Description

This vero elisa kit is based on the solid-phase enzyme-linked immunosorbent assay (ELISA) with a double-antibody sandwich technique to detect residual host cell proteins (HCPs) from vero cells. A sheep polyclonal antibody specific to vero HCPs was employed in the assay to capture any remaining HCPs in the sample. The antibody coverage is assessed by the current mainstream method. Both the Calibration standards (or test sample) and the HRP (Horseradish Peroxidase) labeled anti-Vero HCP antibody were simultaneously added to the microtiter plate, which coated with the affinity purified capture antibody and followed by incubation and washing. Then TMB (3,3',5,5'-tetramethylbenzidine) substrate was added into reaction, HRP catalyzed the oxidation of TMB by H₂O₂ to produce a blue product (maximum absorption peak at 655 nm). Then the stop solution was added to terminate the enzymatic reaction, resulting in a yellow colored product (maximum absorption peak at 450nm). The absorbance values at 450 nm wavelength were positively correlated with the HCPs concentration in the Calibration standard and the sample. The concentration of HCPs in the sample can be calculated using the dose-response curve.

Components	Shipping Condition	Unit Size	Detection Method
Vero HCP Calibration Standard	2-8℃	96 Tests	Sandwich ELISA
Anti-Vero HCP Microtiter Strips
Reconstitution Solution
Diluent
Wash Buffer Concentrate (10×)
Anti-Vero: HRP (100×)
TMB Substrate
Stop Solution
Sealing Film

Specifications

Linearity & Range	3-243 ng/mL, R2>0.990
Accuracy	Recovery Rate= 93.4%-108.1%
LLOQ	3 ng/mL
LOD	1 ng/mL
Precision	2.8%-5.0% (CV≤20%)
Antibody Coverage (IMBS-2D)	65.1%-85.1%
Antibody Coverage (IMBS-LC/MS)	76.90%
Specificity	No cross-reactivity with E.coli, P.pastoris and Sf9 cell.
Robustness	Incubation speed: 500 rpm - 600 rpm

Related Products

SHENTEK^® E.coli (Protein Expression Strains) HCP ELISA Kit
SHENTEK^® E.coli (Protein Expression Strains) HCP ELISA Kit (One-step ELISA)
SHENTEK^® Anti-E.coli (Protein Expression Strains) HCP Antibody
SHENTEK^® E.coli (K-12 & Alkaline Lysis) HCP ELISA Kit
SHENTEK^® Anti-E.coli (K-12 & Alkaline Lysis) HCP Antibody
SHENTEK^® CHO HCP ELISA Kit (One-step ELISA)
SHENTEK^® CHO-K1 HCP ELISA Kit (One-step ELISA)
SHENTEK^® MDCK HCP ELISA Kit (One-step ELISA)
SHENTEK^® Anti-MDCK HCP Antibody
SHENTEK^® Vero HCP ELISA Kit (One-step ELISA)
SHENTEK^® Anti-Vero HCP Antibody
SHENTEK^® Sf9 HCP ELISA Kit (One-step ELISA)
SHENTEK^® Anti-Sf9 HCP Antibody
SHENTEK^® HEK293 HCP ELISA Kit (One-step ELISA)
SHENTEK^® Anti-HEK293 HCP Antibody
SHENTEK^® P.pastoris HCP ELISA Kit (One-step ELISA)

Documents

User Guide

Vero HCP ELISA Kit SHENTEK®