Q: What is XR hFIB Max Human Dermal Fibroblast Medium?

XR hFIB Max is a serum-free human dermal fibroblast culture medium designed for expansion, maintenance, and continuous passage of human skin fibroblasts.

Q: What cells can be cultured with XR hFIB Max?

XR hFIB Max is designed for human dermal fibroblast culture including primary human dermal fibroblast expansion workflows.

Q: Is XR hFIB Max a serum-free fibroblast medium?

Yes. XR hFIB Max is a serum-free fibroblast culture medium that does not require fetal bovine serum supplementation.

Q: Can XR hFIB Max support primary human fibroblast expansion?

Yes. XR hFIB Max supports primary human dermal fibroblast expansion and maintenance applications.

Q: Is XR hFIB Max suitable for skin tissue engineering research?

Yes. XR hFIB Max supports fibroblast culture workflows used in skin tissue engineering and extracellular matrix research.

How should XR hFIB Max be stored?

Basal Medium A should be stored at 4°C and Supplement B should be stored at -20°C.

Description

Maintaining reliable expansion of primary human dermal fibroblasts can be challenging when using conventional serum-containing culture systems. Variations in fetal bovine serum (FBS) composition, undefined growth factors, and batch-to-batch differences may influence fibroblast proliferation, morphology consistency, and reproducibility between experiments.

XR hFIB Max Serum-Free Human Dermal Fibroblast Culture Medium is specifically designed for human dermal fibroblast culture and primary HDF expansion workflows. The optimized serum-free formulation provides a stable environment for human skin fibroblast growth while eliminating the variability associated with traditional serum supplementation.

Designed as a dedicated human dermal fibroblast medium, XR hFIB Max supports the isolation, expansion, and continuous passage of human dermal fibroblasts. The formulation contains optimized cellular support components, antioxidant ingredients, and culture stabilization factors to help researchers maintain consistent fibroblast growth performance for skin biology, tissue engineering, and extracellular matrix research applications.

XR hFIB Max provides researchers with a standardized serum-free fibroblast medium solution for applications requiring reliable primary fibroblast culture conditions, reduced experimental variability, and improved workflow consistency.

Specifications

Product Name	XR hFIB Max Serum-Free Human Dermal Fibroblast Culture Medium
Product Type	Human dermal fibroblast growth medium
Culture Application	Primary human dermal fibroblast expansion and maintenance
Components	Basal Medium A + Supplement B
Basal Medium A	475 mL/bottle
Supplement B	25 mL/bottle
Serum Content	Serum-free formulation
Animal Origin Components	No animal-derived components
pH	7.2–7.4
Endotoxin	≤0.1 EU/mL
Filtration	<0.2 μm filtration
Bacteria Detection	Not detected
Fungi Detection	Not detected
Storage	Basal Medium A: 4°C Supplement B: -20°C
Shelf Life	12 months
Research Grade	Research use only

Features

Optimized Human Dermal Fibroblast Medium — Specifically developed for human skin fibroblast culture, supporting primary HDF expansion and maintenance workflows.
Serum-Free Fibroblast Culture System — Eliminates dependence on fetal bovine serum and reduces variability caused by undefined serum components.
Supports Primary Fibroblast Expansion — Suitable for human dermal fibroblast isolation, expansion, and continuous passage applications.
Enhanced Culture Stability — Contains antioxidant components and culture stabilization factors to support consistent cell growth conditions.
Animal-Free Formulation — Provides a more standardized culture environment for researchers requiring controlled fibroblast culture workflows.
Reliable Cell Culture Performance — Designed to maintain fibroblast morphology and proliferation during routine culture.
Quality-Controlled Manufacturing — Filtered through a <0.2 μm filtration process with endotoxin and contamination testing.

Performance Data

Human Dermal Fibroblast Morphology During Culture

Maintaining consistent fibroblast morphology is an important factor in primary human dermal fibroblast culture. XR hFIB Max was evaluated for supporting human dermal fibroblast attachment, spreading, and proliferation during routine culture conditions.

The cultured human dermal fibroblasts showed progressive cell expansion and typical fibroblast-like morphology during the observation period. Cells gradually increased in density while maintaining the characteristic elongated spindle-shaped appearance associated with dermal fibroblast cultures.

Human dermal fibroblast morphology at 24 48 and 72 hours cultured in serum-free fibroblast medium XR hFIB Max

Representative microscopy images demonstrate human dermal fibroblast growth progression during culture with XR hFIB Max human dermal fibroblast culture medium.

Fibroblast Expansion Performance Compared with Conventional Serum Culture

Traditional fibroblast culture commonly relies on basal media supplemented with fetal bovine serum (FBS). However, serum-containing systems may introduce variability due to undefined components and lot-to-lot differences. XR hFIB Max was developed as a serum-free fibroblast medium to provide a more controlled environment for human fibroblast expansion.

According to comparative evaluation data, XR hFIB Max demonstrated enhanced expansion performance compared with conventional DMEM/F12 supplemented with 10% FBS under tested culture conditions.

Human dermal fibroblast doubling time comparison between serum-free fibroblast medium XR hFIB Max and DMEM F12 with FBS

The comparison analysis highlights the ability of XR hFIB Max to support efficient primary fibroblast expansion while providing a defined serum-free culture environment.

Stable Culture Environment for Primary Human Fibroblast Expansion

Primary human fibroblast cultures can be sensitive to changes in nutrient availability, oxidative stress, and culture conditions. XR hFIB Max incorporates optimized culture-support components and antioxidant factors to improve environmental stability during fibroblast expansion workflows.

Supports consistent proliferation of human dermal fibroblasts during routine culture.
Provides a standardized serum-free culture system for reduced experimental variation.
Suitable for researchers performing primary fibroblast culture and repeated passage experiments.

Applications

XR hFIB Max Serum-Free Human Dermal Fibroblast Culture Medium is designed for researchers requiring reliable human dermal fibroblast expansion and maintenance conditions. The optimized formulation supports applications involving primary fibroblast biology, skin research, extracellular matrix studies, and tissue engineering workflows.

Primary Human Dermal Fibroblast Culture

XR hFIB Max is suitable for establishing and expanding primary human dermal fibroblast cultures after tissue isolation. The serum-free formulation helps researchers maintain controlled culture conditions for downstream analysis.

Primary human dermal fibroblast isolation and expansion
Human skin fibroblast culture and maintenance
Dermal fibroblast proliferation studies
Human primary cell culture research workflows

Skin Tissue Engineering Research

Dermal fibroblasts play important roles in extracellular matrix production, collagen synthesis, and skin structure maintenance. XR hFIB Max provides a dedicated fibroblast expansion medium for researchers developing in vitro skin models and tissue engineering platforms.

Skin tissue engineering studies
Human skin model development
Dermal regeneration research
Cell-based skin biology studies

Extracellular Matrix and Collagen Research

Human dermal fibroblasts are widely used for studying extracellular matrix (ECM) remodeling and collagen-related biological processes. XR hFIB Max supports fibroblast culture workflows used in ECM analysis and connective tissue research.

Extracellular matrix research
Collagen production studies
Fibroblast-mediated matrix remodeling research
Cell signaling studies involving dermal fibroblasts

Regenerative Medicine and Cell Biology Research

The ability to generate consistent fibroblast populations is important for regenerative biology research. XR hFIB Max provides a standardized serum-free fibroblast culture system for laboratories studying human fibroblast function and tissue repair mechanisms.

Wound healing research models
Regenerative medicine research
Fibroblast phenotype analysis
Human cell-based assay development

Why Choose XR hFIB Max Human Dermal Fibroblast Medium?

Human dermal fibroblast culture requires a reliable and reproducible environment to support cell expansion, morphology maintenance, and downstream functional studies. Conventional serum-containing fibroblast culture systems may introduce experimental variation caused by undefined serum components, inconsistent growth support, and differences between serum batches.

XR hFIB Max Serum-Free Human Dermal Fibroblast Culture Medium provides a dedicated culture solution designed for researchers working with primary human dermal fibroblasts. The optimized formulation eliminates the need for serum supplementation while supporting efficient fibroblast expansion and stable culture performance.

By combining serum-free formulation design with antioxidant components and culture stabilization factors, XR hFIB Max helps laboratories establish more controlled human fibroblast culture workflows for skin biology, extracellular matrix research, and tissue engineering applications.

Designed for Reliable HDF Culture Workflows

Reduced dependence on serum variability — Provides a defined serum-free environment compared with traditional FBS-containing fibroblast culture systems.
Optimized for human dermal fibroblasts — Developed specifically for human skin fibroblast expansion and maintenance applications.
Supports primary cell expansion — Suitable for researchers establishing and expanding primary human fibroblast cultures.
Compatible with skin research applications — Supports workflows involving dermal biology, ECM studies, and tissue engineering research.

Usage Guidelines

XR hFIB Max consists of two components: Basal Medium A and Supplement B. Before cell culture, Supplement B should be thawed at 4°C or room temperature and mixed with the basal medium according to the recommended preparation procedure.

Recommended Cell Type	Human dermal fibroblasts
Recommended Seeding Density	4,000–6,000 cells/cm²
Culture Temperature	37°C
CO₂ Condition	5% CO₂
Passage Recommendation	Perform passage when cells reach approximately 80–90% confluence
Prepared Medium Storage	Store prepared medium at 4°C for up to 2 weeks

For continuous serum-free fibroblast culture, gentle dissociation methods or 0.05% trypsin treatment are recommended. After digestion, dilute cells with at least two volumes of culture medium to minimize prolonged protease exposure.

FAQ

What is XR hFIB Max Human Dermal Fibroblast Medium?

XR hFIB Max is a serum-free human dermal fibroblast culture medium designed for the expansion, maintenance, and continuous passage of human skin fibroblasts. It provides a controlled culture environment without traditional serum supplementation.

What cells can be cultured with XR hFIB Max?

XR hFIB Max is designed for human dermal fibroblast culture, including primary human dermal fibroblast expansion and routine maintenance workflows.

Is XR hFIB Max a serum-free fibroblast medium?

Yes. XR hFIB Max is formulated as a serum-free fibroblast culture medium and does not require fetal bovine serum supplementation during routine culture.

What is the advantage of using serum-free fibroblast culture medium instead of FBS-based medium?

Serum-free fibroblast medium helps reduce variability associated with undefined serum components and batch differences. XR hFIB Max provides a more standardized environment for human dermal fibroblast expansion and research applications.

Can XR hFIB Max support primary human fibroblast expansion?

Yes. XR hFIB Max is designed for primary human dermal fibroblast expansion, supporting cell attachment, proliferation, and continuous culture workflows.

Is XR hFIB Max suitable for skin tissue engineering research?

Yes. Human dermal fibroblasts cultured with XR hFIB Max can be used in research workflows related to skin tissue engineering, extracellular matrix studies, collagen-related research, and dermal biology.

Does XR hFIB Max contain animal-derived components?

XR hFIB Max is designed as an animal-free formulation to provide a more controlled culture environment compared with traditional serum-containing fibroblast media.

How should XR hFIB Max components be stored?

The basal medium should be stored at 4°C, while Supplement B should be stored at -20°C. Supplement B is recommended to be aliquoted to minimize repeated freeze-thaw cycles.

Does XR hFIB Max contain antibiotics?

No. XR hFIB Max does not contain antibiotics. Antibiotic solution may be added according to individual laboratory requirements and experimental conditions.

XR hFIB Max - Human Fibroblast Medium Kit