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When cells undergo apoptosis, they activate endonuclease enzymes that cut the genomic DNA between nucleosomes. After DNA was extracted during apoptosis, a 180-200 bp DNA ladder could be found.
TUNEL (TdT mediated dUTP Nick End Labeling) Apoptosis detection kit (Alexa Fluor 640) can be used to detect nuclear DNA fragmentation in the late apoptotic process of tissue cells. The principle is that under the action of Terminal Deoxynucleotidyl Transferase (TdT), Alexa Fluor 640-12-DUTP is incorporated into the 3′ -hydroxyl (3′-OH) Terminal exposed during genomic DNA break. Thus, it can be detected by fluorescence microscopy or flow cytometry. Alexa Fluor 640 is a red fluorescent dye with high stability and strong brightness.
This kit has a wide range of applications and can be used to detect apoptosis in frozen or paraffin sections, as well as in cultured adherent or suspended cells.
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