{"product_id":"xr-human-cholangiocarcinoma-organoid-medium-kit","title":"XR Human Cholangiocarcinoma Organoid Medium Kit","description":"\u003c!--\nSEO TITLE:\nHuman Cholangiocarcinoma Organoid Culture Kit | PDO Medium\n\nMETA DESCRIPTION:\nEstablishing patient-derived cholangiocarcinoma organoids can be difficult. This complete culture kit supports reliable initiation, expansion, and passaging.\n\nRECOMMENDED URL:\n\/products\/human-cholangiocarcinoma-organoid-culture-kit\n\nPRIMARY KEYWORDS:\nHuman Cholangiocarcinoma Organoid Culture Kit\nCholangiocarcinoma Organoid Culture\nHuman Cholangiocarcinoma Organoid\nCholangiocarcinoma Organoid Medium\nPatient-Derived Cholangiocarcinoma Organoid\n\nSECONDARY KEYWORDS:\nCholangiocarcinoma Organoid Kit\nCholangiocarcinoma PDO\nPatient-Derived Organoid\nHuman Tumor Organoid Culture\nCancer Organoid Culture\n3D Tumor Organoid Culture\nOrganoid Expansion Medium\nLong-Term Organoid Culture\nOrganoid Maintenance\nOrganoid Passaging\nPrimary Tumor Organoid\nDrug Screening\nDrug Sensitivity Testing\nCancer Disease Modeling\nPrecision Oncology Research\nTumor Biology Research\n--\u003e\n\u003cstyle\u003e\n  \/* 现代专业风全局样式 *\/\n  .pdp-container {\n    font-family: -apple-system, BlinkMacSystemFont, \"Segoe UI\", Roboto, Helvetica, Arial, sans-serif;\n    color: 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class=\"pdp-container\"\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eEstablishing patient-derived cholangiocarcinoma organoids from primary tumor tissue can be technically demanding. Variable tissue quality, incomplete tissue digestion, inconsistent culture conditions, and limited post-isolation expansion may reduce organoid establishment efficiency and make long-term experiments difficult to reproduce.\u003c\/p\u003e\n\u003cp\u003eXR Human Cholangiocarcinoma Organoid Culture Kit is a complete in vitro culture system developed for the establishment, maintenance, and passaging of human cholangiocarcinoma organoids. The culture environment is designed to support three-dimensional growth while helping preserve relevant pathological and biological characteristics of the original tumor tissue.\u003c\/p\u003e\n\u003cp\u003eThis cholangiocarcinoma organoid medium is suitable for researchers constructing patient-derived cholangiocarcinoma organoid models from freshly collected tumor specimens. It supports the culture of multiple cholangiocarcinoma subtypes and provides a practical platform for tumor biology research, cancer disease modeling, drug screening, and drug sensitivity testing.\u003c\/p\u003e\n\u003cp\u003eThe two-component format combines 96 mL of Human Cholangiocarcinoma Organoid Basal Medium A with 4 mL of Human Cholangiocarcinoma Organoid Culture Supplement B to prepare 100 mL of complete medium. The integrated formulation reduces the need to source and optimize multiple individual culture factors.\u003c\/p\u003e\n\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cdiv class=\"pdp-table-responsive\"\u003e\n\u003ctable class=\"pdp-table\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eXR Human Cholangiocarcinoma Organoid Culture Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eXRhChlgCO-100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eProduct Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman cholangiocarcinoma organoid culture medium kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTarget Model\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePatient-derived human cholangiocarcinoma organoids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBasal Medium A\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e96 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eCulture Supplement B\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComplete Medium Volume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e100 mL after combining Basal Medium A and Supplement B\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eCulture Format\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThree-dimensional extracellular matrix-based organoid culture\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended Culture Conditions\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e37°C, 5% CO₂\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended Medium Volume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e700 μL per well in a 24-well plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBasal Medium Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4°C for up to 3 months or -20°C for up to 12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSupplement Storage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e-20°C for up to 6 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComponents Sold Separately\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNo\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eGrade\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eResearch use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003cdiv class=\"pdp-table-responsive\"\u003e\n\u003ctable class=\"pdp-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eVolume\u003c\/th\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003cth\u003eFunction\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman Cholangiocarcinoma Organoid Basal Medium A\u003c\/td\u003e\n\u003ctd\u003e96 mL\u003c\/td\u003e\n\u003ctd\u003e4°C or -20°C\u003c\/td\u003e\n\u003ctd\u003eProvides the basal nutritional environment for cholangiocarcinoma organoid culture.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHuman Cholangiocarcinoma Organoid Culture Supplement B\u003c\/td\u003e\n\u003ctd\u003e4 mL\u003c\/td\u003e\n\u003ctd\u003e-20°C\u003c\/td\u003e\n\u003ctd\u003eSupplies the concentrated culture supplement required to prepare complete medium.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"pdp-note\"\u003e\n\u003cp\u003e\u003cstrong\u003eComplete medium preparation:\u003c\/strong\u003e Add the entire 4 mL bottle of Culture Supplement B to 96 mL of Basal Medium A and mix thoroughly to prepare 100 mL of complete human cholangiocarcinoma organoid culture medium.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eDeveloped specifically for patient-derived human cholangiocarcinoma organoid culture.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSupports organoid establishment from freshly isolated primary cholangiocarcinoma tissue.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eDesigned for long-term organoid maintenance and serial passaging.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSupports research involving multiple cholangiocarcinoma subtypes.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eTwo-component format simplifies preparation of complete organoid growth medium.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eIntegrated culture formulation eliminates the need to add and optimize multiple individual factors.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSupports rapid organoid growth under appropriate tissue isolation and culture conditions.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eCan maintain organoid expansion for at least three passages, with some organoid cultures capable of expansion beyond ten passages.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eConsistent component formulation helps reduce batch-to-batch variation and improve experimental reproducibility.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSuitable for 3D tumor organoid culture, cancer biology research, and preclinical drug-response workflows.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eRecommended Culture Workflow\u003c\/h2\u003e\n\u003ch3\u003e1. Primary Tumor Tissue Collection\u003c\/h3\u003e\n\u003cp\u003eImmerse freshly collected cholangiocarcinoma tissue in pre-chilled primary tissue preservation solution and store temporarily at 4°C. Process the tissue as soon as practical to help preserve viable tumor cells.\u003c\/p\u003e\n\u003ch3\u003e2. Tissue Washing\u003c\/h3\u003e\n\u003cp\u003eUnder aseptic conditions, transfer the tissue to a culture dish and wash with pre-chilled PBS at least three times to reduce residual blood and tissue debris.\u003c\/p\u003e\n\u003ch3\u003e3. Mechanical Mincing\u003c\/h3\u003e\n\u003cp\u003eTransfer the washed tissue to a sterile 1.5 mL centrifuge tube and cut it into approximately 1–3 mm³ fragments using sterile surgical scissors.\u003c\/p\u003e\n\u003ch3\u003e4. Enzymatic Digestion\u003c\/h3\u003e\n\u003cp\u003eTransfer the tissue fragments to a 15 mL centrifuge tube and add 10 mL of primary tissue digestion solution. Digest at 37°C for approximately 15–45 minutes, depending on tissue source, size, and digestion progress. Gently pipette or agitate every 10 minutes while monitoring the tissue.\u003c\/p\u003e\n\u003ch3\u003e5. Filtration and Cell Collection\u003c\/h3\u003e\n\u003cp\u003eStop digestion with an appropriate neutralization solution. Pass the tissue suspension through a 100 μm cell strainer to remove undigested fragments, and centrifuge to collect the cell pellet.\u003c\/p\u003e\n\u003ch3\u003e6. Optional Red Blood Cell Lysis\u003c\/h3\u003e\n\u003cp\u003eWhen the collected pellet contains substantial red blood cell contamination, resuspend it in 1–2 mL of red blood cell lysis solution and incubate for approximately 2 minutes at room temperature. Stop the lysis, centrifuge, discard the supernatant, and retain the cell pellet.\u003c\/p\u003e\n\u003ch3\u003e7. Extracellular Matrix Embedding\u003c\/h3\u003e\n\u003cp\u003eResuspend the isolated cells in an appropriate extracellular matrix at a recommended density of 1 × 10\u003csup\u003e5\u003c\/sup\u003e to 1 × 10\u003csup\u003e6\u003c\/sup\u003e cells\/mL. Dispense the cell–matrix mixture into a 24-well plate while avoiding bubbles. Incubate at 37°C for approximately 15–25 minutes to allow the matrix domes to solidify.\u003c\/p\u003e\n\u003ch3\u003e8. Organoid Culture\u003c\/h3\u003e\n\u003cp\u003eAfter the matrix has solidified, carefully add 700 μL of complete human cholangiocarcinoma organoid medium around each matrix dome. Incubate the cultures at 37°C in a humidified atmosphere containing 5% CO₂.\u003c\/p\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eXR Human Cholangiocarcinoma Organoid Culture Kit provides a specialized platform for generating and expanding patient-derived tumor organoids that can be used in experimental workflows requiring a three-dimensional model of human cholangiocarcinoma.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003ePatient-derived cholangiocarcinoma organoid establishment:\u003c\/strong\u003e Construction of 3D organoid cultures from freshly isolated primary cholangiocarcinoma tissue.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCholangiocarcinoma PDO expansion:\u003c\/strong\u003e Maintenance and serial passaging of patient-derived organoids for extended experimental use.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCholangiocarcinoma disease modeling:\u003c\/strong\u003e Development of human tumor organoid models for studying biliary tract cancer biology in vitro.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eTumor heterogeneity research:\u003c\/strong\u003e Investigation of biological variation among organoids derived from different patients, tumor regions, or cholangiocarcinoma subtypes.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eDrug screening:\u003c\/strong\u003e Evaluation of candidate compounds in a patient-derived 3D tumor culture system.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eDrug sensitivity testing:\u003c\/strong\u003e Comparison of organoid responses to experimental therapeutic agents or treatment combinations.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision oncology research:\u003c\/strong\u003e Research workflows examining patient-specific tumor responses using cholangiocarcinoma PDO models.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCancer signaling research:\u003c\/strong\u003e Analysis of molecular pathways associated with cholangiocarcinoma growth, survival, and treatment response.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eGenetic and functional studies:\u003c\/strong\u003e Investigation of gene function and tumor-associated molecular changes in organoid-based experimental models.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBiomarker research:\u003c\/strong\u003e Study of candidate biomarkers associated with tumor subtype, progression, or drug response.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eTranslational tumor biology:\u003c\/strong\u003e Use of primary tumor organoids as an intermediate model between conventional cell culture and more complex in vivo studies.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eWhy Use a Cholangiocarcinoma Organoid Model?\u003c\/h2\u003e\n\u003cp\u003eConventional two-dimensional cancer cell lines are convenient for routine experiments but may not adequately reproduce the three-dimensional organization and biological diversity of primary cholangiocarcinoma tissue. Patient-derived organoids provide a complementary model that can retain clinically relevant tumor characteristics while remaining accessible to controlled in vitro experimentation.\u003c\/p\u003e\n\u003cp\u003eA cholangiocarcinoma organoid model can therefore support studies that require patient-specific tumor material, repeated sampling, long-term expansion, or parallel testing of multiple experimental conditions. When established successfully, the organoids can be used to connect primary tumor biology with downstream drug-response, biomarker, and disease-modeling workflows.\u003c\/p\u003e\n\u003ch2\u003eWhy Choose XR Human Cholangiocarcinoma Organoid Culture Kit?\u003c\/h2\u003e\n\u003cp\u003ePrimary cholangiocarcinoma specimens can differ substantially in cellularity, fibrosis, necrosis, and previous treatment exposure. These factors may influence tissue digestion, organoid formation, and subsequent expansion. A dedicated culture system helps reduce the additional variability introduced by assembling multiple independently sourced culture components.\u003c\/p\u003e\n\u003cp\u003eXR Human Cholangiocarcinoma Organoid Culture Kit provides a ready-to-prepare, two-component medium designed specifically for human cholangiocarcinoma organoid culture. The formulation supports establishment, maintenance, and passaging within a consistent workflow, allowing researchers to focus on tissue quality, matrix handling, and experimental design rather than repeated medium optimization.\u003c\/p\u003e\n\u003ch2\u003eHandling and Storage\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003ePrepare complete medium by mixing 96 mL of Basal Medium A with 4 mL of Culture Supplement B.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eMix thoroughly and keep the prepared complete medium on ice during initial experimental setup.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eWhen the complete medium will not be used at once, divide it into appropriate working aliquots.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAvoid repeated freeze–thaw cycles.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003ePerform medium preparation, aliquoting, and cell culture procedures under aseptic conditions.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eStore each component according to the conditions listed in the product specifications.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eUse only for laboratory research. This product is not intended for clinical diagnosis or therapeutic use.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the XR Human Cholangiocarcinoma Organoid Culture Kit used for?\u003c\/summary\u003e\n\u003cp\u003eThe kit is designed for the establishment, maintenance, and passaging of patient-derived human cholangiocarcinoma organoids. It provides a complete culture medium for 3D tumor organoid research using cells isolated from primary cholangiocarcinoma tissue.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is included in the cholangiocarcinoma organoid culture kit?\u003c\/summary\u003e\n\u003cp\u003eThe kit contains 96 mL of Human Cholangiocarcinoma Organoid Basal Medium A and 4 mL of Human Cholangiocarcinoma Organoid Culture Supplement B. The two components are mixed to prepare 100 mL of complete organoid culture medium.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan the basal medium and supplement be purchased separately?\u003c\/summary\u003e\n\u003cp\u003eNo. Basal Medium A and Culture Supplement B are supplied together as one complete human cholangiocarcinoma organoid culture kit and are not sold separately.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eDoes the kit support patient-derived cholangiocarcinoma organoids?\u003c\/summary\u003e\n\u003cp\u003eYes. The culture system is intended for establishing and expanding patient-derived cholangiocarcinoma organoids from primary tumor tissue. Successful establishment also depends on tissue quality, sample handling, digestion conditions, viable cell recovery, and extracellular matrix performance.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat types of cholangiocarcinoma can be studied with this medium?\u003c\/summary\u003e\n\u003cp\u003eThe product is designed to support research involving multiple cholangiocarcinoma subtypes. Because primary tumor samples can vary significantly, culture performance should be evaluated for each tissue source and experimental model.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan this cholangiocarcinoma organoid medium support long-term culture?\u003c\/summary\u003e\n\u003cp\u003eThe medium is designed for long-term maintenance and serial passaging. Product evaluation indicates support for at least three passages, while some organoid cultures may be expanded for more than ten passages under suitable conditions.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow is the complete cholangiocarcinoma organoid medium prepared?\u003c\/summary\u003e\n\u003cp\u003eAdd the entire 4 mL volume of Culture Supplement B to the 96 mL bottle of Basal Medium A. Mix thoroughly to obtain 100 mL of complete human cholangiocarcinoma organoid medium.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat cell density is recommended for extracellular matrix embedding?\u003c\/summary\u003e\n\u003cp\u003eThe recommended starting density is approximately 1 × 10\u003csup\u003e5\u003c\/sup\u003e to 1 × 10\u003csup\u003e6\u003c\/sup\u003e cells\/mL of extracellular matrix. The optimal density may vary according to sample viability, tumor cellularity, and the objective of the experiment.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow much complete medium should be added to each well?\u003c\/summary\u003e\n\u003cp\u003eFor the described 24-well plate workflow, add approximately 700 μL of complete cholangiocarcinoma organoid medium around each solidified extracellular matrix dome.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan this kit be used for cholangiocarcinoma drug screening?\u003c\/summary\u003e\n\u003cp\u003eYes. Patient-derived cholangiocarcinoma organoids generated with the kit may be used as 3D tumor models in research workflows involving compound screening, drug sensitivity testing, combination-treatment evaluation, and precision oncology research.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eDoes the kit include tissue digestion solution, PBS, extracellular matrix, or red blood cell lysis solution?\u003c\/summary\u003e\n\u003cp\u003eThe listed kit components are Basal Medium A and Culture Supplement B. Tissue preservation solution, PBS, digestion and neutralization solutions, cell strainers, red blood cell lysis reagents, extracellular matrix, culture plates, and other tissue-processing materials should be prepared separately.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow should the kit components be stored?\u003c\/summary\u003e\n\u003cp\u003eBasal Medium A may be stored at 4°C for up to 3 months or at -20°C for up to 12 months. Culture Supplement B should be stored at -20°C for up to 6 months. Avoid repeated freeze–thaw cycles by preparing suitable aliquots when necessary.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eIs this product intended for clinical use?\u003c\/summary\u003e\n\u003cp\u003eNo. XR Human Cholangiocarcinoma Organoid Culture Kit is intended for research use only and must not be used for clinical diagnosis, human treatment, or therapeutic procedures.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003c\/div\u003e\n","brand":"XRbio","offers":[{"title":"1Kit","offer_id":52798001283253,"sku":"XRhChlgCO-100","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_02ae0975-c919-4777-85cc-65da6bc778cd.jpg?v=1783998029","url":"https:\/\/biofargo.com\/products\/xr-human-cholangiocarcinoma-organoid-medium-kit","provider":"Biofargo","version":"1.0","type":"link"}