{"product_id":"xr-ipsc-derived-liver-organoid-differentiation-kit","title":"XR iPSC-Derived Liver Organoid Differentiation Kit","description":"\u003c!--\nSEO TITLE:\nLiver Organoid Differentiation Kit | Human iPSC\n\nMETA DESCRIPTION:\nDifferentiate human iPSCs into 3D liver organoids with a staged, chemically defined A–F medium workflow for liver development and disease-model research.\n\nRECOMMENDED URL:\n\/products\/liver-organoid-differentiation-kit\n\nPRIMARY KEYWORDS:\nLiver Organoid Differentiation Kit\nHuman iPSC Liver Organoid\nHuman Liver Organoid\nLiver Organoid Differentiation\nHepatic Organoid Differentiation\n\nSECONDARY KEYWORDS:\niPSC Liver Differentiation\nhPSC Liver Organoid\nLiver Organoid Culture Medium\nHepatic Differentiation Medium\n3D Liver Organoid Culture\nHuman Hepatic Organoid\nLiver Development Model\nLiver Disease Modeling\nLiver Organoid Drug Screening\nChemically Defined Differentiation Medium\n--\u003e\n\u003cstyle\u003e\n  \/* 现代专业风全局样式 *\/\n  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class=\"pdp-container\"\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eGenerating human liver organoids from induced pluripotent stem cells requires careful coordination of cell confluence, stage-specific differentiation signals, aggregate size, extracellular matrix embedding, and medium changes. Variability at any of these steps can affect organoid formation, morphology, and the reproducibility of downstream liver development or disease-modeling studies.\u003c\/p\u003e\n\u003cp\u003eXR Liver Organoid Differentiation Kit is a chemically defined, stage-specific differentiation system designed to guide human pluripotent stem cells through sequential hepatic lineage induction and three-dimensional liver organoid formation. The workflow uses six designated media, supplied as Liver Organoid Differentiation Media A through F, to support a structured differentiation process from adherent hPSC culture to matrix-embedded human liver organoids.\u003c\/p\u003e\n\u003cp\u003eThe protocol begins with human iPSCs at approximately 80% confluence in a 6-well plate. Cells are exposed sequentially to differentiation media A, B, C, and D before being collected as aggregates, adjusted to approximately 100 μm in diameter, and embedded in an organoid culture matrix. Media E and F are then used to support three-dimensional organoid formation and continued maturation through Day 20 and beyond.\u003c\/p\u003e\n\u003cp\u003eThis human iPSC liver organoid differentiation kit provides a standardized workflow for laboratories studying human liver development, hepatic lineage differentiation, liver disease modeling, and liver organoid-based drug screening.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cdiv class=\"pdp-table-responsive\"\u003e\n\u003ctable class=\"pdp-table\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eXR Liver Organoid Differentiation Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eXR-iLiverOrg-Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eProduct Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman pluripotent stem cell liver organoid differentiation kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStarting Cell Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman pluripotent stem cells, including human induced pluripotent stem cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eCulture Format\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAdherent differentiation followed by 3D matrix-embedded organoid culture\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDifferentiation Format\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eSequential six-medium workflow using Media A–F\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStarting Confluence\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eApproximately 80% iPSC confluence in a 6-well plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended Aggregate Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eApproximately 100 μm in diameter before matrix embedding\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003e3D Culture Plate\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e24-well plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix Dome Volume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e100 μL per well\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix Polymerization\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e30 minutes at 37°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDifferentiation Period\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eDay 0 through Day 20+\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eObserved Markers\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHNF4α and albumin (ALB)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eIntended Use\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eResearch use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003cdiv class=\"pdp-table-responsive\"\u003e\n\u003ctable class=\"pdp-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eVolume\u003c\/th\u003e\n\u003cth\u003eWorkflow Stage\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eLiver Organoid Differentiation Medium A\u003c\/td\u003e\n\u003ctd\u003e6 mL\u003c\/td\u003e\n\u003ctd\u003eDay 0 initial differentiation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLiver Organoid Differentiation Medium B\u003c\/td\u003e\n\u003ctd\u003e6 mL\u003c\/td\u003e\n\u003ctd\u003eDay 1 differentiation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLiver Organoid Differentiation Medium C\u003c\/td\u003e\n\u003ctd\u003e6 mL\u003c\/td\u003e\n\u003ctd\u003eDay 2 differentiation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLiver Organoid Differentiation Medium D\u003c\/td\u003e\n\u003ctd\u003e12 mL\u003c\/td\u003e\n\u003ctd\u003eDay 3–5 hepatic lineage differentiation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLiver Organoid Differentiation Medium E\u003c\/td\u003e\n\u003ctd\u003e20 mL\u003c\/td\u003e\n\u003ctd\u003eDay 6–10 matrix-embedded organoid formation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLiver Organoid Differentiation Medium F\u003c\/td\u003e\n\u003ctd\u003e50 mL\u003c\/td\u003e\n\u003ctd\u003eDay 11–20+ liver organoid maturation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"pdp-note\"\u003e\n\u003cp\u003e\u003cstrong\u003eImportant:\u003c\/strong\u003e The source protocol requires an organoid culture matrix for three-dimensional embedding. The matrix is not listed among the supplied A–F differentiation media and should be prepared separately according to the laboratory’s validated workflow.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eStage-specific liver organoid differentiation medium system for human pluripotent stem cells\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSix designated media support sequential differentiation from adherent iPSC culture to mature 3D liver organoids\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eChemically defined differentiation system designed to reduce the need for manual preparation of multiple induction formulations\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eStructured Day 0 to Day 20+ workflow for hepatic lineage induction, organoid formation, and maturation\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eCombines two-dimensional early-stage differentiation with matrix-embedded 3D liver organoid culture\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSupports formation of liver organoids displaying HNF4α and albumin expression in the supplied product data\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSuitable for human liver development, hepatic differentiation, disease-modeling, and drug-screening research\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eDefined medium sequence helps laboratories standardize timing and medium transitions across experiments\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eLiver Organoid Differentiation Workflow\u003c\/h2\u003e\n\u003cp\u003eThe following workflow summarizes the protocol supplied with the XR Liver Organoid Differentiation Kit. Culture performance can be influenced by the starting cell line, cell condition, confluence, aggregate uniformity, matrix handling, and laboratory-specific culture practices.\u003c\/p\u003e\n\u003cdiv class=\"pdp-stage\"\u003e\n\u003ch3\u003eDay 0: Initial Differentiation\u003c\/h3\u003e\n\u003cp\u003eBegin differentiation when iPSCs in a 6-well plate reach approximately 80% confluence. Add 2 mL of Liver Organoid Differentiation Medium A per well and culture for 24 hours.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"pdp-stage\"\u003e\n\u003ch3\u003eDay 1: Medium B\u003c\/h3\u003e\n\u003cp\u003eReplace Medium A with 2 mL of Liver Organoid Differentiation Medium B per well.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"pdp-stage\"\u003e\n\u003ch3\u003eDay 2: Medium C\u003c\/h3\u003e\n\u003cp\u003eReplace Medium B with 2 mL of Liver Organoid Differentiation Medium C per well.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"pdp-stage\"\u003e\n\u003ch3\u003eDay 3–5: Medium D\u003c\/h3\u003e\n\u003cp\u003eReplace the culture medium with 2 mL of Liver Organoid Differentiation Medium D per well. Change the medium every other day during this stage.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"pdp-stage\"\u003e\n\u003ch3\u003eDay 6–10: 3D Liver Organoid Formation\u003c\/h3\u003e\n\u003cp\u003eGently detach the differentiated cells using a pipette and adjust the resulting cell aggregates to approximately 100 μm in diameter. Collect the aggregates by centrifugation and combine them with an organoid culture matrix according to the required seeding density.\u003c\/p\u003e\n\u003cp\u003eDispense 100 μL of the matrix-cell suspension into the center of each well of a new 24-well plate. Incubate at 37°C for 30 minutes to allow the matrix to polymerize, then gently add 700 μL of Liver Organoid Differentiation Medium E per well. Replace the medium every other day.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"pdp-stage\"\u003e\n\u003ch3\u003eDay 11–20+: Organoid Maturation\u003c\/h3\u003e\n\u003cp\u003eReplace Medium E with Liver Organoid Differentiation Medium F. Continue changing the medium every other day until the liver organoids reach the desired maturation stage.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"pdp-img-container\"\u003e\n\u003cimg alt=\"Human iPSC liver organoid differentiation timeline showing Day 3 Day 6 Day 12 and Day 20 organoid morphology\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_55672ed7-63a4-4cac-a382-70b749acf064.jpg?v=1784008769\"\u003e\n\u003cp class=\"pdp-img-caption\"\u003eHuman iPSC liver organoid differentiation timeline showing Day 3 Day 6 Day 12 and Day 20 organoid morphology\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003ch3\u003eMorphological Development During Differentiation\u003c\/h3\u003e\n\u003cp\u003eThe supplied product data show progressive changes during the differentiation workflow. Early-stage cultures are shown at Day 3 and Day 6, followed by the appearance of multiple three-dimensional organoid structures at Day 12. By Day 20, the cultures display numerous rounded liver organoid structures distributed throughout the matrix.\u003c\/p\u003e\n\u003cp\u003eThe illustrated workflow is organized into sequential phases consisting of iPSC culture, endoderm differentiation, a prehepatic stage, and liver organoid maturation. These observations provide visual evidence of organoid formation under the reported differentiation conditions but should not be interpreted as a guaranteed outcome for every human pluripotent stem cell line.\u003c\/p\u003e\n\u003ch3\u003eHepatic Marker Expression\u003c\/h3\u003e\n\u003cp\u003eImmunofluorescence images supplied with the product show expression of hepatocyte nuclear factor 4 alpha (HNF4α) and albumin (ALB) in differentiated organoid cultures. HNF4α is commonly evaluated in hepatic lineage studies, while albumin expression is used as an indicator of hepatocyte-associated differentiation.\u003c\/p\u003e\n\u003c!-- IMAGE 3 POSITION: HNF4α and ALB immunofluorescence\n     Replace the src below with your uploaded image URL.\n\n     Recommended alt:\n     HNF4 alpha and albumin immunofluorescence in human iPSC derived liver organoids\n--\u003e\n\u003cdiv class=\"pdp-img-container\" style=\"text-align: center;\"\u003e\n\u003cimg alt=\"HNF4 alpha and albumin immunofluorescence in human iPSC derived liver organoids\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/3_394d5c3b-8f85-4054-be10-1ca7139e5ae8.jpg?v=1784008769\" style=\"float: none;\"\u003e\n\u003cp class=\"pdp-img-caption\"\u003eHNF4 alpha and albumin immunofluorescence in human iPSC derived liver organoids\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eHuman iPSC-derived liver organoids provide a three-dimensional experimental platform for investigating hepatic differentiation and organ development in a controlled in vitro environment. The XR Liver Organoid Differentiation Kit is designed for research workflows that require a standardized transition from pluripotent stem cells to matrix-embedded hepatic organoid cultures.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eHuman liver development research:\u003c\/strong\u003e Study sequential changes associated with endoderm induction, hepatic lineage specification, liver organoid formation, and continued maturation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eHuman iPSC liver differentiation:\u003c\/strong\u003e Generate hepatic organoid cultures from human induced pluripotent stem cells using a stage-specific A–F medium workflow.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003e3D liver organoid culture:\u003c\/strong\u003e Establish matrix-embedded hepatic organoids for studies requiring three-dimensional cell organization rather than conventional monolayer culture alone.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eLiver disease modeling:\u003c\/strong\u003e Develop human iPSC-based liver organoid models for investigating disease-associated cellular and developmental phenotypes.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eDrug screening research:\u003c\/strong\u003e Use human liver organoids as an in vitro research platform for evaluating compound responses under experimentally validated conditions.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eLiver toxicity studies:\u003c\/strong\u003e Support exploratory assessment of treatment-associated effects in human hepatic organoid models after appropriate assay qualification.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eHepatic marker analysis:\u003c\/strong\u003e Evaluate differentiation-associated markers such as HNF4α and albumin using immunofluorescence or other validated analytical methods.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eStem cell differentiation research:\u003c\/strong\u003e Investigate how human pluripotent stem cells transition through endodermal and hepatic lineage stages in vitro.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eOrganoid protocol development:\u003c\/strong\u003e Use the supplied workflow as a foundation for laboratory-specific optimization of seeding density, aggregate handling, matrix conditions, and maturation duration.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eComparative liver model studies:\u003c\/strong\u003e Compare organoid morphology or marker expression among different human iPSC lines under controlled culture conditions.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eWhy Use a Stage-Specific Liver Organoid Differentiation System?\u003c\/h2\u003e\n\u003cp\u003eHuman liver organoid generation is a multistep process rather than a single-medium culture procedure. Early differentiation stages require coordinated changes in signaling conditions, while later stages depend on consistent aggregate preparation, extracellular matrix embedding, and three-dimensional maturation.\u003c\/p\u003e\n\u003cp\u003eThe XR Liver Organoid Differentiation Kit organizes these requirements into six designated media with defined transition points. This format can help reduce preparation complexity and provide a more consistent framework for human iPSC liver differentiation experiments. The workflow also separates early adherent differentiation from later 3D organoid culture, allowing researchers to monitor morphology at key stages before and after matrix embedding.\u003c\/p\u003e\n\u003cp\u003eBecause differentiation efficiency can vary among human pluripotent stem cell lines, users should maintain consistent starting-cell quality, confirm appropriate iPSC confluence, control aggregate diameter, and validate hepatic differentiation using laboratory-selected morphology, marker, or functional assays.\u003c\/p\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the XR Liver Organoid Differentiation Kit used for?\u003c\/summary\u003e\n\u003cp\u003eThe XR Liver Organoid Differentiation Kit is used to guide human pluripotent stem cells, including human iPSCs, through a staged differentiation workflow that produces three-dimensional liver organoid cultures for research applications.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan this kit be used to generate human iPSC liver organoids?\u003c\/summary\u003e\n\u003cp\u003eYes. The supplied protocol begins with adherent human iPSC cultures and uses sequential differentiation media followed by matrix embedding to support human iPSC-derived liver organoid formation.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow long does liver organoid differentiation take?\u003c\/summary\u003e\n\u003cp\u003eThe documented workflow begins at Day 0 and continues through Day 20 or longer. Early differentiation occurs during Days 0–5, matrix-embedded organoid formation begins during Days 6–10, and Medium F is used from Day 11 through Day 20 and beyond for continued maturation.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat starting iPSC confluence is recommended?\u003c\/summary\u003e\n\u003cp\u003eThe supplied protocol recommends beginning differentiation when iPSCs in a 6-well plate reach approximately 80% confluence.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat media are included in the liver organoid differentiation kit?\u003c\/summary\u003e\n\u003cp\u003eThe kit contains six stage-specific components: Liver Organoid Differentiation Media A, B, C, D, E, and F. The supplied volumes are 6 mL, 6 mL, 6 mL, 12 mL, 20 mL, and 50 mL, respectively.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eDoes the protocol use two-dimensional or three-dimensional culture?\u003c\/summary\u003e\n\u003cp\u003eIt uses both. Initial hepatic differentiation is performed in adherent 6-well plate culture. During Days 6–10, cell aggregates are embedded in an organoid culture matrix and transferred to a 24-well plate for three-dimensional liver organoid culture.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eIs an organoid culture matrix required?\u003c\/summary\u003e\n\u003cp\u003eYes. The supplied protocol requires differentiated cell aggregates to be combined with an organoid culture matrix before three-dimensional culture. The matrix is not listed among the supplied A–F media and should be obtained separately.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat aggregate size is recommended before matrix embedding?\u003c\/summary\u003e\n\u003cp\u003eThe protocol recommends controlling the cell aggregate diameter at approximately 100 μm before centrifugation and matrix embedding.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow much matrix-cell suspension is added to each well?\u003c\/summary\u003e\n\u003cp\u003eThe documented workflow uses 100 μL of matrix-cell suspension placed in the center of each well of a 24-well plate. The matrix is then polymerized for 30 minutes at 37°C before medium is added.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhich hepatic markers are shown in the product data?\u003c\/summary\u003e\n\u003cp\u003eThe supplied immunofluorescence images show HNF4α and albumin expression in the differentiated liver organoid cultures.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan these human liver organoids be used for drug screening?\u003c\/summary\u003e\n\u003cp\u003eThe kit is intended to generate a human liver organoid research model that may be used in drug-screening studies. Each laboratory should validate organoid quality, assay performance, and compound-response criteria for its specific experimental design.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan this kit support liver disease modeling?\u003c\/summary\u003e\n\u003cp\u003eYes. Human iPSC-derived liver organoids may be used as an in vitro platform for liver disease-modeling research, subject to the characteristics of the selected iPSC line and the laboratory’s disease-specific validation strategy.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eAre results expected to be identical across different iPSC lines?\u003c\/summary\u003e\n\u003cp\u003eNo. Differentiation efficiency, morphology, marker expression, and maturation can vary among human iPSC lines. Starting-cell quality, passage history, confluence, aggregate size, matrix handling, and culture technique should be controlled and optimized as needed.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cp\u003e\u003cstrong\u003eResearch Use Only:\u003c\/strong\u003e This product is intended for laboratory research and is not intended for diagnostic, therapeutic, or clinical use.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"XRbio","offers":[{"title":"1Kit","offer_id":52798318510261,"sku":"XR-iLiverOrg-Kit","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/2_85512595-8641-407a-aad4-9d277a3ac587.jpg?v=1784009248","url":"https:\/\/biofargo.com\/products\/xr-ipsc-derived-liver-organoid-differentiation-kit","provider":"Biofargo","version":"1.0","type":"link"}