{"product_id":"xr-hipsc-suspension-culture-medium","title":"XR hES\/iPSC Suspension Culture Medium Kit (AOF) hES\/iPSC","description":"\u003cstyle\u003e\n.pdp-container {\n  font-family: -apple-system, BlinkMacSystemFont, \"Segoe UI\", Roboto, Helvetica, Arial, sans-serif;\n  color:#333333;\n  line-height:1.6;\n  max-width:1200px;\n  margin:0 auto;\n  padding:15px;\n}\n\n.pdp-container h2{\n  font-size:24px;\n  color:#0B3C5D;\n  border-bottom:2px solid #328CC1;\n  padding-bottom:8px;\n  margin-top:40px;\n  margin-bottom:20px;\n  font-weight:600;\n}\n\n.pdp-container h3{\n  font-size:18px;\n  color:#1D2731;\n  margin-top:30px;\n  margin-bottom:15px;\n}\n\n.pdp-container p{\n  margin-bottom:16px;\n  font-size:15px;\n  color:#4A4A4A;\n  text-align:justify;\n}\n\n.pdp-container ul{\n  padding-left:20px;\n  margin-bottom:24px;\n}\n\n.pdp-container li{\n  margin-bottom:10px;\n  font-size:15px;\n  list-style-type:square;\n}\n\n.pdp-table-responsive{\n  width:100%;\n  overflow-x:auto;\n  margin:25px 0;\n}\n\n.pdp-table{\n  width:100%;\n  border-collapse:collapse;\n  min-width:650px;\n}\n\n.pdp-table td{\n  padding:12px 16px;\n  border-bottom:1px solid #E0E0E0;\n  font-size:14px;\n}\n\n.pdp-table tr:nth-child(even){\n  background:#F8F9FA;\n}\n\n.pdp-img-container{\n  max-width:650px;\n  margin:25px auto;\n  padding:10px;\n  border:1px solid #EEEEEE;\n  border-radius:8px;\n  background:#fff;\n  text-align:center;\n}\n\n.pdp-img-container img{\n  max-width:100%;\n  height:auto;\n}\n\n.pdp-container details{\n  background:#F8F9FA;\n  border:1px solid #E0E0E0;\n  border-radius:6px;\n  padding:14px 18px;\n  margin-bottom:12px;\n}\n\n.pdp-container summary{\n  font-weight:600;\n  color:#0B3C5D;\n  cursor:pointer;\n}\n\u003c\/style\u003e\n\u003cdiv class=\"pdp-container\"\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eScaling human pluripotent stem cell (hPSC) culture from routine research workflows to larger expansion systems remains challenging when using conventional adherent culture methods. Limited surface area, dependence on extracellular matrix coating, manual passaging steps, and difficulties maintaining consistent cell aggregates can affect expansion efficiency and downstream applications.\u003c\/p\u003e\n\u003cp\u003eXR hES\/iPSC Suspension Culture Medium Kit (AOF) is an animal-origin-free (AOF) human pluripotent stem cell culture medium designed for feeder-free suspension expansion of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs). This iPSC suspension culture medium enables the formation of uniform cell aggregates, supporting scalable 3D stem cell culture workflows without the requirement for microcarriers.\u003c\/p\u003e\n\u003cp\u003eDesigned for researchers developing scalable hPSC expansion workflows, XR hES\/iPSC Suspension Culture Medium Kit supports suspension-based iPSC expansion, spheroid formation, and downstream differentiation applications including organoid research. The system provides a flexible solution for transitioning between suspension (3D) and adherent (2D) culture formats.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cdiv class=\"pdp-table-responsive\"\u003e\n\u003ctable class=\"pdp-table\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eXR hES\/iPSC Suspension Culture Medium Kit (AOF)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eXRiPSC-sus-500\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eProduct Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHuman pluripotent stem cell suspension culture medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplication Format\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e3D suspension culture of hESC and iPSC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAnimal-origin-free (AOF)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBasal Medium\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e496 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eAdditive\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eFiltration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026lt;0.2 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eEndotoxin\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e≤0.125 EU\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBacteria Detection\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNot detected\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eFungi Detection\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNot detected\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eBasal medium: 2–8°C\u003cbr\u003eAdditive: -20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eShelf Life\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eFeeder-free iPSC suspension culture medium designed for human pluripotent stem cell expansion.\u003c\/li\u003e\n\u003cli\u003eAnimal-origin-free formulation suitable for defined stem cell culture workflows.\u003c\/li\u003e\n\u003cli\u003eSupports microcarrier-free 3D suspension culture of hESC and iPSC.\u003c\/li\u003e\n\u003cli\u003ePromotes formation of relatively uniform cell aggregates mainly distributed around 200–300 μm.\u003c\/li\u003e\n\u003cli\u003eEnables transition between suspension culture and adherent culture workflows.\u003c\/li\u003e\n\u003cli\u003eSupports scalable hPSC expansion from multi-well plates toward bioreactor-based culture systems.\u003c\/li\u003e\n\u003cli\u003eDesigned for downstream 3D differentiation and organoid research applications.\u003c\/li\u003e\n\u003cli\u003eFiltered using a \u0026lt;0.2 μm filtration process for consistent culture preparation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003ch3\u003eSuspension Expansion of Human iPSC\u003c\/h3\u003e\n\u003cp\u003eXR hES\/iPSC Suspension Culture Medium Kit was evaluated using human iPSC suspension culture workflows. In ultra-low attachment 6-well plates, iPSC aggregates were expanded under orbital shaking conditions, demonstrating efficient suspension-based cell expansion.\u003c\/p\u003e\n\u003cdiv class=\"pdp-img-container\" style=\"text-align: center;\"\u003e\n\u003c!-- Image Placeholder --\u003e \u003cimg alt=\"Human iPSC suspension expansion performance using XR hES\/iPSC suspension culture medium in ultra-low attachment plates\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_03d9cb17-f5b9-4ba4-8172-b1e58855e566.jpg?v=1783672177\" style=\"margin-bottom: 16px; float: none;\"\u003e\n\u003c\/div\u003e\n\u003cp\u003eIn a disposable bioreactor-based suspension culture workflow, XR hES\/iPSC Suspension Culture Medium supported scalable expansion conditions suitable for larger-volume hPSC culture evaluation.\u003c\/p\u003e\n\u003cdiv class=\"pdp-img-container\" style=\"text-align: center;\"\u003e\u003cimg alt=\"Bioreactor scale-up suspension culture of human iPSC using XR stem cell expansion medium\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/2_7e43fa6e-315e-4ce6-88e8-9451037d87fb.jpg?v=1783672177\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003ch2\u003eUniform iPSC Aggregate Formation\u003c\/h2\u003e\n\u003cp\u003eSuspension culture with XR medium enables formation of three-dimensional human pluripotent stem cell aggregates. Uniform aggregate size distribution can help support consistent downstream differentiation workflows.\u003c\/p\u003e\n\u003cdiv class=\"pdp-img-container\" style=\"text-align: center;\"\u003e\u003cimg alt=\"Uniform iPSC spheroid aggregate formation during 3D suspension stem cell culture\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_4e731db2-ef12-488b-b44b-c904d7a5d894.jpg?v=1783672333\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003ch2\u003eMaintenance of Pluripotent Characteristics\u003c\/h2\u003e\n\u003cp\u003eLong-term suspension culture evaluation demonstrated maintenance of commonly used pluripotency markers including SSEA-3 and TRA-1-81 in human iPSC and embryonic stem cell cultures.\u003c\/p\u003e\n\u003cdiv class=\"pdp-img-container\" style=\"text-align: center;\"\u003e\u003cimg alt=\"SSEA-3 and TRA-1-81 pluripotency marker analysis of hPSC suspension culture\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_e5fcd4fe-2a80-4f73-aa90-e0a6441e8b03.jpg?v=1783672484\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eXR hES\/iPSC Suspension Culture Medium Kit is designed for researchers requiring scalable and reproducible human pluripotent stem cell expansion solutions. The suspension culture format supports applications where traditional adherent workflows may become limiting.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eHuman induced pluripotent stem cell (iPSC) suspension expansion\u003c\/li\u003e\n\u003cli\u003eHuman embryonic stem cell (hESC) 3D suspension culture\u003c\/li\u003e\n\u003cli\u003eFeeder-free pluripotent stem cell culture workflows\u003c\/li\u003e\n\u003cli\u003eLarge-scale hPSC expansion research\u003c\/li\u003e\n\u003cli\u003e3D stem cell aggregate and spheroid culture\u003c\/li\u003e\n\u003cli\u003eOrganoid differentiation research using iPSC-derived cells\u003c\/li\u003e\n\u003cli\u003eStem cell bioprocess development studies\u003c\/li\u003e\n\u003cli\u003eBioreactor-compatible suspension culture research\u003c\/li\u003e\n\u003cli\u003eHuman pluripotent stem cell characterization studies\u003c\/li\u003e\n\u003cli\u003eRegenerative medicine and disease modeling research\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eWhy Choose XR hES\/iPSC Suspension Culture Medium?\u003c\/h2\u003e\n\u003cp\u003eResearchers working with human pluripotent stem cells increasingly require culture systems that support scalability, consistency, and compatibility with advanced 3D biology workflows. Suspension-based hPSC expansion provides an alternative approach when conventional adherent culture methods become difficult to scale.\u003c\/p\u003e\n\u003cp\u003eXR hES\/iPSC Suspension Culture Medium Kit combines feeder-free suspension expansion, animal-origin-free formulation, and 3D aggregate culture capability to support modern iPSC research workflows including organoid development and scalable stem cell culture studies.\u003c\/p\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is XR hES\/iPSC Suspension Culture Medium Kit used for?\u003c\/summary\u003e\n\u003cp\u003eThis product is a human pluripotent stem cell suspension culture medium designed for expansion of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) in 3D suspension workflows.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the advantage of iPSC suspension culture compared with adherent culture?\u003c\/summary\u003e\n\u003cp\u003eSuspension culture can reduce dependence on surface coating materials and provide a scalable approach for generating human pluripotent stem cell aggregates.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eDoes this medium support feeder-free iPSC culture?\u003c\/summary\u003e\n\u003cp\u003eYes. XR hES\/iPSC Suspension Culture Medium Kit is designed for feeder-free hPSC expansion workflows.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat size cell aggregates are generated during suspension culture?\u003c\/summary\u003e\n\u003cp\u003eThe product supports formation of relatively uniform cell aggregates, mainly distributed around 200–300 μm according to evaluation data.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan XR hES\/iPSC Suspension Medium be used for organoid research?\u003c\/summary\u003e\n\u003cp\u003eYes. Suspension-cultured hPSC aggregates can be directly transitioned into 3D differentiation workflows including organoid research applications.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eIs this an animal-origin-free stem cell culture medium?\u003c\/summary\u003e\n\u003cp\u003eYes. XR hES\/iPSC Suspension Culture Medium Kit is designed as an animal-origin-free (AOF) culture system.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan this medium support bioreactor-based hPSC expansion?\u003c\/summary\u003e\n\u003cp\u003eThe product has been evaluated in disposable bioreactor suspension culture workflows, supporting scalable hPSC expansion research.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow should XR hES\/iPSC Suspension Culture Medium be stored?\u003c\/summary\u003e\n\u003cp\u003eThe basal medium should be stored at 2–8°C and the additive should be stored at -20°C.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003cscript type=\"application\/ld+json\"\u003e\n\n{\n\"@context\":\"https:\/\/schema.org\",\n\"@type\":\"Product\",\n\"name\":\"XR hES\/iPSC Suspension Culture Medium Kit (AOF)\",\n\"sku\":\"XRiPSC-sus-500\",\n\"description\":\"Animal-origin-free iPSC suspension culture medium designed for feeder-free 3D expansion of human pluripotent stem cells.\",\n\"category\":\"Stem Cell Culture Media\",\n\"brand\":{\n\"@type\":\"Brand\",\n\"name\":\"XR\"\n}\n}\n\n\u003c\/script\u003e \u003cscript type=\"application\/ld+json\"\u003e\n\n{\n\"@context\":\"https:\/\/schema.org\",\n\"@type\":\"FAQPage\",\n\"mainEntity\":[\n\n{\n\"@type\":\"Question\",\n\"name\":\"What is XR hES\/iPSC Suspension Culture Medium Kit used for?\",\n\"acceptedAnswer\":{\n\"@type\":\"Answer\",\n\"text\":\"It is designed for suspension expansion of human embryonic stem cells and induced pluripotent stem cells.\"\n}\n},\n\n{\n\"@type\":\"Question\",\n\"name\":\"Does XR medium support feeder-free iPSC culture?\",\n\"acceptedAnswer\":{\n\"@type\":\"Answer\",\n\"text\":\"Yes, the medium supports feeder-free hPSC suspension culture workflows.\"\n}\n},\n\n{\n\"@type\":\"Question\",\n\"name\":\"Can this medium support organoid research?\",\n\"acceptedAnswer\":{\n\"@type\":\"Answer\",\n\"text\":\"Yes, suspension cultured aggregates can transition into 3D differentiation workflows.\"\n}\n}\n\n]\n\n}\n\n\u003c\/script\u003e\u003c\/p\u003e","brand":"XRbio","offers":[{"title":"1Kit","offer_id":52784521478325,"sku":"XRiPSC-sus-500","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_8e0fe68f-7386-4ddb-94b2-abdec9e46c04.jpg?v=1783672620","url":"https:\/\/biofargo.com\/products\/xr-hipsc-suspension-culture-medium","provider":"Biofargo","version":"1.0","type":"link"}