{"product_id":"western-ip-cell-lysis-buffer","title":"Western \u0026 IP Cell Lysis Buffer","description":"\u003c!-- =========================\nPRODUCT DESCRIPTION\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eWestern \u0026amp; IP Cell Lysis Buffer is a mild non-denaturing protein extraction buffer designed for researchers who need to preserve native protein structures and protein-protein interactions during cell lysis. During protein preparation, harsh detergents or denaturing conditions may disrupt protein complexes, alter antibody recognition sites, or reduce protein activity, which can affect immunoprecipitation and interaction studies. This IP-compatible cell lysis buffer provides gentle protein solubilization for reliable Western blot, immunoprecipitation (IP), and co-immunoprecipitation (Co-IP) workflows.\u003c\/p\u003e\n\u003cp\u003eFormulated with a nonionic detergent system, Western \u0026amp; IP Cell Lysis Buffer efficiently disrupts cellular membranes while maintaining native protein conformation. The resulting whole-cell lysates contain soluble cytoplasmic proteins, soluble membrane-associated proteins, and nuclear proteins suitable for antibody-based enrichment and protein interaction analysis.\u003c\/p\u003e\n\u003cp\u003eCompared with conventional strong lysis buffers, this non-denaturing cell lysis buffer is optimized for applications requiring preservation of protein complexes, antigen-antibody recognition, and native protein characteristics. It is an ideal protein extraction reagent for Western blot sample preparation, immunoprecipitation assays, and Co-IP experiments.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nSPECIFICATIONS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"8\" border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eWestern \u0026amp; IP Cell Lysis Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eRP0131\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eVolume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eNon-denaturing Cell Lysis Buffer for Western Blot and Immunoprecipitation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eDetergent Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eNonionic detergent-based formulation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eWestern Blot, Immunoprecipitation (IP), Co-Immunoprecipitation (Co-IP), Protein Extraction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSample Types\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eCultured cells, suspension cells, mammalian tissues\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e4 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eShelf Life\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e18 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFEATURES\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eNon-denaturing protein extraction:\u003c\/strong\u003e Maintains native protein structures and antigen recognition sites for downstream antibody-based assays.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eDesigned for IP and Co-IP workflows:\u003c\/strong\u003e Helps preserve protein complexes and supports protein-protein interaction studies.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eGentle cell membrane disruption:\u003c\/strong\u003e Enables efficient release of soluble cellular proteins while minimizing protein complex disruption.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCompatible with Western blot analysis:\u003c\/strong\u003e Provides reliable whole-cell lysates for SDS-PAGE and immunoblotting workflows.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBroad sample compatibility:\u003c\/strong\u003e Suitable for adherent cells, suspension cells, and tissue protein extraction.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nAPPLICATIONS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eWestern \u0026amp; IP Cell Lysis Buffer is widely used as a native protein extraction reagent for molecular biology, cell biology, and biochemical research applications where preservation of protein characteristics is required.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eWestern Blot Protein Analysis:\u003c\/strong\u003e Preparation of whole-cell lysates for SDS-PAGE separation and Western blot detection of target proteins.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eImmunoprecipitation (IP):\u003c\/strong\u003e Provides compatible cell lysates for antibody-based protein enrichment while maintaining native antigen structures.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCo-Immunoprecipitation (Co-IP):\u003c\/strong\u003e Supports protein-protein interaction studies by preserving protein complexes during extraction.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eNative Protein Extraction:\u003c\/strong\u003e Suitable for experiments requiring functional proteins or preserved protein conformations.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eProtein Complex Analysis:\u003c\/strong\u003e Enables investigation of protein assemblies, signaling complexes, and molecular interactions.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCell Signaling Research:\u003c\/strong\u003e Supports analysis of signaling pathway proteins where antibody recognition and protein integrity are important.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eAntibody Binding Studies:\u003c\/strong\u003e Useful when antibodies recognize conformational epitopes that may be affected by denaturing extraction conditions.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBiochemical Research:\u003c\/strong\u003e Used for protein characterization, interaction analysis, and cellular protein studies.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPROTOCOL OVERVIEW\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eProtocol Overview\u003c\/h2\u003e\n\u003cp\u003eFor optimal protein extraction performance, sample preparation should be performed under cold conditions to minimize protein degradation and preserve native protein characteristics.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eAdd protease inhibitors such as PMSF immediately before use according to experimental requirements.\u003c\/li\u003e\n\u003cli\u003eAdd lysis buffer to prepared cell or tissue samples and incubate on ice for efficient protein extraction.\u003c\/li\u003e\n\u003cli\u003eCentrifuge lysates at 10,000–14,000 ×g for 3–5 minutes to remove insoluble debris.\u003c\/li\u003e\n\u003cli\u003eCollect the clarified supernatant for Western blot, IP, Co-IP, or other protein analysis workflows.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eBecause this buffer contains detergent components, BCA protein assays are recommended for protein concentration measurement instead of Bradford assays.\u003c\/p\u003e\n\u003ch2\u003eOrdering Information\u003c\/h2\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"8\" border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eRP0131\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eWestern \u0026amp; IP Cell Lysis Buffer\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFAQ\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is Western \u0026amp; IP Cell Lysis Buffer used for?\u003c\/summary\u003e\n\u003cp\u003eWestern \u0026amp; IP Cell Lysis Buffer is a non-denaturing cell lysis buffer designed for protein extraction before Western blot, immunoprecipitation (IP), and co-immunoprecipitation (Co-IP) experiments. It helps maintain native protein structures and protein interactions during sample preparation.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the difference between IP lysis buffer and RIPA buffer?\u003c\/summary\u003e\n\u003cp\u003eIP lysis buffer is optimized for maintaining protein complexes and native antigen structures, while RIPA buffer generally provides stronger protein solubilization. For protein-protein interaction studies and Co-IP experiments, a mild non-denaturing lysis buffer is usually preferred.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan this cell lysis buffer be used for Western blot?\u003c\/summary\u003e\n\u003cp\u003eYes. Western \u0026amp; IP Cell Lysis Buffer can be used for Western blot sample preparation. The extracted whole-cell lysates are compatible with SDS-PAGE and antibody-based protein detection workflows.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhy is a non-denaturing lysis buffer important for immunoprecipitation?\u003c\/summary\u003e\n\u003cp\u003eNon-denaturing lysis conditions help preserve protein conformation and protein complexes, allowing antibodies to recognize native epitopes and enabling more reliable immunoprecipitation results.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan this buffer be used for Co-IP protein interaction studies?\u003c\/summary\u003e\n\u003cp\u003eYes. This Co-IP compatible lysis buffer is designed to preserve protein-protein interactions and is suitable for studying protein complexes and cellular signaling interactions.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhich protein assay method is recommended after extraction?\u003c\/summary\u003e\n\u003cp\u003eBCA protein assays are recommended because detergent-containing lysis buffers may interfere with Bradford protein quantification methods.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPRODUCT SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c!-- =========================\nFAQ SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"AccuRef Scientific","offers":[{"title":"100mL","offer_id":52776381644981,"sku":"RP0131","price":61.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/6_74344d42-4293-45a5-ba6f-168adc29183f.jpg?v=1783495077","url":"https:\/\/biofargo.com\/products\/western-ip-cell-lysis-buffer","provider":"Biofargo","version":"1.0","type":"link"}