{"product_id":"xr-human-epithelial-cell-medium-kit","title":"XR Human Epithelial Cell Medium Kit","description":"\u003c!--\nSEO TITLE:\nPrimary Epithelial Cell Growth Medium | XR Low-Serum Culture Kit\n\nMETA DESCRIPTION:\nXR Epithelial Cell Growth Medium helps laboratories improve primary epithelial cell attachment, expansion and phenotype maintenance with a low-serum, tissue-adapted culture system.\n\nRECOMMENDED URL:\n\/products\/primary-epithelial-cell-growth-medium\n\nPRIMARY KEYWORDS:\nPrimary Epithelial Cell Growth Medium\nPrimary Epithelial Cell Culture Medium\nHuman Epithelial Cell Growth Medium\nEpithelial Cell Culture Medium\nLow-Serum Epithelial Cell Medium\n\nSECONDARY KEYWORDS:\nPrimary Human Epithelial Cell Medium\nEpithelial Cell Growth Medium Kit\nPrimary Epithelial Cell Expansion\nHuman Epithelial Cell Culture\nAirway Epithelial Cell Culture Medium\nBronchial Epithelial Cell Medium\nAlveolar Epithelial Cell Medium\nCorneal Epithelial Cell Culture Medium\nConjunctival Epithelial Cell Medium\nRetinal Pigment Epithelial Cell Medium\nRPE Cell Culture Medium\nRenal Epithelial Cell Culture Medium\nRenal Proximal Tubule Epithelial Cell Medium\nMammary Epithelial Cell Culture Medium\nProstate Epithelial Cell Medium\nSebaceous Gland Cell Culture Medium\nEGF-Supplemented Epithelial Medium\nTransferrin-Supplemented Cell Culture Medium\n--\u003e\n\n\u003cp\u003e\n\u003cscript type=\"application\/ld+json\"\u003e\n{\n  \"@context\":\"https:\/\/schema.org\",\n  \"@type\":\"Product\",\n  \"name\":\"XR Epithelial Cell Growth Medium Kit\",\n  \"description\":\"Low-serum primary epithelial cell culture medium kit designed to support cell attachment, spreading, survival, expansion and epithelial phenotype maintenance across multiple tissue-derived epithelial cell types.\",\n  \"brand\":{\n    \"@type\":\"Brand\",\n    \"name\":\"XR\"\n  },\n  \"category\":\"Primary Epithelial Cell Culture Medium\",\n  \"sku\":\"XREpiGM-500-A\",\n  \"additionalProperty\":[\n    {\n      \"@type\":\"PropertyValue\",\n      \"name\":\"Product Type\",\n      \"value\":\"Low-serum primary epithelial cell growth medium kit\"\n    },\n    {\n      \"@type\":\"PropertyValue\",\n      \"name\":\"Serum Concentration\",\n      \"value\":\"2% v\/v fetal bovine serum\"\n    },\n    {\n      \"@type\":\"PropertyValue\",\n      \"name\":\"Growth Components\",\n      \"value\":\"Recombinant human epidermal growth factor and transferrin\"\n    },\n    {\n      \"@type\":\"PropertyValue\",\n      \"name\":\"Endotoxin\",\n      \"value\":\"≤0.1 EU\/mL\"\n    },\n    {\n      \"@type\":\"PropertyValue\",\n      \"name\":\"Filtration\",\n      \"value\":\"\u003c0.2 μm\"\n    },\n    {\n      \"@type\":\"PropertyValue\",\n      \"name\":\"Antibiotics\",\n      \"value\":\"Not included\"\n    },\n    {\n      \"@type\":\"PropertyValue\",\n      \"name\":\"Antifungal Agents\",\n      \"value\":\"Not included\"\n    },\n    {\n      \"@type\":\"PropertyValue\",\n      \"name\":\"Storage\",\n      \"value\":\"Basal medium at 2–8°C; supplements at -20°C\"\n    },\n    {\n      \"@type\":\"PropertyValue\",\n      \"name\":\"Shelf Life\",\n      \"value\":\"12 months\"\n    },\n    {\n      \"@type\":\"PropertyValue\",\n      \"name\":\"Application\",\n      \"value\":\"Primary epithelial cell culture and expansion research\"\n    }\n  ]\n}\n\u003c\/script\u003e\n\n\u003cscript type=\"application\/ld+json\"\u003e\n{\n  \"@context\":\"https:\/\/schema.org\",\n  \"@type\":\"FAQPage\",\n  \"mainEntity\":[\n    {\n      \"@type\":\"Question\",\n      \"name\":\"What is primary epithelial cell growth medium?\",\n      \"acceptedAnswer\":{\n        \"@type\":\"Answer\",\n        \"text\":\"Primary epithelial cell growth medium is a specialized culture formulation designed to support the attachment, survival, spreading and proliferation of epithelial cells isolated from tissues while helping maintain epithelial morphology and phenotype.\"\n      }\n    },\n    {\n      \"@type\":\"Question\",\n      \"name\":\"What cells can be cultured with XR Epithelial Cell Growth Medium Kit?\",\n      \"acceptedAnswer\":{\n        \"@type\":\"Answer\",\n        \"text\":\"XR Epithelial Cell Growth Medium Kit can be configured for retinal pigment epithelial cells and for other epithelial cell types including bronchial, alveolar, mammary, corneal, conjunctival, renal, renal proximal tubule, sebaceous gland and prostate epithelial cells.\"\n      }\n    },\n    {\n      \"@type\":\"Question\",\n      \"name\":\"Is XR Epithelial Cell Growth Medium serum-free?\",\n      \"acceptedAnswer\":{\n        \"@type\":\"Answer\",\n        \"text\":\"No. XR Epithelial Cell Growth Medium is a low-serum formulation containing 2% v\/v fetal bovine serum. It should not be described as a serum-free epithelial cell culture medium.\"\n      }\n    },\n    {\n      \"@type\":\"Question\",\n      \"name\":\"Does the medium contain antibiotics?\",\n      \"acceptedAnswer\":{\n        \"@type\":\"Answer\",\n        \"text\":\"No. The supplied medium does not contain antibiotics or antifungal agents. An antibiotic solution may be added at 0.5–1% when required by the laboratory protocol.\"\n      }\n    },\n    {\n      \"@type\":\"Question\",\n      \"name\":\"How should the complete epithelial cell medium be prepared?\",\n      \"acceptedAnswer\":{\n        \"@type\":\"Answer\",\n        \"text\":\"Thaw the appropriate EpiGM supplement at 4°C overnight or at room temperature for 1–2 hours, then mix it completely with the Epi basal medium. For partial preparation, combine 4 mL of supplement with 96 mL of basal medium.\"\n      }\n    },\n    {\n      \"@type\":\"Question\",\n      \"name\":\"How long can the prepared complete medium be stored?\",\n      \"acceptedAnswer\":{\n        \"@type\":\"Answer\",\n        \"text\":\"The prepared complete epithelial cell growth medium can be stored at 2–8°C for up to four weeks.\"\n      }\n    }\n  ]\n}\n\u003c\/script\u003e\n\u003c\/p\u003e\n\n\u003cstyle\u003e\n\/* XR系列产品页统一专业样式 *\/\n.pdp-container {\n  font-family:-apple-system,BlinkMacSystemFont,\"Segoe UI\",Roboto,Helvetica,Arial,sans-serif;\n  color:#333333;\n  line-height:1.6;\n  max-width:1200px;\n  margin:0 auto;\n  padding:15px;\n}\n\n.pdp-container h2 {\n  font-size:24px;\n  color:#0B3C5D;\n  border-bottom:2px solid #328CC1;\n  padding-bottom:8px;\n  margin-top:40px;\n  margin-bottom:20px;\n  font-weight:600;\n}\n\n.pdp-container h3 {\n  font-size:18px;\n  color:#1D2731;\n  margin-top:30px;\n  margin-bottom:15px;\n  font-weight:600;\n}\n\n.pdp-container p {\n  margin-bottom:16px;\n  font-size:15px;\n  color:#4A4A4A;\n  text-align:justify;\n}\n\n.pdp-container ul {\n  padding-left:20px;\n  margin-bottom:24px;\n}\n\n.pdp-container li {\n  margin-bottom:10px;\n  font-size:15px;\n  color:#4A4A4A;\n  list-style-type:square;\n}\n\n.pdp-table-responsive {\n  width:100%;\n  overflow-x:auto;\n  -webkit-overflow-scrolling:touch;\n  margin:25px 0;\n  border:1px solid #E0E0E0;\n  border-radius:6px;\n}\n\n.pdp-table {\n  width:100%;\n  border-collapse:collapse;\n  min-width:650px;\n  background:#FFFFFF;\n  text-align:left;\n}\n\n.pdp-table th {\n  padding:12px 16px;\n  background:#0B3C5D;\n  color:#FFFFFF;\n  border-bottom:1px solid #E0E0E0;\n  font-size:14px;\n  font-weight:600;\n}\n\n.pdp-table td {\n  padding:12px 16px;\n  border-bottom:1px solid #E0E0E0;\n  font-size:14px;\n  vertical-align:top;\n}\n\n.pdp-table tr:nth-child(even) {\n  background:#F8F9FA;\n}\n\n.pdp-table td strong {\n  color:#0B3C5D;\n}\n\n.pdp-img-container {\n  max-width:600px;\n  margin:24px auto;\n  padding:10px;\n  border:1px solid #EEEEEE;\n  border-radius:8px;\n  background:#FFFFFF;\n  text-align:center;\n}\n\n.pdp-img-container img {\n  max-width:100%;\n  height:auto;\n  display:block;\n  margin:auto;\n}\n\n.pdp-img-caption {\n  margin:10px 0 0;\n  font-size:13px !important;\n  color:#666666 !important;\n  text-align:center !important;\n}\n\n.pdp-note {\n  margin:22px 0;\n  padding:16px 18px;\n  background:#F5F9FC;\n \n  border-radius:4px;\n}\n\n.pdp-note p {\n  margin:0;\n}\n\n.pdp-container details {\n  background:#F8F9FA;\n  border:1px solid #E0E0E0;\n  border-radius:6px;\n  margin-bottom:12px;\n  padding:14px 18px;\n}\n\n.pdp-container summary {\n  font-weight:600;\n  color:#0B3C5D;\n  cursor:pointer;\n  font-size:15px;\n}\n\n.pdp-container details p {\n  margin-top:12px;\n  margin-bottom:4px;\n}\n\n@media (max-width:768px) {\n  .pdp-container {\n    padding:10px;\n  }\n\n  .pdp-container h2 {\n    font-size:21px;\n  }\n\n  .pdp-container h3 {\n    font-size:17px;\n  }\n}\n\u003c\/style\u003e\n\n\u003cdiv class=\"pdp-container\"\u003e\n\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\n\u003cp\u003ePrimary epithelial cells can be difficult to establish and expand because limited attachment, slow spreading, early senescence and loss of epithelial characteristics may reduce experimental consistency. A general-purpose basal medium may not provide the tissue-adapted nutrients and growth support required by sensitive primary epithelial cell cultures.\u003c\/p\u003e\n\n\u003cp\u003eXR Epithelial Cell Growth Medium Kit is a low-serum primary epithelial cell culture medium developed for epithelial cells derived from respiratory, ocular, renal, mammary, prostate and other tissues. Its optimized basal formulation supports cell attachment and spreading, while the selected epithelial growth supplement provides balanced nutritional support for cell survival and proliferation.\u003c\/p\u003e\n\n\u003cp\u003eThe complete culture system contains recombinant human epidermal growth factor and transferrin together with 2% v\/v fetal bovine serum. This low-serum formulation is designed to reduce excessive serum exposure while providing the growth support needed to help delay primary epithelial cell senescence and maintain epithelial phenotype and key cellular functions.\u003c\/p\u003e\n\n\u003cp\u003eTwo tissue-oriented supplement options are available. EpiGM-B is intended for retinal pigment epithelial cell culture, while EpiGM-M supports a broader range of primary epithelial cell types, including bronchial, alveolar, mammary, corneal, conjunctival, renal, renal proximal tubule, sebaceous gland and prostate epithelial cells.\u003c\/p\u003e\n\n\u003cdiv class=\"pdp-note\"\u003e\n\u003cp\u003e\u003cstrong\u003eImportant:\u003c\/strong\u003e XR Epithelial Cell Growth Medium is a low-serum medium containing 2% v\/v fetal bovine serum. It is not a serum-free epithelial cell culture medium.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\n\u003cdiv class=\"pdp-table-responsive\"\u003e\n\u003ctable class=\"pdp-table\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eXR Epithelial Cell Growth Medium Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eProduct Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eLow-serum primary epithelial cell growth medium kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eKit Reference\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eXREpiGM-500-A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBasal Medium\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eEpi Basal Medium, 480 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSupplement Options\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eEpiGM-M Supplement, 20 mL\u003cbr\u003eEpiGM-B Supplement, 20 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSupplement References\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eXREpiGM-500-(M)B\u003cbr\u003eXREpiGM-500-(B)B\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSerum Content\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2% v\/v fetal bovine serum in the complete formulation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGrowth Components\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRecombinant human epidermal growth factor and transferrin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003epH\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e7.2–7.4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eEndotoxin\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e≤0.1 EU\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eFiltration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026lt;0.2 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBacteria Detection\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNot detected\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eFungi Detection\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNot detected\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eAntibiotics\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNot included\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eAntifungal Agents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNot included\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBasal Medium Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2–8°C, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSupplement Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e-20°C, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePrepared Medium Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2–8°C for up to 4 weeks\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eShelf Life\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eResearch Grade\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFor Research Use Only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\n\u003ch2\u003eMedium Selection Guide\u003c\/h2\u003e\n\n\u003cp\u003eSelect the supplement according to the epithelial cell type being cultured. The basal medium is combined with either EpiGM-B or EpiGM-M to create the appropriate complete primary epithelial cell growth medium.\u003c\/p\u003e\n\n\u003cdiv class=\"pdp-table-responsive\"\u003e\n\u003ctable class=\"pdp-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eComplete Medium Configuration\u003c\/th\u003e\n\u003cth\u003eRecommended Cell Types\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eEpi Basal Medium + EpiGM-B\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRetinal pigment epithelial cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eEpi Basal Medium + EpiGM-M\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eBronchial epithelial cells, alveolar epithelial cells, mammary epithelial cells, corneal epithelial cells, conjunctival epithelial cells, renal epithelial cells, renal proximal tubule epithelial cells, sebaceous gland cells and prostate epithelial cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\n\u003cul\u003e\n\u003cli\u003eLow-serum epithelial cell culture medium containing 2% v\/v fetal bovine serum\u003c\/li\u003e\n\u003cli\u003eDesigned for primary epithelial cell attachment, spreading, survival and expansion\u003c\/li\u003e\n\u003cli\u003eContains selected epithelial cell nutrients for balanced culture support\u003c\/li\u003e\n\u003cli\u003eContains recombinant human epidermal growth factor to support epithelial cell proliferation\u003c\/li\u003e\n\u003cli\u003eContains transferrin to support iron transport and cellular metabolism\u003c\/li\u003e\n\u003cli\u003eHelps reduce premature senescence during primary epithelial cell culture\u003c\/li\u003e\n\u003cli\u003eDesigned to maintain epithelial cell phenotype and key cellular functions\u003c\/li\u003e\n\u003cli\u003eTwo supplement options for retinal pigment epithelial cells and other tissue-derived epithelial cells\u003c\/li\u003e\n\u003cli\u003eLow endotoxin specification of ≤0.1 EU\/mL\u003c\/li\u003e\n\u003cli\u003eFiltered through a \u0026lt;0.2 μm filtration process\u003c\/li\u003e\n\u003cli\u003eSupplied without antibiotics or antifungal agents for flexible experimental control\u003c\/li\u003e\n\u003cli\u003eConvenient basal medium and supplement format for complete or partial preparation\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eCulture Medium Preparation\u003c\/h2\u003e\n\n\u003ch3\u003eComplete 500 mL Preparation\u003c\/h3\u003e\n\n\u003cp\u003eThaw the appropriate EpiGM supplement at 4°C overnight or at room temperature for 1–2 hours. Add the supplement to the Epi basal medium and mix thoroughly to prepare the complete epithelial cell culture medium.\u003c\/p\u003e\n\n\u003ch3\u003ePartial Preparation\u003c\/h3\u003e\n\n\u003cp\u003eFor experiments that do not require the entire kit at one time, aliquot the supplement and prepare the medium using the following ratio:\u003c\/p\u003e\n\n\u003cdiv class=\"pdp-table-responsive\"\u003e\n\u003ctable class=\"pdp-table\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eEpiGM Supplement\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eEpi Basal Medium\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e96 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eFinal Volume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\n\u003cp\u003eStore the prepared complete medium at 2–8°C and use within four weeks.\u003c\/p\u003e\n\n\u003ch3\u003eOptional Antibiotic Addition\u003c\/h3\u003e\n\n\u003cp\u003eThe product does not contain antibiotics or antifungal agents. When required by the experimental protocol, an antibiotic solution may be added at 0.5–1% of the final culture volume.\u003c\/p\u003e\n\n\u003ch2\u003eRecommended Culture Conditions\u003c\/h2\u003e\n\n\u003cdiv class=\"pdp-table-responsive\"\u003e\n\u003ctable class=\"pdp-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eCulture Stage\u003c\/th\u003e\n\u003cth\u003eRecommended Seeding Density\u003c\/th\u003e\n\u003cth\u003eMedium Change\u003c\/th\u003e\n\u003cth\u003eRecommended Passage Point\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRapid Cell Growth\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e10,000–15,000 cells\/cm²\u003c\/td\u003e\n\u003ctd\u003eChange medium every other day\u003c\/td\u003e\n\u003ctd\u003e70–80% confluence\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRoutine Passage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e5,000–8,000 cells\/cm²\u003c\/td\u003e\n\u003ctd\u003eChange medium every other day\u003c\/td\u003e\n\u003ctd\u003e70–80% confluence\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\n\u003cp\u003eThe optimal seeding density may vary according to cell source, donor, passage number, culture vessel, coating system and experimental objective. Researchers should confirm the appropriate conditions for their specific primary epithelial cell model.\u003c\/p\u003e\n\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\n\u003cp\u003eXR Epithelial Cell Growth Medium Kit supports research workflows that require controlled culture and expansion of primary epithelial cells from multiple tissue sources. Its low-serum formulation and tissue-oriented supplement options make it suitable for epithelial cell isolation, routine propagation, morphology monitoring and downstream functional studies.\u003c\/p\u003e\n\n\u003ch3\u003ePrimary Epithelial Cell Culture and Expansion\u003c\/h3\u003e\n\n\u003cul\u003e\n\u003cli\u003eEstablishment of primary epithelial cell cultures from tissue-derived samples\u003c\/li\u003e\n\u003cli\u003ePrimary human epithelial cell attachment and spreading studies\u003c\/li\u003e\n\u003cli\u003eRoutine propagation and expansion of epithelial cell populations\u003c\/li\u003e\n\u003cli\u003eEvaluation of epithelial cell morphology during in vitro culture\u003c\/li\u003e\n\u003cli\u003ePreparation of epithelial cells for downstream molecular and cellular assays\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch3\u003eAirway and Pulmonary Epithelial Cell Culture\u003c\/h3\u003e\n\n\u003cul\u003e\n\u003cli\u003eBronchial epithelial cell culture and expansion\u003c\/li\u003e\n\u003cli\u003eAirway epithelial cell growth studies\u003c\/li\u003e\n\u003cli\u003eAlveolar epithelial cell culture research\u003c\/li\u003e\n\u003cli\u003eRespiratory epithelial cell morphology and proliferation analysis\u003c\/li\u003e\n\u003cli\u003eIn vitro studies involving respiratory tissue-derived epithelial cells\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch3\u003eOcular Epithelial Cell Research\u003c\/h3\u003e\n\n\u003cul\u003e\n\u003cli\u003eRetinal pigment epithelial cell culture using EpiGM-B supplement\u003c\/li\u003e\n\u003cli\u003eRPE cell attachment, maintenance and expansion studies\u003c\/li\u003e\n\u003cli\u003eCorneal epithelial cell culture using EpiGM-M supplement\u003c\/li\u003e\n\u003cli\u003eConjunctival epithelial cell growth and morphology studies\u003c\/li\u003e\n\u003cli\u003eResearch involving ocular surface and retinal epithelial cell models\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch3\u003eRenal Epithelial Cell Culture\u003c\/h3\u003e\n\n\u003cul\u003e\n\u003cli\u003eRenal epithelial cell culture and proliferation research\u003c\/li\u003e\n\u003cli\u003eKidney epithelial cell maintenance in vitro\u003c\/li\u003e\n\u003cli\u003eRenal proximal tubule epithelial cell culture\u003c\/li\u003e\n\u003cli\u003eEvaluation of renal epithelial cell morphology and growth behavior\u003c\/li\u003e\n\u003cli\u003ePreparation of kidney-derived epithelial cells for downstream assays\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch3\u003eMammary and Prostate Epithelial Cell Research\u003c\/h3\u003e\n\n\u003cul\u003e\n\u003cli\u003ePrimary mammary epithelial cell culture and expansion\u003c\/li\u003e\n\u003cli\u003eMammary epithelial cell proliferation studies\u003c\/li\u003e\n\u003cli\u003ePrimary prostate epithelial cell culture\u003c\/li\u003e\n\u003cli\u003eProstate epithelial cell morphology and phenotype research\u003c\/li\u003e\n\u003cli\u003eIn vitro studies involving glandular epithelial cell populations\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch3\u003eSebaceous Gland Cell Culture\u003c\/h3\u003e\n\n\u003cul\u003e\n\u003cli\u003eCulture and expansion of sebaceous gland-derived cells\u003c\/li\u003e\n\u003cli\u003eEvaluation of sebaceous cell morphology and proliferation\u003c\/li\u003e\n\u003cli\u003ePreparation of sebaceous gland cells for cellular research workflows\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch3\u003eEpithelial Cell Phenotype and Function Studies\u003c\/h3\u003e\n\n\u003cul\u003e\n\u003cli\u003eEvaluation of epithelial morphology during primary cell expansion\u003c\/li\u003e\n\u003cli\u003eStudies of cell attachment and spreading\u003c\/li\u003e\n\u003cli\u003eAssessment of epithelial cell proliferation and survival\u003c\/li\u003e\n\u003cli\u003eResearch involving maintenance of epithelial characteristics in vitro\u003c\/li\u003e\n\u003cli\u003eComparison of culture conditions for tissue-derived epithelial cells\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eWhy Choose XR Epithelial Cell Growth Medium Kit?\u003c\/h2\u003e\n\n\u003cp\u003eSuccessful primary epithelial cell culture requires more than basic nutrient supplementation. Cells isolated from tissues may attach inefficiently, expand slowly or undergo early senescence when culture conditions are not adapted to epithelial cell requirements.\u003c\/p\u003e\n\n\u003cp\u003eXR Epithelial Cell Growth Medium Kit combines an optimized basal medium with selected epithelial supplements, recombinant human epidermal growth factor, transferrin and a controlled 2% serum concentration. This formulation is designed to provide sufficient growth support without relying on a conventional high-serum culture system.\u003c\/p\u003e\n\n\u003cp\u003eThe availability of separate EpiGM-B and EpiGM-M supplements allows researchers to select a culture configuration according to the epithelial cell source. This makes the system suitable for retinal pigment epithelial cells as well as respiratory, ocular surface, renal, mammary, prostate and sebaceous gland-derived epithelial cells.\u003c\/p\u003e\n\n\u003cul\u003e\n\u003cli\u003eDesigned specifically for primary epithelial cell culture rather than general-purpose cell maintenance\u003c\/li\u003e\n\u003cli\u003eSupports cell attachment, spreading, survival and proliferation\u003c\/li\u003e\n\u003cli\u003eLow-serum formulation containing 2% v\/v fetal bovine serum\u003c\/li\u003e\n\u003cli\u003eContains recombinant human EGF and transferrin\u003c\/li\u003e\n\u003cli\u003eSupports multiple tissue-derived epithelial cell types\u003c\/li\u003e\n\u003cli\u003eIncludes separate supplement options for RPE cells and other epithelial cells\u003c\/li\u003e\n\u003cli\u003eLow endotoxin and \u0026lt;0.2 μm filtered formulation\u003c\/li\u003e\n\u003cli\u003eSupplied without antibiotics or antifungal agents\u003c\/li\u003e\n\u003cli\u003eSuitable for complete-bottle or partial-volume preparation\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat is XR Epithelial Cell Growth Medium Kit used for?\u003c\/summary\u003e\n\u003cp\u003eXR Epithelial Cell Growth Medium Kit is used for the in vitro culture and expansion of primary epithelial cells. It is designed to support cell attachment, spreading, survival, proliferation and epithelial phenotype maintenance.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat is primary epithelial cell growth medium?\u003c\/summary\u003e\n\u003cp\u003ePrimary epithelial cell growth medium is a specialized culture formulation that provides nutrients and growth-supporting components for epithelial cells isolated from tissues. Unlike a general-purpose medium, it is designed around the attachment, proliferation and phenotype requirements of primary epithelial cells.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eIs XR Epithelial Cell Growth Medium serum-free?\u003c\/summary\u003e\n\u003cp\u003eNo. This product is a low-serum epithelial cell culture medium containing 2% v\/v fetal bovine serum. It should not be marketed or described as serum-free.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhich supplement should be used for retinal pigment epithelial cells?\u003c\/summary\u003e\n\u003cp\u003eRetinal pigment epithelial cells should be cultured using Epi basal medium combined with EpiGM-B supplement.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhich supplement should be used for bronchial or alveolar epithelial cells?\u003c\/summary\u003e\n\u003cp\u003eBronchial and alveolar epithelial cells should be cultured using Epi basal medium combined with EpiGM-M supplement.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eCan the medium be used for corneal epithelial cell culture?\u003c\/summary\u003e\n\u003cp\u003eYes. Epi basal medium combined with EpiGM-M supplement is intended for corneal epithelial cell culture. The same configuration can also be used for conjunctival epithelial cells.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eCan the medium be used for renal proximal tubule epithelial cells?\u003c\/summary\u003e\n\u003cp\u003eYes. The Epi basal medium and EpiGM-M supplement configuration is suitable for renal epithelial cells and renal proximal tubule epithelial cells.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eCan the medium be used for mammary and prostate epithelial cells?\u003c\/summary\u003e\n\u003cp\u003eYes. EpiGM-M complete medium is intended for primary mammary epithelial cells and prostate epithelial cells, as well as several other tissue-derived epithelial cell types.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eDoes the medium contain epidermal growth factor?\u003c\/summary\u003e\n\u003cp\u003eYes. The formulation contains recombinant human epidermal growth factor to support epithelial cell proliferation and culture performance.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eDoes the medium contain transferrin?\u003c\/summary\u003e\n\u003cp\u003eYes. Transferrin is included as part of the epithelial cell growth-supporting formulation.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eDoes XR Epithelial Cell Growth Medium contain antibiotics?\u003c\/summary\u003e\n\u003cp\u003eNo. The supplied product does not contain antibiotics or antifungal agents. Laboratories may add an antibiotic solution at 0.5–1% when required by their protocol.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eHow should the EpiGM supplement be thawed?\u003c\/summary\u003e\n\u003cp\u003eThaw the supplement at 4°C overnight or at room temperature for approximately 1–2 hours before mixing it with the basal medium.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the recommended mixing ratio for partial medium preparation?\u003c\/summary\u003e\n\u003cp\u003eFor partial preparation, combine 4 mL of EpiGM supplement with 96 mL of Epi basal medium to prepare 100 mL of complete epithelial cell growth medium.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eHow long can the prepared complete medium be stored?\u003c\/summary\u003e\n\u003cp\u003eThe prepared complete medium can be stored at 2–8°C for up to four weeks.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat seeding density is recommended for rapid epithelial cell growth?\u003c\/summary\u003e\n\u003cp\u003eFor rapid growth, a seeding density of approximately 10,000–15,000 cells\/cm² is recommended. Change the medium every other day and passage the cells at approximately 70–80% confluence.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat seeding density is recommended for routine passage?\u003c\/summary\u003e\n\u003cp\u003eFor routine passage, a seeding density of approximately 5,000–8,000 cells\/cm² is recommended. Change the medium every other day until the culture reaches approximately 70–80% confluence.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat are the storage conditions for the kit components?\u003c\/summary\u003e\n\u003cp\u003eStore the Epi basal medium at 2–8°C protected from light. Store EpiGM-M and EpiGM-B supplements at -20°C protected from light.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the endotoxin specification?\u003c\/summary\u003e\n\u003cp\u003eThe endotoxin specification is ≤0.1 EU\/mL.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eIs this product intended for clinical use?\u003c\/summary\u003e\n\u003cp\u003eNo. XR Epithelial Cell Growth Medium Kit is intended for research use only and is not intended for diagnostic, therapeutic or clinical applications.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003c\/div\u003e","brand":"XRbio","offers":[{"title":"1Kit","offer_id":52805119639733,"sku":"XREpiGM-M-500","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_fc827c94-0f1a-4adb-85f4-ea812db3dd59.jpg?v=1784104726","url":"https:\/\/biofargo.com\/products\/xr-human-epithelial-cell-medium-kit","provider":"Biofargo","version":"1.0","type":"link"}