{"product_id":"xr-intestinal-organoid-digestion-solution","title":"XR Intestinal Organoid Digestion Solution","description":"\u003c!--\nSEO TITLE:\nIntestinal Crypt Isolation Solution | Organoid Tissue Digestion\n\nMETA DESCRIPTION:\nIsolate intact intestinal crypts from adult or mouse tissue with a gentle, optimized digestion solution designed to support intestinal organoid establishment.\n\nRECOMMENDED URL:\n\/products\/intestinal-crypt-isolation-solution\n\nPRIMARY KEYWORDS:\nIntestinal Crypt Isolation Solution\nIntestinal Tissue Digestion\nIntestinal Tissue Dissociation\nIntestinal Crypt Isolation\nIntestinal Organoid Culture\n\nSECONDARY KEYWORDS:\nIntestinal Organoid Dissociation\nIntestinal Crypt Extraction\nSmall Intestinal Crypt Isolation\nColon Crypt Isolation\nMouse Intestinal Organoid\nAdult Intestinal Tissue\nGentle Tissue Dissociation\nIntestinal Stem Cell Isolation\nIntestinal Organoid Establishment\nIntestinal Organoid Culture Workflow\nHigh Crypt Integrity\nOrganoid Sample Preparation\nOrganoid Tissue Digestion Solution\n--\u003e\n\u003cstyle\u003e\n  \/* 现代专业风全局样式 *\/\n  .pdp-container {\n    font-family: -apple-system, BlinkMacSystemFont, \"Segoe UI\", Roboto, Helvetica, Arial, sans-serif;\n    color: #333333;\n    line-height: 1.6;\n    max-width: 1200px;\n    margin: 0 auto;\n    padding: 15px;\n  }\n  \n  \/* 标题样式升级 *\/\n  .pdp-container h2 {\n    font-size: 24px;\n    color: #0B3C5D;\n    border-bottom: 2px solid #328CC1;\n    padding-bottom: 8px;\n    margin-top: 40px;\n    margin-bottom: 20px;\n    font-weight: 600;\n  }\n  \n  .pdp-container h3 {\n    font-size: 18px;\n    color: #1D2731;\n    margin-top: 30px;\n    margin-bottom: 15px;\n    font-weight: 600;\n  }\n  \n  \/* 段落与列表 *\/\n  .pdp-container p {\n    margin-bottom: 16px;\n    font-size: 15px;\n    color: #4A4A4A;\n    text-align: justify;\n  }\n  \n  .pdp-container ul,\n  .pdp-container ol {\n    padding-left: 20px;\n    margin-bottom: 24px;\n  }\n  \n  .pdp-container li {\n    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.pdp-note p {\n    margin: 0;\n    color: #44515A;\n  }\n\n  \/* 精美FAQ折叠面板样式 *\/\n  .pdp-container details {\n    background-color: #F8F9FA;\n    border: 1px solid #E0E0E0;\n    border-radius: 6px;\n    margin-bottom: 12px;\n    padding: 14px 18px;\n    transition: all 0.3s ease;\n  }\n  \n  .pdp-container details[open] {\n    background-color: #FFFFFF;\n    border-color: #328CC1;\n    box-shadow: 0 4px 10px rgba(50, 140, 193, 0.1);\n  }\n  \n  .pdp-container summary {\n    font-weight: 600;\n    color: #0B3C5D;\n    cursor: pointer;\n    font-size: 15px;\n    outline: none;\n    user-select: none;\n  }\n  \n  .pdp-container summary:hover {\n    color: #328CC1;\n  }\n  \n  .pdp-container details p {\n    margin-top: 12px;\n    margin-bottom: 0;\n    padding-top: 12px;\n    border-top: 1px dashed #E0E0E0;\n    color: #555555;\n  }\n\n  \/* 手机端间距微调 *\/\n  @media (max-width: 768px) {\n    .pdp-container h2 {\n      font-size: 20px;\n      margin-top: 30px;\n    }\n\n    .pdp-container h3 {\n      font-size: 16px;\n    }\n\n    .pdp-container p,\n    .pdp-container li {\n      font-size: 14px;\n    }\n  }\n\u003c\/style\u003e\n\u003cdiv class=\"pdp-container\"\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eReliable intestinal organoid establishment begins with the recovery of intact, viable intestinal crypts. During conventional intestinal tissue digestion, insufficient dissociation can leave crypts trapped within the mucosa, while excessive enzymatic or mechanical treatment may disrupt crypt architecture and reduce the ability of intestinal stem cell-containing units to initiate new organoids.\u003c\/p\u003e\n\u003cp\u003eXR Organoid Intestinal Digestion Solution is an optimized intestinal tissue digestion solution developed for controlled dissociation of adult or mouse intestinal tissue. The formulation is designed to release structurally intact crypt units from intestinal mucosal tissue while minimizing unnecessary digestion-related stress and damage.\u003c\/p\u003e\n\u003cp\u003eThis ready-to-use intestinal crypt isolation solution supports the preparation of small intestinal and colonic crypts for downstream intestinal organoid culture. Under the documented workflow, tissue is digested at 4°C and requires only gentle pipetting after digestion, helping researchers recover crypts suitable for embedding in an appropriate extracellular matrix and culture in a compatible intestinal organoid medium.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cdiv class=\"pdp-table-responsive\"\u003e\n\u003ctable class=\"pdp-table\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eXR Organoid Intestinal Digestion Solution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eXRIOTDIGST-100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eProduct Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIntestinal tissue digestion and crypt isolation solution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePrimary Sample Types\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAdult or mouse intestinal tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTarget Structures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eSmall intestinal crypts and colonic crypts\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eEndotoxin\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e≤0.1 EU\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eFiltration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026lt;0.2 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBacteria Detection\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNot detected\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eFungi Detection\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNot detected\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e15–25°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eShelf Life\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGrade\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFor research use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eOptimized specifically for intestinal tissue digestion and intestinal crypt isolation\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eDesigned for adult or mouse intestinal tissue sample preparation\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSupports recovery of intact small intestinal and colonic crypt units\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eUses controlled low-temperature digestion to help reduce excessive tissue damage\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eRequires only gentle pipetting after the documented digestion step\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eHelps preserve crypt structure for downstream intestinal organoid establishment\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSuitable for organoid culture workflows involving crypt extraction, matrix embedding and expansion\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eLow-endotoxin formulation filtered through a \u0026lt;0.2 μm process\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eConvenient room-temperature storage at 15–25°C\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eHow It Works\u003c\/h2\u003e\n\u003cp\u003eIntestinal crypt isolation requires separation of epithelial crypt structures from surrounding mucosal tissue without unnecessarily breaking the recovered units into dispersed single cells. XR Organoid Intestinal Digestion Solution is formulated to support this balance through a controlled intestinal tissue dissociation workflow.\u003c\/p\u003e\n\u003cp\u003eAfter the intestinal sample has been cleaned and cut into small fragments, the tissue is incubated with the digestion solution at 4°C. Gentle pipetting following digestion helps release crypt structures. The suspension is then filtered and centrifuged to collect the recovered crypt-containing fraction for downstream organoid culture.\u003c\/p\u003e\n\u003cdiv class=\"pdp-note\"\u003e\n\u003cp\u003e\u003cstrong\u003eImportant:\u003c\/strong\u003e The documented 25–30 minute digestion period is a reference condition. Intestinal region, tissue thickness, sample source and fragment size may affect dissociation efficiency. Monitor crypt release and avoid excessive mechanical pipetting or prolonged digestion.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch2\u003eRecommended Workflow\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eClean the intestinal tissue and cut it into approximately 1 mm × 1 mm fragments.\u003c\/li\u003e\n\u003cli\u003eAdd 10 mL of precooled PBS and agitate the tube to wash the intestinal fragments and remove intestinal contents.\u003c\/li\u003e\n\u003cli\u003eRepeat the PBS washing process three times.\u003c\/li\u003e\n\u003cli\u003eAdd 10 mL of XR Organoid Intestinal Digestion Solution.\u003c\/li\u003e\n\u003cli\u003eDigest the tissue on a shaker at 4°C for approximately 25 minutes.\u003c\/li\u003e\n\u003cli\u003eRemove the digestion solution after incubation.\u003c\/li\u003e\n\u003cli\u003eAdd 5 mL of an appropriate termination solution to stop digestion.\u003c\/li\u003e\n\u003cli\u003eGently pipette the digested intestinal fragments using a 5 mL pipette.\u003c\/li\u003e\n\u003cli\u003eFilter the tissue suspension through a 70 μm cell strainer.\u003c\/li\u003e\n\u003cli\u003eCentrifuge at 300 × g for 5 minutes and remove the supernatant.\u003c\/li\u003e\n\u003cli\u003eProcess the recovered crypt fraction using the appropriate matrix and intestinal organoid culture medium for the intended workflow.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cdiv class=\"pdp-note\"\u003e\n\u003cp\u003e\u003cstrong\u003eWorkflow note:\u003c\/strong\u003e The termination solution, extracellular matrix and intestinal organoid culture medium are separate workflow components unless specifically stated in the ordering information.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003ch3\u003eSmall Intestinal and Colonic Crypt Recovery\u003c\/h3\u003e\n\u003cp\u003eUnder the documented test conditions, intestinal tissue was digested at 4°C for approximately 25–30 minutes. Following gentle pipetting with a 5 mL pipette, crypt structures could be recovered from both small intestinal and colonic tissue samples.\u003c\/p\u003e\n\u003c!-- IMAGE 2 POSITION: Small intestinal and colon crypt microscopy results\n     Replace PASTE_CRYPT_RESULTS_IMAGE_URL_HERE with the Shopify CDN image URL.\n\n     Recommended alt:\n     Small intestinal crypt and colon crypt isolation results after intestinal tissue digestion\n--\u003e\n\u003cdiv class=\"pdp-img-container\" style=\"text-align: center;\"\u003e\n\u003cimg alt=\"Small intestinal crypt and colon crypt isolation results after intestinal tissue digestion\" loading=\"lazy\" width=\"900\" height=\"450\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/de857cd8-3860-4939-9686-51f47fd6db75.png?v=1784010991\" style=\"float: none;\"\u003e\n\u003cp class=\"pdp-img-caption\"\u003eRepresentative small intestinal crypt and colonic crypt recovery following controlled intestinal tissue digestion.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cp\u003eThe recovered crypts showed preserved structural integrity under the evaluated conditions. The product documentation indicates that the isolated crypts can be combined with a corresponding organoid culture medium and matrix to support subsequent intestinal organoid formation.\u003c\/p\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eXR Organoid Intestinal Digestion Solution is designed for intestinal tissue processing workflows in which the recovery of structurally intact crypt units is required before three-dimensional culture. It can be incorporated into organoid research workflows involving small intestinal or colonic tissue samples.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eIntestinal crypt isolation:\u003c\/strong\u003e Controlled release and collection of crypt structures from intestinal mucosal tissue.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eSmall intestinal crypt isolation:\u003c\/strong\u003e Preparation of small intestinal crypts for matrix embedding and organoid initiation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eColon crypt isolation:\u003c\/strong\u003e Recovery of colonic crypt structures for colon organoid culture workflows.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eMouse intestinal organoid culture:\u003c\/strong\u003e Preparation of mouse intestinal tissue before crypt-based organoid establishment.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eAdult intestinal tissue processing:\u003c\/strong\u003e Dissociation of adult intestinal samples for downstream organoid research.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eIntestinal organoid establishment:\u003c\/strong\u003e Sample preparation before crypt embedding in an appropriate extracellular matrix.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eIntestinal organoid expansion:\u003c\/strong\u003e Preparation of newly isolated crypt units for subsequent growth in a compatible culture system.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eIntestinal stem cell research:\u003c\/strong\u003e Recovery of crypt units containing the epithelial stem cell compartment for research applications.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eThree-dimensional intestinal culture:\u003c\/strong\u003e Tissue preparation for 3D intestinal epithelial and organoid models.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eGastrointestinal disease modeling:\u003c\/strong\u003e Establishment of intestinal organoid cultures for research into epithelial biology and disease mechanisms.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eDrug response research:\u003c\/strong\u003e Generation of intestinal organoid models for laboratory evaluation of experimental treatment responses.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eRegenerative biology research:\u003c\/strong\u003e Study of intestinal epithelial renewal, differentiation and tissue organization using organoid platforms.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eWhy Use an Intestinal-Specific Digestion Solution?\u003c\/h2\u003e\n\u003cp\u003eGeneral-purpose tissue dissociation reagents may not provide the level of control required for intestinal crypt extraction. A digestion condition that is too weak may produce low crypt recovery, while overly aggressive treatment may fragment crypts, increase cellular stress and compromise downstream organoid formation.\u003c\/p\u003e\n\u003cp\u003eXR Organoid Intestinal Digestion Solution is optimized around the specific requirement to separate crypt structures from intestinal mucosa while maintaining their overall architecture. Its low-temperature workflow and limited mechanical handling help researchers standardize a critical early step in intestinal organoid culture.\u003c\/p\u003e\n\u003cp\u003eBy combining targeted intestinal tissue dissociation with gentle crypt recovery, the solution is suitable for laboratories seeking a more controlled approach to small intestinal and colon organoid sample preparation.\u003c\/p\u003e\n\u003ch2\u003eFactors That Can Affect Crypt Isolation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eTissue fragment size:\u003c\/strong\u003e Uneven or oversized fragments may digest inconsistently.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eIntestinal region:\u003c\/strong\u003e Small intestinal and colonic tissues may differ in dissociation behavior.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eSample condition:\u003c\/strong\u003e Collection method, storage time and tissue condition may influence crypt recovery.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eDigestion duration:\u003c\/strong\u003e Insufficient incubation may reduce release, while excessive digestion may damage crypt structures.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eMechanical force:\u003c\/strong\u003e Vigorous or repeated pipetting may disrupt intact crypts.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eTemperature control:\u003c\/strong\u003e The documented workflow uses digestion at 4°C and should not be assumed to perform identically at other temperatures.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eDownstream culture system:\u003c\/strong\u003e Organoid formation also depends on matrix selection, culture medium, seeding density and sample quality.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is XR Organoid Intestinal Digestion Solution used for?\u003c\/summary\u003e\n\u003cp\u003eXR Organoid Intestinal Digestion Solution is used for controlled intestinal tissue digestion and the isolation of crypt structures from adult or mouse intestinal tissue before intestinal organoid culture.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eIs this product an intestinal crypt isolation reagent?\u003c\/summary\u003e\n\u003cp\u003eYes. It is specifically optimized as an intestinal crypt isolation solution that helps release intact crypt units from intestinal mucosal tissue under the documented low-temperature digestion workflow.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan this solution be used for small intestinal crypt isolation?\u003c\/summary\u003e\n\u003cp\u003eYes. The product documentation includes recovery results for small intestinal crypts following digestion at 4°C and gentle pipetting.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan it be used for colon crypt isolation?\u003c\/summary\u003e\n\u003cp\u003eYes. The documented applications and performance images include the isolation of colonic crypts from intestinal tissue.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eIs the product suitable for mouse intestinal organoid culture?\u003c\/summary\u003e\n\u003cp\u003eYes. The product is described for adult or mouse intestinal tissue samples and can be used during the crypt isolation stage of a mouse intestinal organoid culture workflow.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan it be used with adult intestinal tissue?\u003c\/summary\u003e\n\u003cp\u003eYes. The product documentation describes its use with adult intestinal tissue. Digestion conditions should still be monitored and optimized according to tissue source, intestinal region and sample condition.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eDoes the solution dissociate intestinal tissue into single cells?\u003c\/summary\u003e\n\u003cp\u003eThe documented workflow is intended primarily to release intact crypt units rather than completely dissociate the sample into a single-cell suspension. The final outcome may vary with digestion time and mechanical handling.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhy is intestinal crypt integrity important for organoid culture?\u003c\/summary\u003e\n\u003cp\u003eIntact crypt units contain the epithelial compartment required to initiate intestinal organoid growth. Excessive digestion or vigorous pipetting may disrupt crypt architecture and reduce the quality of the recovered starting material.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat digestion temperature is recommended?\u003c\/summary\u003e\n\u003cp\u003eThe documented workflow uses digestion at 4°C on a shaker. The product should not be assumed to provide the same results at other temperatures without additional optimization.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow long should intestinal tissue be digested?\u003c\/summary\u003e\n\u003cp\u003eThe documented workflow uses approximately 25 minutes, while the performance notes describe a 25–30 minute range. The actual endpoint should be determined by monitoring crypt release and the condition of the tissue.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow much digestion solution is used in the documented workflow?\u003c\/summary\u003e\n\u003cp\u003eThe provided procedure uses 10 mL of intestinal digestion solution for the prepared intestinal tissue fragments. Appropriate volume may depend on tissue quantity and vessel size.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eIs mechanical dissociation required after digestion?\u003c\/summary\u003e\n\u003cp\u003eYes. The documented workflow uses gentle pipetting with a 5 mL pipette after digestion. Excessive pipetting should be avoided because it may damage crypt structures.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat cell strainer is used after intestinal tissue digestion?\u003c\/summary\u003e\n\u003cp\u003eThe documented protocol uses a 70 μm cell strainer to filter the digested tissue suspension before centrifugation.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat centrifugation condition is recommended for crypt collection?\u003c\/summary\u003e\n\u003cp\u003eThe provided workflow specifies centrifugation at 300 × g for 5 minutes, followed by removal of the supernatant.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eDoes this product include an organoid culture medium or matrix?\u003c\/summary\u003e\n\u003cp\u003eThe product is an intestinal tissue digestion solution. A compatible intestinal organoid culture medium, extracellular matrix and any required termination solution should be selected separately unless they are specifically included in the ordered configuration.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow should XR Organoid Intestinal Digestion Solution be stored?\u003c\/summary\u003e\n\u003cp\u003eThe product should be stored at 15–25°C. The stated shelf life is 12 months under the specified storage conditions.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eIs the product sterile filtered?\u003c\/summary\u003e\n\u003cp\u003eThe product specification states that it is filtered through a process with a pore size below 0.2 μm. Bacteria and fungi were not detected in the documented quality testing.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the endotoxin specification?\u003c\/summary\u003e\n\u003cp\u003eThe stated endotoxin specification is ≤0.1 EU\/mL.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eIs this intestinal tissue digestion solution intended for clinical use?\u003c\/summary\u003e\n\u003cp\u003eNo. XR Organoid Intestinal Digestion Solution is intended for research use only and is not intended for diagnostic, therapeutic or clinical applications.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cp\u003e\u003cem\u003eFor Research Use Only. Not for use in diagnostic or therapeutic procedures.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c!-- Product Schema --\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c!-- FAQ Schema --\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"XRbio","offers":[{"title":"100mL","offer_id":52798351573173,"sku":"XRIOTDIGST-100","price":0.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":52798351605941,"sku":"XRIOTDIGST-500","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/2_721ee994-009e-4611-9678-d103de8331c1.jpg?v=1784011320","url":"https:\/\/biofargo.com\/products\/xr-intestinal-organoid-digestion-solution","provider":"Biofargo","version":"1.0","type":"link"}