Description

YamayBio Western Blot Rapid Stripping buffers are essential tools for removing primary and secondary antibodies from membranes, enabling reprobing, saving time and costs when sample quantities are limited . YamayBio Western Blot Rapid Stripping Buffer is specifically formulated to gently and efficiently strip target proteins from PVDF and NC membranes, minimizing damage to the membrane. Its rapid action and odorless, environmentally friendly formulation ensure a safe and effective process.

Features

Efficient:

Better than traditional stripping buffer.
Mild and General:

Do not strip target.

The mild formula of Yamay Rapid Stripping buffer minimizes damage to antigens and membranes.

Western Blot Rapid Stripping Buffer -mild-and-general-type-Yamaybio

Fig 1. Hela cells lysate (2.5μg, 1.25μg, 0.62μg, 0.31μg of total protein) were probed with GAPDH. Following the initial detection,each blot was cut into two strips, one part of the blot was stripped in BM3154, the other was stripped in Thermo Scientific Restore Western Blot Stripping buffer (Part No.21059), for a long time stripping (6 hours). After the stripping procedure, the membrane strips were washed in 1× PBS Tween-20 buffer and incubated with the substrate and imaged.The membrane strips were reblocked and reprobed for GAPDH, the Western blot procedure repeated as described above.

The rapid stripping buffer is compatible with both PVDF and NC membranes.

Western Blot Rapid Stripping Buffer -mild-and-general-type-Yamaybio

Fig 2. A431 cells lysate (2.5μg, 1.25μg, 0.62μg, 0.31μg of total protein) were separated by SDS-PAGE and transferred to PVDF membranes or nitrocellulose (NC) membranes .The membranes were blocked by Protein-free Blocking Buffer (Part No.BM3152) and probed with an anti-GAPDH antibody. Following the initial detection, each blot was cut into two strips. One strip was stripped in Yamay rapid stripping buffer BM3154, while the other was stripped in Thermo Scientific Restore Western Blot Stripping buffer (Part No.21059) for 15 minutes at room temperature, according to the manufacturer's instructions. After the stripping procedure, the membrane strips were washed in 1× PBS Tween-20 buffer and incubated with the substrate and imaged. The membrane strips were reblocked and reprobed for an anti-HSP60 antibody.

Specifications

Product Type	Western Blot Stripping Buffer
Form	Liquid
Application	Western Blot
Membrane Compatibility	Nitrocellulose, PVDF
Quantity	100 mL / 500 mL

Storage

Transported at room temperature and stored at 4°C. The shelf life is 12 months.

Documents

BM3154-100 COA

BM3154-500 COA

SDS

User Guide

Flyer of Product_ Western Blot Rapid Stripping Buffer (mild and general type)

Western Blot Rapid Stripping Buffer-mild-and-general-type-Yamaybio