{"product_id":"xr-ipsc-derived-imsc-differentiation-kit","title":"XR iPSC-Derived iMSC Differentiation Kit","description":"\u003c!--\nSEO TITLE:\niPSC to iMSC Differentiation Kit | XR\n\nMETA DESCRIPTION:\nDifferentiate iPSCs into expandable iMSCs with a staged A\/B\/C induction workflow designed for consistent mesenchymal stem cell research.\n\nRECOMMENDED URL:\n\/products\/ipsc-to-imsc-differentiation-kit\n\nPRIMARY KEYWORDS:\niMSC Differentiation Kit\niPSC to iMSC Differentiation\niPSC-Derived MSC\niPSC-Derived Mesenchymal Stem Cells\niPSC MSC Differentiation Medium\n\nSECONDARY KEYWORDS:\nMesenchymal Differentiation Kit\niMSC Induction Medium\niPSC Differentiation Protocol\nMesenchymal-Like Stem Cells\niMSC Culture\nEmbryoid Body Differentiation\nMesenchymal Stem Cell Research\n--\u003e\n\u003cstyle\u003e\n  \/* 现代专业风全局样式 *\/\n  .pdp-container {\n    font-family: -apple-system, BlinkMacSystemFont, \"Segoe UI\", Roboto, Helvetica, Arial, sans-serif;\n    color: #333333;\n    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.pdp-container summary:hover {\n    color: #328CC1;\n  }\n  \n  .pdp-container details p {\n    margin-top: 12px;\n    margin-bottom: 0;\n    padding-top: 12px;\n    border-top: 1px dashed #E0E0E0;\n    color: #555555;\n  }\n\n  \/* 手机端间距微调 *\/\n  @media (max-width: 768px) {\n    .pdp-container h2 {\n      font-size: 20px;\n      margin-top: 30px;\n    }\n\n    .pdp-container h3 {\n      font-size: 16px;\n    }\n\n    .pdp-container p,\n    .pdp-container li {\n      font-size: 14px;\n    }\n\n    .pdp-workflow {\n      grid-template-columns: 1fr;\n    }\n  }\n\u003c\/style\u003e\n\u003cdiv class=\"pdp-container\"\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eGenerating mesenchymal-like stem cells from induced pluripotent stem cells can be difficult to standardize across routine laboratory workflows. Variations in embryoid body formation, cell seeding, attachment, medium transitions, and passage timing may affect differentiation consistency and make it harder to obtain a uniform, expandable iMSC population.\u003c\/p\u003e\n\u003cp\u003eXR iPSC-Derived iMSC Differentiation Kit is a staged culture system designed to support the differentiation of induced pluripotent stem cells into mesenchymal-like stem cells. The workflow uses three sequential media—iMSC Induction Medium A, Medium B, and Medium C—to guide cells through embryoid body formation, attachment, mesenchymal induction, and subsequent iMSC expansion.\u003c\/p\u003e\n\u003cp\u003eThis iMSC differentiation kit provides researchers with a structured iPSC-to-iMSC differentiation protocol intended to reduce workflow variability and support the generation of consistent iPSC-derived mesenchymal stem cell cultures. The resulting cells can be passaged after reaching approximately 90% confluence and may be evaluated by flow cytometry from passage 3 onward.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cdiv class=\"pdp-table-responsive\"\u003e\n\u003ctable class=\"pdp-table\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eXR iPSC-Derived iMSC Differentiation Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eProduct Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eiPSC-to-iMSC differentiation culture kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStarting Cell Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eInduced pluripotent stem cells (iPSCs)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTarget Cell Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eiPSC-derived mesenchymal-like stem cells (iMSCs)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eCulture Format\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eEmbryoid body formation followed by adherent induction and expansion\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eInduction System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThree-stage medium workflow using Media A, B, and C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eInitial Cell Concentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e150,000 cells\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eInitial Seeding Density\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e15,000 cells per well in a low-attachment 96-well U-bottom plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eInitial Culture Volume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e200 μL per well\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eROCK Inhibitor\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e10 μM Y-27632 during initial single-cell seeding\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended Characterization\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFlow cytometry at passage 3 or later\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGrade\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFor research use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003cdiv class=\"pdp-table-responsive\"\u003e\n\u003ctable class=\"pdp-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eVolume\u003c\/th\u003e\n\u003cth\u003eWorkflow Stage\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eiMSC Induction Medium A\u003c\/td\u003e\n\u003ctd\u003e30 mL\u003c\/td\u003e\n\u003ctd\u003eEarly embryoid body induction and post-disruption attachment stage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eiMSC Induction Medium B\u003c\/td\u003e\n\u003ctd\u003e20 mL\u003c\/td\u003e\n\u003ctd\u003eIntermediate iMSC induction stage beginning on Day 8\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eiMSC Induction Medium C\u003c\/td\u003e\n\u003ctd\u003e100 mL\u003c\/td\u003e\n\u003ctd\u003eiMSC culture and expansion stage beginning on Day 11\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eDesigned for directed differentiation of iPSCs into mesenchymal-like stem cells\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThree-stage iMSC induction medium system with defined workflow transitions\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eUses embryoid body formation to initiate the iPSC-to-iMSC differentiation process\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSupports transition from suspension embryoid bodies to adherent mesenchymal-like cultures\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eProvides a structured timeline from Day 0 seeding through Day 11 iMSC culture\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSupports continued culture and passaging after cells reach approximately 90% confluence\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSuitable for downstream characterization of passage 3 or later iMSC cultures\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eDesigned to reduce variability associated with independently prepared induction media\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eiPSC to iMSC Differentiation Workflow\u003c\/h2\u003e\n\u003cp\u003eThe differentiation procedure begins with single-cell iPSC preparation and controlled embryoid body formation. Sequential medium changes then guide the developing cell aggregates through attachment, mesenchymal induction, and iMSC expansion.\u003c\/p\u003e\n\u003cdiv class=\"pdp-workflow\"\u003e\n\u003cdiv class=\"pdp-workflow-step\"\u003e\n\u003cstrong\u003eDay 0\u003c\/strong\u003e\n\u003cp\u003eSeed single-cell iPSCs in a low-attachment 96-well U-bottom plate to form embryoid bodies.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"pdp-workflow-step\"\u003e\n\u003cstrong\u003eDay 2\u003c\/strong\u003e\n\u003cp\u003eTransfer embryoid bodies to a low-attachment 6-well plate and begin culture with Medium A.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"pdp-workflow-step\"\u003e\n\u003cstrong\u003eDay 5–8\u003c\/strong\u003e\n\u003cp\u003eDisrupt embryoid bodies, transfer cells to a coated adherent plate, and continue sequential induction.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"pdp-workflow-step\"\u003e\n\u003cstrong\u003eDay 11+\u003c\/strong\u003e\n\u003cp\u003eChange to Medium C for iMSC culture and passage cells after they reach the recommended confluence.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- WORKFLOW IMAGE PLACEHOLDER --\u003e\n\u003cdiv style=\"text-align: center;\" class=\"pdp-img-container\"\u003e\n\u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/e21f0222-2823-4eb3-9ef4-a95489efee30.png?v=1783996343\" alt=\"iPSC to iMSC differentiation protocol timeline from embryoid body formation to passage 3 iMSC culture\"\u003e\n\u003cp class=\"pdp-img-caption\"\u003eRepresentative workflow from Day 0 embryoid body formation to adherent iMSC induction and expansion.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch2\u003eProtocol Overview\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eDigest the iPSC culture into single cells and prepare a cell suspension at 150,000 cells\/mL using iPSC culture medium supplemented with 10 μM Y-27632.\u003c\/li\u003e\n\u003cli\u003eAdd 15,000 cells to each well of a low-attachment 96-well U-bottom plate. Bring the final volume to 200 μL per well, centrifuge at 100 × g for 5 minutes, designate this as Day 0, and culture without disturbance for 48 hours.\u003c\/li\u003e\n\u003cli\u003eOn Day 2, transfer four to five embryoid bodies to each low-attachment 6-well plate and continue culture in iMSC Induction Medium A for 3 days.\u003c\/li\u003e\n\u003cli\u003eOn Day 5, collect the embryoid bodies in a 1.5 mL centrifuge tube. Mechanically disrupt the aggregates using a 10 μL pipette tip, resuspend them in Medium A, and seed them into a standard 6-well plate that has been prepared with an appropriate coating solution.\u003c\/li\u003e\n\u003cli\u003eOn Day 8, replace the culture medium with iMSC Induction Medium B and continue culture for 3 days.\u003c\/li\u003e\n\u003cli\u003eOn Day 11, replace the culture medium with iMSC Induction Medium C. Passage the cells when they reach approximately 90% confluence.\u003c\/li\u003e\n\u003cli\u003eAfter the cells reach passage 3 or later, evaluate the cultured cell population using an appropriate flow cytometry characterization panel.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cdiv class=\"pdp-note\"\u003e\n\u003cp\u003e\u003cstrong\u003eImportant:\u003c\/strong\u003e Cell behavior may vary among iPSC lines. Researchers should confirm cell quality before induction and optimize coating, dissociation, feeding, and passage conditions for their specific cell line and laboratory workflow.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003ch3\u003eMorphological Changes During iMSC Differentiation\u003c\/h3\u003e\n\u003cp\u003eThe product documentation presents representative images from multiple stages of the differentiation workflow. Compact embryoid bodies are visible at the initial stage, followed by aggregate attachment, outward cell migration, and the development of an adherent mesenchymal-like cell population during continued culture.\u003c\/p\u003e\n\u003ch3\u003eFlow Cytometry Characterization\u003c\/h3\u003e\n\u003cp\u003eRepresentative flow cytometry histograms are provided for passage 3 iMSC cultures. These data illustrate downstream cell characterization following the staged iPSC differentiation protocol. Individual laboratories should select and validate an appropriate marker panel for their intended research application.\u003c\/p\u003e\n\u003c!-- FLOW CYTOMETRY IMAGE PLACEHOLDER --\u003e\n\u003cdiv style=\"text-align: center;\" class=\"pdp-img-container\"\u003e\n\u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/WPS_1_36d72881-c4f0-478c-b76a-6e0c810c4bd4.png?v=1783996372\" alt=\"Flow cytometry characterization of passage 3 iPSC-derived mesenchymal-like stem cells\"\u003e\n\u003cp class=\"pdp-img-caption\"\u003eRepresentative flow cytometry characterization of passage 3 iMSC cultures.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eXR iPSC-Derived iMSC Differentiation Kit is intended for laboratories that require a structured method for generating expandable mesenchymal-like cells from an iPSC starting population. The three-stage induction workflow supports research applications in which cell-source consistency, experimental repeatability, and access to renewable iPSC-derived cells are important.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eiPSC-to-iMSC differentiation:\u003c\/strong\u003e Generate mesenchymal-like stem cells from induced pluripotent stem cell cultures using a staged embryoid body and adherent induction workflow.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eiPSC-derived mesenchymal stem cell research:\u003c\/strong\u003e Establish iMSC cultures for studies of mesenchymal differentiation, cell identity, morphology, and expansion behavior.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eStem cell fate studies:\u003c\/strong\u003e Investigate the transition from pluripotent cells through embryoid body formation toward a mesenchymal-like phenotype.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCell differentiation protocol development:\u003c\/strong\u003e Use the supplied A\/B\/C induction system as a standardized starting point for optimization across different iPSC lines.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eiMSC expansion studies:\u003c\/strong\u003e Maintain and passage induced cells after the Day 11 transition to iMSC Induction Medium C.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCell characterization workflows:\u003c\/strong\u003e Prepare passage 3 or later iMSC cultures for flow cytometry and other laboratory-defined characterization assays.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eDisease modeling research:\u003c\/strong\u003e Generate iPSC-derived mesenchymal-like cells for exploratory studies using donor-specific or disease-associated iPSC lines.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eDrug discovery and screening research:\u003c\/strong\u003e Develop iMSC-based cellular systems for research-stage compound evaluation after appropriate assay validation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eRegenerative medicine research:\u003c\/strong\u003e Study iPSC-derived mesenchymal cell generation, expansion, and biological properties in nonclinical research workflows.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCell manufacturing process development:\u003c\/strong\u003e Evaluate scalable and repeatable culture strategies for producing iPSC-derived mesenchymal-like cell populations in a research setting.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eWhy Use an iPSC-Derived iMSC Workflow?\u003c\/h2\u003e\n\u003cp\u003ePrimary mesenchymal stem cell research may be affected by limited tissue availability, donor-to-donor variability, and changes in proliferative behavior during extended culture. An iPSC-derived approach provides a renewable starting cell source and enables researchers to establish mesenchymal-like cultures from selected iPSC lines under a controlled laboratory workflow.\u003c\/p\u003e\n\u003cp\u003eXR iPSC-Derived iMSC Differentiation Kit combines embryoid body formation with sequential induction media to organize the differentiation process into clearly defined stages. This format helps laboratories standardize medium transitions and culture timing while retaining the flexibility to validate cell identity and performance according to their own experimental requirements.\u003c\/p\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the XR iPSC-Derived iMSC Differentiation Kit used for?\u003c\/summary\u003e\n\u003cp\u003eThe kit is designed for the staged differentiation of induced pluripotent stem cells into mesenchymal-like stem cells. It supports embryoid body formation, adherent induction, and subsequent iMSC culture and expansion.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is included in the iMSC differentiation kit?\u003c\/summary\u003e\n\u003cp\u003eThe kit contains 30 mL of iMSC Induction Medium A, 20 mL of iMSC Induction Medium B, and 100 mL of iMSC Induction Medium C.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow does the iPSC-to-iMSC differentiation protocol work?\u003c\/summary\u003e\n\u003cp\u003eThe workflow begins by forming embryoid bodies from single-cell iPSCs. The embryoid bodies are cultured with Medium A, mechanically disrupted and transferred to a coated adherent plate, followed by sequential culture with Medium B and Medium C.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow long does the initial iMSC induction process take?\u003c\/summary\u003e\n\u003cp\u003eThe scheduled medium transitions extend from Day 0 through Day 11. After Day 11, the cells continue to grow in Medium C and may be passaged when they reach approximately 90% confluence.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow many iPSCs are seeded at the beginning of differentiation?\u003c\/summary\u003e\n\u003cp\u003eThe supplied protocol uses a suspension concentration of 150,000 cells\/mL and an initial seeding density of 15,000 cells per well in a low-attachment 96-well U-bottom plate.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eIs Y-27632 used during the initial iPSC seeding step?\u003c\/summary\u003e\n\u003cp\u003eYes. The protocol specifies preparation of the initial single-cell suspension in iPSC culture medium supplemented with 10 μM Y-27632.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhy are embryoid bodies used in this iMSC differentiation workflow?\u003c\/summary\u003e\n\u003cp\u003eEmbryoid body formation provides an intermediate stage between the starting iPSC culture and the subsequent adherent mesenchymal induction steps. The supplied workflow begins with controlled aggregate formation before sequential exposure to the iMSC induction media.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhen should the induced iMSC cultures be passaged?\u003c\/summary\u003e\n\u003cp\u003eAfter the Day 11 change to Medium C, the cells may be passaged when the culture reaches approximately 90% confluence.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhen can iPSC-derived iMSCs be characterized by flow cytometry?\u003c\/summary\u003e\n\u003cp\u003eThe product protocol recommends flow cytometry characterization after the cells have reached passage 3 or later. Researchers should select a marker panel appropriate for their laboratory and intended application.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan this kit be used with every iPSC line without optimization?\u003c\/summary\u003e\n\u003cp\u003eDifferent iPSC lines may vary in growth, aggregate formation, attachment, and differentiation behavior. Cell-line-specific optimization and validation may therefore be required before routine use.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eIs this product intended for clinical or therapeutic use?\u003c\/summary\u003e\n\u003cp\u003eNo. XR iPSC-Derived iMSC Differentiation Kit is intended for research use only and is not intended for diagnostic, therapeutic, or direct clinical use.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cp\u003e\u003cem\u003eFor Research Use Only. Not for diagnostic or therapeutic use.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003cscript type=\"application\/ld+json\"\u003e\n{\n  \"@context\": \"https:\/\/schema.org\",\n  \"@type\": \"Product\",\n  \"name\": \"XR iPSC-Derived iMSC Differentiation Kit\",\n  \"description\": \"A staged iPSC-to-iMSC differentiation culture kit containing induction media A, B, and C for embryoid body formation, mesenchymal induction, and continued iMSC culture.\",\n  \"brand\": {\n    \"@type\": \"Brand\",\n    \"name\": \"XR\"\n  },\n  \"category\": \"Stem Cell Differentiation Reagents\",\n  \"keywords\": [\n    \"iMSC Differentiation Kit\",\n    \"iPSC to iMSC Differentiation\",\n    \"iPSC-Derived MSC\",\n    \"iPSC-Derived Mesenchymal Stem Cells\",\n    \"iPSC MSC Differentiation Medium\",\n    \"Mesenchymal Differentiation Kit\",\n    \"iMSC Induction Medium\",\n    \"Embryoid Body Differentiation\"\n  ],\n  \"additionalProperty\": [\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Starting Cell Type\",\n      \"value\": \"Induced pluripotent stem cells\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Target Cell Type\",\n      \"value\": \"iPSC-derived mesenchymal-like stem cells\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"iMSC Induction Medium A\",\n      \"value\": \"30 mL\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"iMSC Induction Medium B\",\n      \"value\": \"20 mL\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"iMSC Induction Medium C\",\n      \"value\": \"100 mL\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Recommended Characterization Stage\",\n      \"value\": \"Passage 3 or later\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Research Grade\",\n      \"value\": \"For Research Use Only\"\n    }\n  ]\n}\n\u003c\/script\u003e \u003cscript type=\"application\/ld+json\"\u003e\n{\n  \"@context\": \"https:\/\/schema.org\",\n  \"@type\": \"FAQPage\",\n  \"mainEntity\": [\n    {\n      \"@type\": \"Question\",\n      \"name\": \"What is the XR iPSC-Derived iMSC Differentiation Kit used for?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"The kit is designed for the staged differentiation of induced pluripotent stem cells into mesenchymal-like stem cells. It supports embryoid body formation, adherent induction, and subsequent iMSC culture and expansion.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"What is included in the iMSC differentiation kit?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"The kit contains 30 mL of iMSC Induction Medium A, 20 mL of iMSC Induction Medium B, and 100 mL of iMSC Induction Medium C.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"How does the iPSC-to-iMSC differentiation protocol work?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"The workflow begins by forming embryoid bodies from single-cell iPSCs. 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After Day 11, the cells continue to grow in Medium C and may be passaged when they reach approximately 90% confluence.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"How many iPSCs are seeded at the beginning of differentiation?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"The supplied protocol uses a suspension concentration of 150,000 cells per mL and an initial seeding density of 15,000 cells per well in a low-attachment 96-well U-bottom plate.\"\n      }\n    },\n    {\n      \"@type\": \"Question\",\n      \"name\": \"Is Y-27632 used during the initial iPSC seeding step?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"Yes. 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