Description

SHENTEK® Virus DNA & RNA Extraction Kit is suitable for extraction of viral DNA and RNA from up to 107 cells, and also for nucleic acid extraction of harvested virus bulk (liquid). The kit is compatible with either manual sample preparation or automated extraction with SHENTEK® rHCDpurify system. Depending on subcellular location of viral replication, the extraction methods for cells can be grouped into two categories:

Method 1: For viruses replicated in host nucleus including retroviruses, such as most DNA viruses (rcAAV/rAAV) as well as replication competent lentivirus (RCL) and relication competent retrovirus (RCR), additional nuclease digestion after cell lysis can reduce the risk of false negative. Method 1 is also recommended for detection of samples contaminated with unknown viruses.

Method 2: For viruses replicated in host cytoplasm, for example most RNA viruses, cell lysis followed by centrifugation and extraction of the supernatant is sufficient for detection purposes.

Overview

Components	Shipping Condition	Unit Size	Detection Method
Wash buffer A	room temperature	50 Extractions	Magnetic particle separation
Binding solution
Proteinase K Buffer
Pretreatment buffer	2-8℃
Magnetic particles
5M NaCl
Precipitation solution I	-20℃
RNase-Free H2O
Proteinase K

Specification

Recovery	at least 90% to fg/μL
Repeatability	CV no more than 15%

Documents

User Guide

Virus DNA & RNA Extraction Kit SHENTEK®