.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan this MSC expansion medium be used for extracellular vesicle research?\u003c\/summary\u003e\n\u003cp\u003eThe serum-free formulation can support upstream MSC expansion studies where a reduced-serum-background culture system is desirable. Researchers should independently validate collection timing, medium conditioning, isolation methods, and analytical controls for extracellular vesicle studies.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow should XR MSC Max Kit be stored?\u003c\/summary\u003e\n\u003cp\u003eStore the basal medium at 2–8°C and the supplement at −20°C. Both components have a stated shelf life of 12 months when stored as directed.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eIs XR MSC Max Kit intended for clinical use?\u003c\/summary\u003e\n\u003cp\u003eNo. XR MSC Max Kit is supplied for research use only and is not intended for diagnostic, therapeutic, or direct clinical use.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cp\u003e\u003cem\u003eFor Research Use Only. Not intended for diagnostic, therapeutic, or clinical use.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003cscript type=\"application\/ld+json\"\u003e\n{\n  \"@context\": \"https:\/\/schema.org\",\n  \"@type\": \"Product\",\n  \"name\": \"XR MSC Max Kit\",\n  \"sku\": \"XRhMSCM-500\",\n  \"mpn\": \"XRhMSCM-500\",\n  \"brand\": {\n    \"@type\": \"Brand\",\n    \"name\": \"XR\"\n  },\n  \"description\": \"A serum-free, animal component-free human mesenchymal stem cell culture kit designed for MSC isolation, serial expansion, phenotype maintenance, and multilineage differentiation research.\",\n  \"category\": \"Human Mesenchymal Stem Cell Culture Medium\",\n  \"additionalProperty\": [\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Product Type\",\n      \"value\": \"Human mesenchymal stem cell serum-free culture kit\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Basal Medium\",\n      \"value\": \"475 mL\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Supplement\",\n      \"value\": \"25 mL\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Total Prepared Volume\",\n      \"value\": \"500 mL\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Culture Format\",\n      \"value\": \"Serum-free and animal component-free\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Recommended Seeding Density\",\n      \"value\": \"8,000–10,000 cells\/cm²\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Endotoxin\",\n      \"value\": \"≤0.1 EU\/mL\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Filtration\",\n      \"value\": \"\u003c0.2 μm\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Basal Medium Storage\",\n      \"value\": \"2–8°C\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Supplement Storage\",\n      \"value\": \"−20°C\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Shelf Life\",\n      \"value\": \"12 months\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Grade\",\n      \"value\": \"For Research Use Only\"\n    }\n  ]\n}\n\u003c\/script\u003e \u003cscript type=\"application\/ld+json\"\u003e\n{\n  \"@context\": \"https:\/\/schema.org\",\n  \"@type\": \"FAQPage\",\n  \"mainEntity\": [\n    {\n      \"@type\": \"Question\",\n      \"name\": \"What is XR MSC Max Kit used for?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"XR MSC Max Kit is a human mesenchymal stem cell serum-free culture kit designed for MSC isolation, routine expansion, continuous passage, cell banking, phenotype analysis, and differentiation research.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"Is XR MSC Max a serum-free MSC medium?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"Yes. XR MSC Max is a serum-free human MSC culture system supplied as a basal medium and dedicated supplement. It does not require additional serum under the recommended culture conditions.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"Is this human MSC medium animal component-free?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"Yes. The formulation is described as animal component-free and is suitable for research workflows seeking to reduce reliance on animal-derived culture components.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"Which types of human mesenchymal stem cells can be cultured?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"The medium is suitable for MSCs from multiple human tissue sources, including umbilical cord, bone marrow, and adipose tissue. Culture conditions should be optimized for each donor and cell source.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"What seeding density is recommended for human MSC culture?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"The evaluated workflow used a seeding density of approximately 8,000 to 10,000 cells per square centimeter. Researchers should optimize the density according to cell source, passage number, vessel format, and experimental objective.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"Does the culture vessel require precoating?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"No precoating was required under the recommended XR MSC Max culture conditions. Attachment should still be verified when using a new MSC source or culture vessel.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"Does XR MSC Max support long-term MSC expansion?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"The product was developed to support stable serial passage and is described as supporting culture beyond 20 passages while maintaining multilineage differentiation potential. Representative expansion data are provided for ten consecutive passages.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"Does the medium maintain MSC multipotency?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"Representative results showed adipogenic, osteogenic, and chondrogenic differentiation after continuous culture, indicating maintenance of three-lineage differentiation potential under the evaluated conditions.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"How should XR MSC Max Kit be stored?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"Store the basal medium at 2 to 8 degrees Celsius and the supplement at minus 20 degrees Celsius. Both components have a stated shelf life of 12 months when stored as directed.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"Is XR MSC Max Kit intended for clinical use?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"No. XR MSC Max Kit is supplied for research use only and is not intended for diagnostic, therapeutic, or direct clinical use.\"\n      }\n    }\n  ]\n}\n\u003c\/script\u003e\u003c\/p\u003e","brand":"XRbio","offers":[{"title":"1Kit","offer_id":52798381129909,"sku":"XRhMSCM-500","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/2_420105ab-d889-40de-a789-2633b1b5874e.jpg?v=1784013484","url":"https:\/\/biofargo.com\/products\/xr-msc-max-kit-human-msc-serum-free-medium-kit","provider":"Biofargo","version":"1.0","type":"link"}