Name: XR Human MSC Expansion Medium Kit (Chemically Defined)
Brand: XR

Can XR MSC Expansion Medium support MSC differentiation?

Yes. XR MSC-CD Medium supports maintenance of MSC multipotency and trilineage differentiation research including osteogenic, chondrogenic, and adipogenic differentiation.

What cells are compatible with XR Human MSC Expansion Medium?

XR Human MSC Expansion Medium is suitable for human adipose-derived MSCs, bone marrow-derived MSCs, and umbilical cord-derived MSCs.

Description

Maintaining reliable expansion performance of human mesenchymal stem cells (MSCs) can be challenging when traditional serum-containing culture systems introduce batch variation, undefined components, and inconsistent growth conditions. Researchers working with adipose-derived MSCs, bone marrow MSCs, or umbilical cord MSCs often require a more standardized culture environment to support reproducible cell expansion and downstream functional studies.

XR Human MSC Expansion Medium Kit (Chemically Defined) is a chemically defined human MSC culture medium designed for the isolation, expansion, and maintenance of human mesenchymal stem cells. The formulation contains no human serum, no animal-derived components, and no platelet lysate or AB plasma components, providing a controlled environment for consistent MSC culture workflows.

This serum-free MSC expansion medium supports long-term human MSC expansion while maintaining key biological characteristics, including MSC phenotype and multipotency. XR MSC-CD Medium is suitable for researchers requiring a defined MSC culture system for routine expansion, differentiation studies, exosome-related research, and advanced cell culture applications.

In addition to supporting conventional 2D MSC culture, XR Human MSC Expansion Medium Kit can be combined with microcarrier-based workflows for 3D MSC culture applications, providing flexibility for researchers developing scalable MSC expansion strategies.

Specifications

Product Name	XR Human MSC Expansion Medium Kit (Chemically Defined)
Product Type	Human mesenchymal stem cell expansion medium
Product Components	MSC-CD Expansion Medium and MSC-CD Coating Solution
Application	Human MSC isolation, expansion, maintenance, and differentiation research
Compatible Cells	Human adipose-derived MSCs, bone marrow-derived MSCs, and umbilical cord-derived MSCs
MSC-CD Expansion Medium	500 mL × 1 bottle
MSC-CD Coating Solution	1 mL × 1 vial
Medium pH	7.2–7.4
Coating Solution pH	6.8–7.2
Filtration	<0.2 μm filtered
Endotoxin Level	<0.125 EU/mL
Bacteria Detection	Not detected
Fungi Detection	Not detected
Storage	-20°C
Shelf Life	12 months
Research Grade	For Research Use Only (RUO)

Features

Chemically Defined MSC Culture System — Provides a controlled MSC expansion environment without human serum, animal-derived components, platelet lysate, or AB plasma components.
Serum-Free Human MSC Expansion Medium — Supports expansion of human mesenchymal stem cells while reducing variability associated with traditional serum-containing culture systems.
Supports Multiple Human MSC Sources — Suitable for adipose-derived MSC, bone marrow MSC, and umbilical cord MSC culture workflows.
Maintains MSC Biological Characteristics — Designed to preserve MSC phenotype and multipotent differentiation capability during expansion.
Low Exosome Background Culture Environment — The exosome-free formulation provides a cleaner platform for MSC-derived exosome collection and downstream analysis.
Compatible with 3D MSC Expansion Workflows — Can be combined with microcarrier-based culture systems for scalable MSC culture applications.

Performance Data

Long-Term Human MSC Expansion Performance

XR Human MSC Expansion Medium Kit (Chemically Defined) was evaluated for continuous expansion performance using human mesenchymal stem cells cultured in T175 vessels. Cells were seeded at a consistent density of 6,000 cells/cm² and monitored through 10 sequential passages to evaluate expansion capability during extended culture.

The results demonstrated that XR MSC-CD Medium supported sustained human MSC proliferation throughout serial passaging. Cells maintained expansion capacity from early passages to later passages, providing a reliable culture environment for long-term MSC expansion workflows.

Passage	Seeding Density (cells/cm²)	Harvested Cell Number	Expansion Fold
P1	6000	1.12 × 10⁷	10.6
P2	6000	1.29 × 10⁷	12.3
P3	6000	1.50 × 10⁷	14.3
P4	6000	1.56 × 10⁷	14.8
P5	6000	1.38 × 10⁷	13.2
P6	6000	1.25 × 10⁷	11.9
P7	6000	1.06 × 10⁷	10.1
P8	6000	1.00 × 10⁷	9.5
P9	6000	9.56 × 10⁶	9.1
P10	6000	9.11 × 10⁶	8.7

Representative continuous passage results demonstrate stable expansion performance of human MSCs cultured with XR MSC-CD Medium. The data support its use as a human MSC expansion medium for long-term culture and research workflows requiring consistent cell growth.

Maintenance of MSC Multipotency After Expansion

Maintaining differentiation potential after expansion is an important consideration for human mesenchymal stem cell culture. XR Human MSC Expansion Medium was evaluated for post-expansion differentiation capability through induction toward three mesenchymal lineages.

Expanded MSCs cultured with XR MSC-CD Medium retained the ability to differentiate into osteogenic, chondrogenic, and adipogenic lineages, demonstrating preservation of MSC multipotency after culture expansion.

Osteogenic chondrogenic and adipogenic trilineage differentiation of human MSCs expanded in chemically defined MSC medium

Representative differentiation results include osteogenic, chondrogenic, and adipogenic induction after MSC expansion, supporting the ability of XR MSC-CD Medium to maintain human MSC functional characteristics.

Human MSC Surface Marker Characterization

Flow cytometry analysis was performed to characterize human MSC identity after expansion using XR MSC-CD Medium. Common MSC-associated markers including CD73, CD90, and CD105 were evaluated together with CD34 analysis.

Flow cytometry characterization of human MSC markers CD34 CD73 CD105 and CD90 after culture in MSC expansion medium

Flow cytometry results demonstrate MSC marker characterization following expansion, supporting the suitability of XR MSC-CD Medium for human mesenchymal stem cell culture and phenotype analysis.

Applications

XR Human MSC Expansion Medium Kit (Chemically Defined) is designed for researchers requiring a standardized human MSC culture environment for expansion, characterization, differentiation, and advanced cell biology studies.

Human MSC Expansion and Maintenance — Supports routine expansion of human mesenchymal stem cells from adipose tissue, bone marrow, and umbilical cord sources.
Serum-Free MSC Culture Workflows — Suitable for researchers seeking serum-free and chemically defined MSC culture conditions with reduced variability.
Human Mesenchymal Stem Cell Characterization — Supports MSC phenotype evaluation using commonly analyzed surface markers including CD73, CD90, CD105, and CD34.
MSC Differentiation Research — Suitable for studies involving osteogenic, chondrogenic, and adipogenic differentiation of expanded MSC populations.
MSC Exosome Research — The low exosome background formulation provides a cleaner culture environment for MSC-derived exosome collection and downstream analysis.
3D MSC Culture and Scale-Up Development — Compatible with microcarrier-based culture strategies for researchers developing expanded MSC production workflows.
Regenerative Medicine Research — Supports basic research involving human MSC biology, cell expansion, and differentiation studies.
Stem Cell Banking and Research Cell Preparation — Provides a defined culture system for preparation and maintenance of research-grade MSC populations.

Why Choose XR Human MSC Expansion Medium?

Human mesenchymal stem cell research often requires more than simple cell proliferation. Variability from undefined culture components, inconsistent serum sources, and external biological factors may influence MSC growth characteristics and downstream experimental interpretation.

XR Human MSC Expansion Medium Kit provides a chemically defined MSC culture environment without human serum, animal-derived components, platelet lysate, or AB plasma components. This controlled formulation helps researchers establish more consistent MSC expansion workflows while maintaining key MSC characteristics including proliferation ability, phenotype, and trilineage differentiation potential.

For studies involving MSC-derived extracellular vesicles, XR MSC-CD Medium provides an exosome-free culture platform designed to minimize background interference from external exosome sources, supporting cleaner MSC exosome research applications.

FAQ

What is XR Human MSC Expansion Medium Kit (Chemically Defined)?

XR Human MSC Expansion Medium Kit is a chemically defined human mesenchymal stem cell culture medium designed for the isolation, expansion, and maintenance of human MSCs. The formulation does not contain human serum, animal-derived components, platelet lysate, or AB plasma components, providing a controlled environment for MSC expansion research.

What cells can be cultured with XR Human MSC Expansion Medium?

XR MSC Expansion Medium is designed for human mesenchymal stem cells including adipose-derived MSCs, bone marrow-derived MSCs, and umbilical cord-derived MSCs. It supports routine MSC culture, expansion, and characterization workflows.

Is XR MSC Expansion Medium serum-free?

Yes. XR Human MSC Expansion Medium Kit is a serum-free MSC culture medium. It is formulated without human serum and without animal-derived components, helping reduce variability associated with traditional serum-containing culture systems.

What is the advantage of a chemically defined MSC medium?

A chemically defined MSC medium provides a more controlled culture environment compared with undefined serum-based systems. By reducing variation from external biological components, researchers can achieve more consistent human MSC expansion conditions for downstream studies.

Does XR Human MSC Expansion Medium support MSC differentiation?

Yes. XR MSC-CD Medium is designed to maintain human MSC biological characteristics and supports trilineage differentiation research, including osteogenic, chondrogenic, and adipogenic differentiation after expansion.

Can XR MSC Expansion Medium be used for MSC exosome research?

Yes. XR MSC-CD Medium is an exosome-free culture medium with low background exosome interference. It provides a cleaner culture environment for collecting MSC-derived exosomes and performing downstream extracellular vesicle research.

Is XR MSC Medium suitable for long-term human MSC expansion?

Yes. XR Human MSC Expansion Medium was evaluated through continuous ten-passage culture experiments. The expansion data demonstrate sustained MSC proliferation capability during extended culture conditions.

Can XR Human MSC Expansion Medium be used for 3D MSC culture?

Yes. XR MSC-CD Medium can be combined with microcarrier-based culture approaches and is suitable for research workflows involving three-dimensional MSC culture and scalable expansion strategies.

What MSC markers were evaluated after culture with XR MSC Medium?

Human MSC characterization was performed using flow cytometry analysis of commonly evaluated MSC markers, including CD73, CD90, CD105, and CD34.

What are the storage conditions for XR Human MSC Expansion Medium Kit?

XR MSC-CD Expansion Medium and MSC-CD Coating Solution should be stored at -20°C. The recommended shelf life is 12 months under proper storage conditions.

What is the difference between XR MSC Medium and traditional serum-containing MSC media?

Traditional MSC culture media commonly rely on serum supplements that may introduce biological variability. XR MSC Medium provides a chemically defined and serum-free culture system designed to improve consistency for human MSC expansion and functional studies.

Is XR Human MSC Expansion Medium intended for clinical use?

No. XR Human MSC Expansion Medium Kit is intended for research use only (RUO). It is designed for laboratory research applications involving human mesenchymal stem cell culture and characterization.

XR MSC CD Kit – Human MSC Chemically-Defined Medium Kit