Description

Variola Virus(VARV) Probe qPCR Kit is a PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified DNA is directly amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification. The resulting signals may be used for comparative analysis of target nucleic acids.

Note: Supplied without internal control. Internal control version can be customized based on your assay requirements.
This product was previously listed under catalog number 15-11100 and is now updated to BF-95368753 for improved product management.

Key Features

Streamlined PCR workflow to reduce handling steps and minimize contamination risk

Demonstrates high sensitivity under optimized laboratory conditions.
High specificity based on primer-probe design.
Includes a non-infectious plasmid-based positive control for assay verification.
Compatible with commonly used DNA purification methods.
Suitable for research applications including assay development and method evaluation.
Broad dynamic range under laboratory conditions.
For research use only. Not intended for diagnostic or therapeutic use.

Kit Components

Component Name	Description	Size	Vial	Lid Color
2×Probe qPCR Master Mix	Contains optimized buffer and enzyme mix	500 μL	0.5 mL Vial	Blue
Primer-Probe Powder	Lyophilized target-specific primer and probe set	50 T	1.5 mL Vial	Brown
Positive Control (1E7 copies/μL)	Non-infectious plasmid-based control	0.5 mL	0.5 mL Brown Vial	Yellow
Nuclease-free water / Dilution Buffer	For reaction setup	1 mL	1.5 mL Vial	Green
Manual	Instruction booklet	1 Set	NA	NA

Application Areas

Molecular biology research: qPCR-based detection and quantification of target nucleic acids.
Assay development: Suitable for method development, optimization, and analytical performance evaluation.
Industry applications (research use): Widely used in biotechnology, pharmaceutical, academic, and agricultural research laboratories.
For Research Use Only (RUO). Not for use in diagnostic procedures.

Storage & Shipping

Store at - 20 °C
The product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.

FAQ

Q: Are Biofargo PCR and qPCR Kits compatible with my thermal cycler or real-time PCR instrument?

A: Yes. Biofargo PCR Kits and probe-based qPCR Kits are designed for broad compatibility with mainstream instruments.

PCR Kits can be used with standard thermal cyclers from leading brands.

Probe-based qPCR Kits are fully compatible with major real-time PCR platforms, including ABI, Bio-Rad, Roche, QuantStudio, Qiagen Rotor-Gene Q (5-plex), and Genesig Q16.

Our technical team is available to assist with instrument setup and run optimization to ensure the best results.

Documents

Variola Virus(VARV) Probe qPCR Kit User Manual

MSDS