{"product_id":"xr-instantselect-hesc-ipsc-passaging-reagent","title":"XR InstantSelect - hESC\/iPSC Selection \u0026 Aggregate Passaging Reagent","description":"\u003c!--\nSEO TITLE:\nXR InstantSelect Enzyme-Free hESC\/iPSC Passaging Reagent\n\nMETA DESCRIPTION:\nEnzyme-free hESC\/iPSC passaging reagent for rapid selective dissociation of undifferentiated cells into 100–200 μm aggregates.\n\nRECOMMENDED URL:\n \/products\/xr-instantselect-hesc-ipsc-passaging-reagent\n--\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cstyle\u003e\n  \/* 现代专业风全局样式 *\/\n  .pdp-container {\n    font-family: -apple-system, BlinkMacSystemFont, \"Segoe UI\", Roboto, Helvetica, Arial, sans-serif;\n    color: #333333;\n    line-height: 1.6;\n    max-width: 1200px;\n    margin: 0 auto;\n    padding: 15px;\n  }\n  \n  \/* 标题样式升级 *\/\n  .pdp-container h2 {\n    font-size: 24px;\n    color: #0B3C5D;\n    border-bottom: 2px solid #328CC1;\n    padding-bottom: 8px;\n    margin-top: 40px;\n    margin-bottom: 20px;\n    font-weight: 600;\n  }\n  \n  .pdp-container h3 {\n    font-size: 18px;\n    color: #1D2731;\n    margin-top: 30px;\n    margin-bottom: 15px;\n    font-weight: 600;\n  }\n  \n  \/* 段落与列表 *\/\n  .pdp-container p {\n    margin-bottom: 16px;\n    font-size: 15px;\n    color: #4A4A4A;\n    text-align: justify;\n  }\n  \n  .pdp-container ul,\n  .pdp-container ol {\n    padding-left: 20px;\n    margin-bottom: 24px;\n  }\n  \n  .pdp-container li {\n    margin-bottom: 10px;\n    font-size: 15px;\n    color: #4A4A4A;\n  }\n\n  .pdp-container ul li {\n    list-style-type: square;\n  }\n  \n  .pdp-container li p {\n    margin-bottom: 0;\n    display: inline;\n  }\n\n  \/* 移动端表格响应式滑动容器 *\/\n  .pdp-table-responsive {\n    width: 100%;\n    overflow-x: auto;\n    -webkit-overflow-scrolling: touch;\n    margin: 25px 0;\n    border: 1px solid #E0E0E0;\n    border-radius: 6px;\n    box-shadow: 0 2px 5px rgba(0,0,0,0.05);\n  }\n  \n  .pdp-table-responsive::-webkit-scrollbar {\n    height: 6px;\n  }\n\n  .pdp-table-responsive::-webkit-scrollbar-thumb {\n    background-color: #CBCBCB;\n    border-radius: 4px;\n  }\n\n  \/* 专业表格样式 *\/\n  .pdp-table {\n    width: 100%;\n    border-collapse: collapse;\n    min-width: 600px;\n    background-color: #FFFFFF;\n    text-align: left;\n  }\n  \n  .pdp-table td,\n  .pdp-table th {\n    padding: 12px 16px;\n    border-bottom: 1px solid #E0E0E0;\n    font-size: 14px;\n    vertical-align: top;\n  }\n\n  .pdp-table th {\n    background-color: #0B3C5D;\n    color: #FFFFFF;\n    font-weight: 600;\n  }\n  \n  .pdp-table tr:last-child td {\n    border-bottom: none;\n  }\n  \n  .pdp-table tbody tr:nth-child(even) {\n    background-color: #F8F9FA;\n  }\n  \n  .pdp-table td strong {\n    color: #0B3C5D;\n    font-weight: 600;\n  }\n\n  \/* 图片自适应安全容器 *\/\n  .pdp-img-container {\n    max-width: 600px;\n    margin: 24px auto;\n    padding: 10px;\n    border: 1px solid #EEEEEE;\n    border-radius: 8px;\n    box-shadow: 0 4px 12px rgba(0, 0, 0, 0.05);\n    background: #FFFFFF;\n    box-sizing: border-box;\n    text-align: center;\n  }\n  \n  .pdp-img-container img {\n    max-width: 100%;\n    height: auto;\n    display: block;\n    margin: 0 auto;\n    border-radius: 4px;\n  }\n\n  .pdp-img-caption {\n    margin: 10px 0 0;\n    font-size: 13px;\n    color: #666666;\n    text-align: center;\n  }\n\n  \/* 操作提示模块 *\/\n  .pdp-note {\n    margin: 24px 0;\n    padding: 16px 18px;\n    background-color: #F4F8FB;\n   \n    border-radius: 4px;\n  }\n\n  .pdp-note p {\n    margin: 0;\n  }\n\n  \/* 精美FAQ折叠面板样式 *\/\n  .pdp-container details {\n    background-color: #F8F9FA;\n    border: 1px solid #E0E0E0;\n    border-radius: 6px;\n    margin-bottom: 12px;\n    padding: 14px 18px;\n    transition: all 0.3s ease;\n  }\n  \n  .pdp-container details[open] {\n    background-color: #FFFFFF;\n    border-color: #328CC1;\n    box-shadow: 0 4px 10px rgba(50, 140, 193, 0.1);\n  }\n  \n  .pdp-container summary {\n    font-weight: 600;\n    color: #0B3C5D;\n    cursor: pointer;\n    font-size: 15px;\n    outline: none;\n    user-select: none;\n  }\n  \n  .pdp-container summary:hover {\n    color: #328CC1;\n  }\n  \n  .pdp-container details p {\n    margin-top: 12px;\n    margin-bottom: 0;\n    padding-top: 12px;\n    border-top: 1px dashed #E0E0E0;\n    color: #555555;\n  }\n\n  \/* 手机端间距微调 *\/\n  @media (max-width: 768px) {\n    .pdp-container h2 {\n      font-size: 20px;\n      margin-top: 30px;\n    }\n\n    .pdp-container h3 {\n      font-size: 16px;\n    }\n\n    .pdp-container p,\n    .pdp-container li {\n      font-size: 14px;\n    }\n  }\n\u003c\/style\u003e\n\u003cdiv class=\"pdp-container\"\u003e\n\u003c!-- =========================\n  DESCRIPTION\n  ========================= --\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eRoutine passaging of human embryonic stem cells and induced pluripotent stem cells requires careful control of dissociation time and aggregate size. Excessive exposure to enzymatic dissociation reagents or overly vigorous pipetting can disrupt colonies, create aggregates that are too small, and increase cell stress. At the same time, cultures containing spontaneously differentiated areas may require additional manual selection before the undifferentiated population can be transferred.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eXR InstantSelect hES\/iPS Cell Aggregate Dissociation and Selection Reagent\u003c\/strong\u003e is an enzyme-free hESC and iPSC passaging reagent designed to combine rapid aggregate dissociation with selective detachment of undifferentiated cells. Its optimized ion-chelation approach supports non-enzymatic cell dissociation without the use of human- or animal-derived components or added protein.\u003c\/p\u003e\n\u003cp\u003eDuring the recommended protocol, colonies are exposed to InstantSelect for 1 minute, the working solution is aspirated, and the plate is incubated for an additional 3 minutes. Gentle tapping and limited pipetting then release the undifferentiated portion as cell aggregates approximately 100–200 μm in diameter, while differentiated regions can remain attached to the culture surface.\u003c\/p\u003e\n\u003cp\u003eThis integrated dissociation and selection workflow is intended to simplify routine human pluripotent stem cell passaging, reduce unnecessary mechanical manipulation, and help researchers obtain appropriately sized hESC or hiPSC aggregates for subsequent culture.\u003c\/p\u003e\n\u003c!-- =========================\n  SPECIFICATIONS\n  ========================= --\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cdiv class=\"pdp-table-responsive\"\u003e\n\u003ctable class=\"pdp-table\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eXR InstantSelect hES\/iPS Cell Aggregate Dissociation and Selection Reagent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eProduct Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eEnzyme-free human pluripotent stem cell passaging reagent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eCatalog Numbers\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eXRINSELECT-1-100 \/ XRINSELECT-1-500\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eAvailable Sizes\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e100 mL \/ 500 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplicable Cell Types\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDissociation Format\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCell aggregate or clump passaging\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended Aggregate Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e100–200 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended Exposure\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1 minute reagent contact followed by 3 minutes of residual incubation at 37°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eFormula Characteristics\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eEnzyme-free, protein-free, and without added human- or animal-derived components\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eEndotoxin\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e≤0.1 EU\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eFiltration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026lt;0.2 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBacteria Detection\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNot detected\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eFungi Detection\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNot detected\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRoom temperature\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eShelf Life\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUse\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eResearch use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c!-- =========================\n  FEATURES\n  ========================= --\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eIntegrated dissociation and selection:\u003c\/strong\u003e selectively releases undifferentiated hESC or hiPSC colonies while differentiated areas can remain attached to the culture vessel.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eEnzyme-free passaging:\u003c\/strong\u003e supports non-enzymatic human pluripotent stem cell dissociation without proteolytic digestion.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eRapid protocol:\u003c\/strong\u003e uses a 1-minute reagent exposure followed by a 3-minute residual incubation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eAggregate-based transfer:\u003c\/strong\u003e produces cell clumps with a recommended diameter of approximately 100–200 μm.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eLimited mechanical manipulation:\u003c\/strong\u003e colonies can be released by gentle tapping followed by only one or two pipetting steps.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eProtein-free formulation:\u003c\/strong\u003e contains no added protein and no added human- or animal-derived components.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eDesigned for hPSC culture:\u003c\/strong\u003e intended for routine passaging of human embryonic stem cells and human induced pluripotent stem cells.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eConvenient storage:\u003c\/strong\u003e supplied as a ready-to-use working solution that can be stored at room temperature.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- =========================\n  HOW IT WORKS\n  ========================= --\u003e\n\u003ch2\u003eHow XR InstantSelect Works\u003c\/h2\u003e\n\u003cp\u003eXR InstantSelect uses an optimized ion-chelation mechanism to weaken cell-to-cell and cell-to-surface interactions without enzymatic digestion. The short contact step initiates colony dissociation, while the residual reagent remaining on the cell layer continues the process during the subsequent 3-minute incubation.\u003c\/p\u003e\n\u003cp\u003eWhen the plate is gently tapped, undifferentiated colony regions detach as aggregates suitable for transfer. Spontaneously differentiated areas may remain attached, enabling selective collection of the undifferentiated portion during routine hESC or iPSC passaging.\u003c\/p\u003e\n\u003c!-- IMAGE PLACEHOLDER 2 --\u003e\n\u003cdiv style=\"text-align: center;\" class=\"pdp-img-container\"\u003e\n\u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_49dbad6f-9cc0-4da1-b828-045011520550.jpg?v=1783906759\" height=\"600\" width=\"600\" loading=\"lazy\" alt=\"XR InstantSelect rapid hESC and iPSC aggregate dissociation workflow showing 1 minute treatment and 3 minute incubation\"\u003e\n\u003cp class=\"pdp-img-caption\"\u003eRapid 1-minute exposure and 3-minute residual incubation workflow.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch3\u003eSelective Detachment of Undifferentiated Cells\u003c\/h3\u003e\n\u003cp\u003eUnlike a general-purpose cell dissociation reagent that releases the entire culture indiscriminately, InstantSelect is designed to support selective detachment. After treatment, the undifferentiated portion can be collected by gentle tapping, while visibly differentiated areas can remain on the plate for exclusion from the transferred population.\u003c\/p\u003e\n\u003c!-- IMAGE PLACEHOLDER 3 --\u003e\n\u003cdiv class=\"pdp-img-container\" style=\"text-align: center;\"\u003e\n\u003cimg height=\"600\" width=\"600\" loading=\"lazy\" alt=\"Selective detachment of undifferentiated hESC and iPSC colonies while differentiated cells remain attached\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_8102ea63-7636-4f72-9077-7edd63b2732c.jpg?v=1783907538\" style=\"float: none;\"\u003e\n\u003cp class=\"pdp-img-caption\"\u003eSelective collection of undifferentiated hPSC colonies during passaging.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c!-- =========================\n  PROTOCOL\n  ========================= --\u003e\n\u003ch2\u003eRecommended Passaging Protocol\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eRemove the spent culture medium from the hESC or hiPSC culture.\u003c\/li\u003e\n\u003cli\u003eWash the culture with calcium- and magnesium-free DPBS to remove residual medium and cellular debris.\u003c\/li\u003e\n\u003cli\u003eAdd 1 mL of XR InstantSelect to each well of a 6-well plate.\u003c\/li\u003e\n\u003cli\u003eIncubate the plate at 37°C for 1 minute.\u003c\/li\u003e\n\u003cli\u003eAspirate the InstantSelect working solution immediately after the 1-minute exposure.\u003c\/li\u003e\n\u003cli\u003eReturn the plate to the 37°C incubator for an additional 3 minutes. Residual solution on the cell surface completes the aggregate dissociation step.\u003c\/li\u003e\n\u003cli\u003eAdd 1 mL of culture medium to the treated well.\u003c\/li\u003e\n\u003cli\u003eGently tap the edge of the plate to release the undifferentiated cell layer as small aggregates.\u003c\/li\u003e\n\u003cli\u003ePipette gently one or two times to obtain aggregates approximately 100–200 μm in diameter.\u003c\/li\u003e\n\u003cli\u003eTransfer the resulting cell aggregates according to the laboratory’s established hESC or hiPSC culture procedure.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cdiv class=\"pdp-note\"\u003e\n\u003cp\u003e\u003cstrong\u003eImportant:\u003c\/strong\u003e Avoid excessive pipetting, which may produce aggregates smaller than the recommended 100–200 μm range. Actual timing may require optimization according to cell line, colony density, culture matrix, and laboratory conditions.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c!-- =========================\n  WORKFLOW COMPARISON\n  ========================= --\u003e\n\u003ch2\u003ePassaging Workflow Comparison\u003c\/h2\u003e\n\u003cdiv class=\"pdp-table-responsive\"\u003e\n\u003ctable class=\"pdp-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eWorkflow Consideration\u003c\/th\u003e\n\u003cth\u003eXR InstantSelect\u003c\/th\u003e\n\u003cth\u003eConventional Enzymatic Dissociation\u003c\/th\u003e\n\u003cth\u003eConventional EDTA-Based Passaging\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDissociation Principle\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eOptimized ion-chelation approach\u003c\/td\u003e\n\u003ctd\u003eProteolytic enzyme activity\u003c\/td\u003e\n\u003ctd\u003eChelation-based dissociation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eEnzyme-Free\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eYes\u003c\/td\u003e\n\u003ctd\u003eNo\u003c\/td\u003e\n\u003ctd\u003eYes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTransfer Format\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCell aggregates\u003c\/td\u003e\n\u003ctd\u003eOften single cells or small clusters, depending on protocol\u003c\/td\u003e\n\u003ctd\u003eCell aggregates\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended Processing Time\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1-minute contact plus 3-minute incubation\u003c\/td\u003e\n\u003ctd\u003eProtocol-dependent\u003c\/td\u003e\n\u003ctd\u003eCommonly longer than the InstantSelect workflow\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSelective Detachment\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eDesigned to release undifferentiated regions while differentiated areas remain attached\u003c\/td\u003e\n\u003ctd\u003eGenerally dissociates treated cells without selective colony enrichment\u003c\/td\u003e\n\u003ctd\u003eNot specifically designed for selective undifferentiated-cell detachment\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMechanical Handling\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eGentle tapping and one or two pipetting steps\u003c\/td\u003e\n\u003ctd\u003eProtocol-dependent\u003c\/td\u003e\n\u003ctd\u003eGentle tapping or pipetting\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c!-- =========================\n  APPLICATIONS\n  ========================= --\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eXR InstantSelect is designed for human pluripotent stem cell laboratories that require a rapid, enzyme-free method for routine aggregate passaging. Its selective dissociation workflow is particularly relevant when researchers need to transfer undifferentiated colonies while minimizing the carryover of visibly differentiated areas.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eRoutine hiPSC passaging:\u003c\/strong\u003e non-enzymatic dissociation of human induced pluripotent stem cell colonies into transferable aggregates.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eRoutine hESC passaging:\u003c\/strong\u003e aggregate-based transfer of human embryonic stem cells without proteolytic enzymes.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eHuman pluripotent stem cell maintenance:\u003c\/strong\u003e regular hPSC culture workflows requiring controlled colony breakup rather than complete single-cell dissociation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eSelective undifferentiated-cell collection:\u003c\/strong\u003e separation of detachable undifferentiated colony regions from differentiated areas that remain attached.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eClump passaging:\u003c\/strong\u003e preparation of hESC or hiPSC aggregates approximately 100–200 μm in diameter.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eColony passaging:\u003c\/strong\u003e transfer of pluripotent stem cell colonies using gentle tapping and limited pipetting.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eEnzyme-free stem cell culture workflows:\u003c\/strong\u003e replacement of proteolytic dissociation steps where aggregate-based passaging is preferred.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eReduction of differentiated-cell carryover:\u003c\/strong\u003e routine culture workflows in which visibly differentiated regions need to be excluded from the transferred cell population.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eExpansion before downstream research:\u003c\/strong\u003e passaging of hESCs or hiPSCs before laboratory-defined characterization, differentiation, gene-editing, disease-modeling, or other research workflows.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eStandardized PSC handling:\u003c\/strong\u003e laboratories seeking a short, defined contact-and-incubation sequence for routine human pluripotent stem cell passaging.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- =========================\n  CHOOSING A DISSOCIATION FORMAT\n  ========================= --\u003e\n\u003ch2\u003eWhen to Use Aggregate-Based hPSC Passaging\u003c\/h2\u003e\n\u003cp\u003eHuman pluripotent stem cells may be transferred either as aggregates or as single cells, depending on the established culture system and downstream objective. XR InstantSelect is specifically intended for aggregate-based passaging rather than complete single-cell dissociation.\u003c\/p\u003e\n\u003cp\u003eThis format is suitable when the laboratory protocol calls for maintaining cell-to-cell contact within small hESC or hiPSC clumps. The recommended 100–200 μm aggregate range provides a practical visual target during passaging and helps prevent unnecessary over-dissociation.\u003c\/p\u003e\n\u003cp\u003eFor workflows that specifically require a uniform single-cell suspension, researchers should use a dissociation method validated for that purpose instead of extending the InstantSelect incubation or applying excessive mechanical pipetting.\u003c\/p\u003e\n\u003c!-- =========================\n  FAQ\n  ========================= --\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is XR InstantSelect used for?\u003c\/summary\u003e\n\u003cp\u003eXR InstantSelect is an enzyme-free hESC and iPSC passaging reagent used to dissociate human pluripotent stem cell colonies into small aggregates. It is designed to selectively release undifferentiated colony regions while differentiated areas can remain attached to the culture vessel.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eIs XR InstantSelect an enzymatic cell dissociation reagent?\u003c\/summary\u003e\n\u003cp\u003eNo. XR InstantSelect is a non-enzymatic cell dissociation reagent. It uses an optimized ion-chelation approach instead of proteolytic enzymes to support hESC and hiPSC aggregate passaging.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhich cell types can be passaged with XR InstantSelect?\u003c\/summary\u003e\n\u003cp\u003eThe reagent is intended for human embryonic stem cells and human induced pluripotent stem cells. It is specifically designed for hESC and hiPSC colony or aggregate passaging workflows.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eDoes XR InstantSelect produce single cells or cell aggregates?\u003c\/summary\u003e\n\u003cp\u003eXR InstantSelect is designed for aggregate-based passaging. Under the recommended procedure, gentle tapping and one or two pipetting steps produce hESC or hiPSC aggregates approximately 100–200 μm in diameter.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the recommended InstantSelect treatment time?\u003c\/summary\u003e\n\u003cp\u003eAdd the reagent and incubate the culture at 37°C for 1 minute. Aspirate the solution immediately, then return the plate to 37°C for an additional 3 minutes so that residual reagent on the cell layer can complete the dissociation process.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow much InstantSelect should be used in a 6-well plate?\u003c\/summary\u003e\n\u003cp\u003eThe recommended procedure uses 1 mL of InstantSelect per well of a 6-well plate after washing the culture with calcium- and magnesium-free DPBS.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhy is the InstantSelect solution aspirated after only 1 minute?\u003c\/summary\u003e\n\u003cp\u003eThe product protocol uses a short initial contact period followed by residual incubation. After aspiration, the small amount of reagent remaining on the cell surface continues the dissociation process during the subsequent 3-minute incubation.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow does XR InstantSelect support selective dissociation of undifferentiated cells?\u003c\/summary\u003e\n\u003cp\u003eFollowing treatment, undifferentiated colony regions can be released by gently tapping the plate. Differentiated regions may remain attached, allowing the detachable undifferentiated aggregates to be collected for passaging.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat aggregate size should be targeted during hESC or iPSC passaging?\u003c\/summary\u003e\n\u003cp\u003eThe recommended aggregate diameter is approximately 100–200 μm. Pipette gently only one or two times after the colonies detach to avoid producing excessively small cell clumps.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eShould the cells be washed before adding InstantSelect?\u003c\/summary\u003e\n\u003cp\u003eYes. Wash the culture with calcium- and magnesium-free DPBS before treatment to remove residual culture medium and cellular debris.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eDoes XR InstantSelect contain protein or animal-derived components?\u003c\/summary\u003e\n\u003cp\u003eThe product is described as protein-free and does not contain added human- or animal-derived components.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan XR InstantSelect be used when a single-cell suspension is required?\u003c\/summary\u003e\n\u003cp\u003eXR InstantSelect is designed for aggregate-based passaging rather than complete single-cell dissociation. For workflows requiring a uniform single-cell suspension, use a dissociation method validated for that specific purpose.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow should XR InstantSelect be stored?\u003c\/summary\u003e\n\u003cp\u003eStore XR InstantSelect at room temperature. The stated shelf life is 12 months when stored according to the product instructions.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat pack sizes are available?\u003c\/summary\u003e\n\u003cp\u003eXR InstantSelect is available in 100 mL and 500 mL pack sizes under catalog numbers XRINSELECT-1-100 and XRINSELECT-1-500.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eIs XR InstantSelect intended for clinical use?\u003c\/summary\u003e\n\u003cp\u003eNo. XR InstantSelect is supplied for research use only and is not intended for diagnostic, therapeutic, or clinical use.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003c!-- =========================\n  IMPORTANT NOTICE\n  ========================= --\u003e\n\u003ch2\u003eImportant Notice\u003c\/h2\u003e\n\u003cp\u003eThis product is supplied for research use only. Culture response and optimal handling may vary with cell line, passage history, colony density, culture matrix, medium, and laboratory conditions. Researchers should validate the passaging procedure within their established human pluripotent stem cell culture system.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c!-- =========================\nPRODUCT SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c!-- =========================\nFAQ SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"XRbio","offers":[{"title":"100mL","offer_id":52795116650677,"sku":"XRINSELECT-1-100","price":0.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":52795116683445,"sku":"XRINSELECT-1-500","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_bb2b9a22-bc37-4a4f-b610-430e875b2be6.jpg?v=1783907070","url":"https:\/\/biofargo.com\/products\/xr-instantselect-hesc-ipsc-passaging-reagent","provider":"Biofargo","version":"1.0","type":"link"}