Key Features

1. Safer than EB. Shown by Ames test and other tests to be non-mutagentic and non-cytotoxic.

2. Stable at room temperature.

3. Compatible with standard blue LED and UV transilluminators.

4. Use with blue light reduces mutagenesis for gel excision and subsequent cloning.

Description

Ultra Nucleic Acid Gel Stain is a ultra sensitive green fluorescent stain for detecting nucleic acids in agarose and polyacrylamide gels. This stain can detect as little as 50 pg of dsDNA. Ultra is compatible with a standard 300 nm transilluminator, a 254 nm transilluminator, a blue-light transilluminator, or a gel reader equipped with visible light excitation such as a 488 nm laser-based gel scanner. Ultra Nucleic Acid Gel Stain, 10,000× is a concentrated Ultra solution that can be diluted 10,000 times for use in precast gel staining or 5,000 times for use in post gel staining according to the procedures described below. One vial of 10,000× solution can be used to prepare at least 100 precast minigels or post-stain at least 100 minigels.

Gel staining with Ultra is compatible with downstream applications such as gel extraction and cloning. Ultra is efficiently removed from DNA by phenol/chloroform extraction and ethanol precipitation.

Component

1. Ultra Nucleic Acid Gel Stain (10,000× in DMSO): 500 μl

Storage

Store at 2-25°C and protect from light.

Documents

Manual