{"product_id":"western-blot-incubation-washing-box","title":"Western Blot Incubation \u0026 Washing Box","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eThe \u003cstrong\u003eWestern Blot Incubation \u0026amp; Washing Box \u003c\/strong\u003eis a laboratory-grade container designed for \u003cstrong\u003eprotein membrane incubation, antibody binding, washing, and gel staining workflows\u003c\/strong\u003e in Western blot experiments.\u003c\/p\u003e\n\u003cp\u003eIt provides a stable, contamination-free environment for \u003cstrong\u003eantibody incubation, membrane handling, and signal development steps\u003c\/strong\u003e, ensuring consistent experimental results and improved reproducibility.\u003c\/p\u003e\n\u003cp\u003eUnlike standard plastic trays, this blotting box features a \u003cstrong\u003enon-binding internal surface\u003c\/strong\u003e that minimizes antibody adsorption and protein loss, helping maintain \u003cstrong\u003estrong signal intensity and low background noise\u003c\/strong\u003e.\u003c\/p\u003e\n\u003cp\u003eA \u003cstrong\u003elight-protective opaque design\u003c\/strong\u003e is available for fluorescence and chemiluminescence-based detection workflows, reducing signal degradation caused by ambient light exposure.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eMaterial: Laboratory-grade chemical resistant polymer\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSurface treatment: Non-binding \/ low protein adsorption\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eColor: Transparent \/ Black (light-protective version)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eCompatibility: Western blot membranes (PVDF \/ NC)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eApplication temperature: Room temperature use\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eReusability: Reusable after cleaning and sterilization (if applicable)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSizes: Multiple formats available for gel and membrane workflows\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eFeatures\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eOptimized for Western blot workflow\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eSuitable for antibody incubation, membrane washing, blocking, and staining\/destaining steps\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eNon-binding internal surface\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eReduces antibody and protein adsorption to improve signal quality and reproducibility\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eLight-protective design (optional black version)\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIdeal for fluorescent and chemiluminescent detection systems\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eSmooth inner surface\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eEnsures even reagent distribution and protects delicate membranes from damage\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eChemical-resistant material\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eCompatible with common Western blot buffers and staining solutions (TBST, PBS, methanol-based reagents)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eMultiple size options\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eSupports different membrane sizes and gel formats for flexible experimental use\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eWestern blot antibody incubation\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eMembrane washing (TBST \/ PBST steps)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eProtein gel staining and destaining\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eChemiluminescent and fluorescence detection workflows\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eImmunoblot signal development and optimization\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eProtein expression and biomarker analysis\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eWhy This Box Matters in Western Blot Workflow\u003c\/h2\u003e\n\u003cp\u003eWestern blot performance is not only determined by antibodies and detection systems, but also by\u003cstrong\u003e every incubation and washing step\u003c\/strong\u003e.\u003c\/p\u003e\n\u003cp\u003eThis incubation box helps solve common experimental problems:\u003c\/p\u003e\n\u003cp\u003eHigh background caused by uneven washing\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eWeak signal due to antibody adsorption on plastic surfaces\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSignal loss in fluorescence-based detection due to light exposure\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eMembrane damage during handling and transfer\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eBy optimizing the \u003cstrong\u003ephysical environment of incubation\u003c\/strong\u003e, this box improves overall experimental consistency and data reliability.\u003c\/p\u003e\n\n\u003ch2\u003eTechnical Advantages vs Standard Containers\u003c\/h2\u003e\n\u003cdiv style=\"overflow-x: auto; border: 1px solid #999; border-radius: 4px;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; min-width: 900px; font-size: 14px; font-family: 'Helvetica Neue', Helvetica, Arial, sans-serif; height: 171px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"background: #0056a6; color: #ffffff; font-weight: bold;\"\u003e\n\u003ctd style=\"border: 1px solid #999999; padding: 10px; width: 33.3333%; text-align: center; height: 19px;\"\u003eFeature\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #999999; padding: 10px; width: 33.3333%; text-align: center; height: 19px;\"\u003eStandard Plastic Tray\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #999999; padding: 10px; width: 33.3333%; text-align: center;\"\u003eWestern Blot Incubation Box\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"background: #f7f9fb; font-weight: bold; border: 1px solid #999999; padding: 10px; width: 33.3333%; text-align: center; height: 19px;\"\u003eSurface binding\u003c\/td\u003e\n\u003ctd style=\"background: #f7f9fb; font-weight: bold; border: 1px solid #999999; padding: 10px; width: 33.3333%; text-align: center; height: 19px;\"\u003eHigh protein loss\u003c\/td\u003e\n\u003ctd style=\"background: #f7f9fb; font-weight: bold; border: 1px solid #999999; padding: 10px; width: 33.3333%; text-align: center;\"\u003eLow-binding surface\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"background: #f7f9fb; font-weight: bold; border: 1px solid #999999; padding: 10px; width: 33.3333%; text-align: center; height: 19px;\"\u003eLight protection\u003c\/td\u003e\n\u003ctd style=\"background: #f7f9fb; font-weight: bold; border: 1px solid #999999; padding: 10px; width: 33.3333%; text-align: center; height: 19px;\"\u003eNone\u003c\/td\u003e\n\u003ctd style=\"background: #f7f9fb; font-weight: bold; border: 1px solid #999999; padding: 10px; width: 33.3333%; text-align: center;\"\u003eOpaque \/ light-blocking\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"background: #f7f9fb; font-weight: bold; border: 1px solid #999999; padding: 10px; width: 33.3333%; text-align: center; height: 19px;\"\u003eWashing uniformity\u003c\/td\u003e\n\u003ctd style=\"background: #f7f9fb; font-weight: bold; border: 1px solid #999999; padding: 10px; width: 33.3333%; text-align: center; height: 19px;\"\u003eInconsistent\u003c\/td\u003e\n\u003ctd style=\"background: #f7f9fb; font-weight: bold; border: 1px solid #999999; padding: 10px; width: 33.3333%; text-align: center;\"\u003eEven reagent distribution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"background: #f7f9fb; font-weight: bold; border: 1px solid #999999; padding: 10px; width: 33.3333%; text-align: center;\"\u003eMembrane safety\u003c\/td\u003e\n\u003ctd style=\"background: #f7f9fb; font-weight: bold; border: 1px solid #999999; padding: 10px; width: 33.3333%; text-align: center;\"\u003eRisk of damage\u003c\/td\u003e\n\u003ctd style=\"background: #f7f9fb; font-weight: bold; border: 1px solid #999999; padding: 10px; width: 33.3333%; text-align: center;\"\u003eSmooth protective surface\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"background: #f7f9fb; font-weight: bold; border: 1px solid #999999; padding: 10px; width: 33.3333%; text-align: center; height: 19px;\"\u003eWorkflow compatibility\u003c\/td\u003e\n\u003ctd style=\"background: #f7f9fb; font-weight: bold; border: 1px solid #999999; padding: 10px; width: 33.3333%; text-align: center; height: 19px;\"\u003eGeneral use\u003c\/td\u003e\n\u003ctd style=\"background: #f7f9fb; font-weight: bold; border: 1px solid #999999; padding: 10px; width: 33.3333%; text-align: center;\"\u003eWestern blot optimized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eStore at room temperature in a clean, dry environment\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAvoid strong organic solvents unless compatibility is confirmed\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eClean with mild detergent or laboratory-grade cleaning solutions\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eDo not expose to prolonged UV or harsh chemical sterilization unless validated\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003cdetails\u003e\n\u003csummary\u003e\u003cstrong\u003eWhat is a Western blot incubation box used for?\u003c\/strong\u003e\u003c\/summary\u003e\n\u003cdiv style=\"padding-top: 10px;\"\u003eIt is used for antibody incubation, membrane washing, and staining steps during Western blot experiments, providing a stable and contamination-free environment.\u003c\/div\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003e\u003cstrong\u003eWhy is a non-binding surface important?\u003c\/strong\u003e\u003c\/summary\u003e\n\u003cdiv style=\"padding-top: 10px;\"\u003eNon-binding surfaces reduce antibody adsorption to plastic walls, helping maintain higher signal intensity and lower background noise.\u003c\/div\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003e\u003cstrong\u003eCan this box be used for fluorescence detection workflows?\u003c\/strong\u003e\u003c\/summary\u003e\n\u003cdiv style=\"padding-top: 10px;\"\u003eYes. The opaque light-protective version is designed for fluorescence and chemiluminescence applications to minimize signal degradation.\u003c\/div\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003e\u003cstrong\u003eIs this suitable for PVDF and nitrocellulose membranes?\u003c\/strong\u003e\u003c\/summary\u003e\n\u003cdiv style=\"padding-top: 10px;\"\u003eYes. It is compatible with both PVDF and nitrocellulose membranes commonly used in Western blot experiments.\u003c\/div\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003e\u003cstrong\u003eCan it be reused?\u003c\/strong\u003e\u003c\/summary\u003e\n\u003cdiv style=\"padding-top: 10px;\"\u003eYes, it is designed for repeated laboratory use when properly cleaned and maintained.\u003c\/div\u003e\n\u003c\/details\u003e","brand":"azurebiosystems","offers":[{"title":"Black \/ 7 * 5 cm \/ 1 Pcs","offer_id":52706560671925,"sku":"AC2120","price":19.0,"currency_code":"USD","in_stock":true},{"title":"Black \/ 9 * 6 cm \/ 1 Pcs","offer_id":52706560639157,"sku":"AC2121","price":19.0,"currency_code":"USD","in_stock":true},{"title":"Black \/ 11 * 9 cm \/ 1 Pcs","offer_id":52706560606389,"sku":"AC2122","price":19.0,"currency_code":"USD","in_stock":true},{"title":"Black \/ 15.5 * 10.5 cm \/ 1 Pcs","offer_id":52706560573621,"sku":"AC2123","price":19.0,"currency_code":"USD","in_stock":true},{"title":"Clear \/ 7 * 5 cm \/ 1 Pcs","offer_id":52706560802997,"sku":"AC2150","price":19.0,"currency_code":"USD","in_stock":true},{"title":"Clear \/ 9 * 6 cm \/ 1 Pcs","offer_id":52706560770229,"sku":"AC2151","price":19.0,"currency_code":"USD","in_stock":true},{"title":"Clear \/ 11 * 9 cm \/ 1 Pcs","offer_id":52706560737461,"sku":"AC2152","price":19.0,"currency_code":"USD","in_stock":true},{"title":"Clear \/ 15.5 * 10.5 cm \/ 1 Pcs","offer_id":52706560704693,"sku":"AC2153","price":19.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/2_377c582e-d031-4639-b1b4-9bacf3f2b6f8.jpg?v=1781514357","url":"https:\/\/biofargo.com\/products\/western-blot-incubation-washing-box","provider":"Biofargo","version":"1.0","type":"link"}