Description

Western & IP Cell Lysis Buffer is a mild non-denaturing protein extraction buffer designed for researchers who need to preserve native protein structures and protein-protein interactions during cell lysis. During protein preparation, harsh detergents or denaturing conditions may disrupt protein complexes, alter antibody recognition sites, or reduce protein activity, which can affect immunoprecipitation and interaction studies. This IP-compatible cell lysis buffer provides gentle protein solubilization for reliable Western blot, immunoprecipitation (IP), and co-immunoprecipitation (Co-IP) workflows.

Formulated with a nonionic detergent system, Western & IP Cell Lysis Buffer efficiently disrupts cellular membranes while maintaining native protein conformation. The resulting whole-cell lysates contain soluble cytoplasmic proteins, soluble membrane-associated proteins, and nuclear proteins suitable for antibody-based enrichment and protein interaction analysis.

Compared with conventional strong lysis buffers, this non-denaturing cell lysis buffer is optimized for applications requiring preservation of protein complexes, antigen-antibody recognition, and native protein characteristics. It is an ideal protein extraction reagent for Western blot sample preparation, immunoprecipitation assays, and Co-IP experiments.

Specifications

Product Name	Western & IP Cell Lysis Buffer
SKU	RP0131
Volume	100 mL
Product Type	Non-denaturing Cell Lysis Buffer for Western Blot and Immunoprecipitation
Detergent Type	Nonionic detergent-based formulation
Applications	Western Blot, Immunoprecipitation (IP), Co-Immunoprecipitation (Co-IP), Protein Extraction
Sample Types	Cultured cells, suspension cells, mammalian tissues
Storage	4 °C
Shelf Life	18 months

Features

Non-denaturing protein extraction: Maintains native protein structures and antigen recognition sites for downstream antibody-based assays.
Designed for IP and Co-IP workflows: Helps preserve protein complexes and supports protein-protein interaction studies.
Gentle cell membrane disruption: Enables efficient release of soluble cellular proteins while minimizing protein complex disruption.
Compatible with Western blot analysis: Provides reliable whole-cell lysates for SDS-PAGE and immunoblotting workflows.
Broad sample compatibility: Suitable for adherent cells, suspension cells, and tissue protein extraction.

Applications

Western & IP Cell Lysis Buffer is widely used as a native protein extraction reagent for molecular biology, cell biology, and biochemical research applications where preservation of protein characteristics is required.

Western Blot Protein Analysis: Preparation of whole-cell lysates for SDS-PAGE separation and Western blot detection of target proteins.
Immunoprecipitation (IP): Provides compatible cell lysates for antibody-based protein enrichment while maintaining native antigen structures.
Co-Immunoprecipitation (Co-IP): Supports protein-protein interaction studies by preserving protein complexes during extraction.
Native Protein Extraction: Suitable for experiments requiring functional proteins or preserved protein conformations.
Protein Complex Analysis: Enables investigation of protein assemblies, signaling complexes, and molecular interactions.
Cell Signaling Research: Supports analysis of signaling pathway proteins where antibody recognition and protein integrity are important.
Antibody Binding Studies: Useful when antibodies recognize conformational epitopes that may be affected by denaturing extraction conditions.
Biochemical Research: Used for protein characterization, interaction analysis, and cellular protein studies.

Protocol Overview

For optimal protein extraction performance, sample preparation should be performed under cold conditions to minimize protein degradation and preserve native protein characteristics.

Add protease inhibitors such as PMSF immediately before use according to experimental requirements.
Add lysis buffer to prepared cell or tissue samples and incubate on ice for efficient protein extraction.
Centrifuge lysates at 10,000–14,000 ×g for 3–5 minutes to remove insoluble debris.
Collect the clarified supernatant for Western blot, IP, Co-IP, or other protein analysis workflows.

Because this buffer contains detergent components, BCA protein assays are recommended for protein concentration measurement instead of Bradford assays.

Ordering Information

SKU	Product	Size
RP0131	Western & IP Cell Lysis Buffer	100 mL

FAQ

What is Western & IP Cell Lysis Buffer used for?

Western & IP Cell Lysis Buffer is a non-denaturing cell lysis buffer designed for protein extraction before Western blot, immunoprecipitation (IP), and co-immunoprecipitation (Co-IP) experiments. It helps maintain native protein structures and protein interactions during sample preparation.

What is the difference between IP lysis buffer and RIPA buffer?

IP lysis buffer is optimized for maintaining protein complexes and native antigen structures, while RIPA buffer generally provides stronger protein solubilization. For protein-protein interaction studies and Co-IP experiments, a mild non-denaturing lysis buffer is usually preferred.

Can this cell lysis buffer be used for Western blot?

Yes. Western & IP Cell Lysis Buffer can be used for Western blot sample preparation. The extracted whole-cell lysates are compatible with SDS-PAGE and antibody-based protein detection workflows.

Why is a non-denaturing lysis buffer important for immunoprecipitation?

Non-denaturing lysis conditions help preserve protein conformation and protein complexes, allowing antibodies to recognize native epitopes and enabling more reliable immunoprecipitation results.

Can this buffer be used for Co-IP protein interaction studies?

Yes. This Co-IP compatible lysis buffer is designed to preserve protein-protein interactions and is suitable for studying protein complexes and cellular signaling interactions.

Which protein assay method is recommended after extraction?

BCA protein assays are recommended because detergent-containing lysis buffers may interfere with Bradford protein quantification methods.