Description

VeZol Reagent is a high-performance total RNA extraction reagent designed for rapid isolation of high-quality RNA from cultured cells, animal tissues, and plant tissues. In many molecular biology workflows, inefficient sample disruption, RNase contamination, and inconsistent RNA recovery can compromise downstream experiments. VeZol Reagent provides a reliable solution through a classic phenol and guanidine isothiocyanate-based RNA extraction method, enabling efficient cell lysis, RNase inhibition, and high-quality total RNA recovery.

VeZol Reagent is a monophasic RNA extraction reagent containing phenol, guanidine isothiocyanate, and optimized proprietary components for enhanced sample disruption and RNA protection. After sample lysis and chloroform extraction, the lysate separates into an upper aqueous phase containing RNA, an interphase containing genomic DNA, and a lower organic phase containing proteins and other cellular components.

Following isopropanol precipitation and ethanol washing, the extracted total RNA can be directly used for a wide range of molecular biology applications, including RT-PCR, RT-qPCR, Northern Blot, Dot Blot, in vitro translation, and RNA sequencing (RNA-seq).

With broad sample compatibility and a proven organic extraction workflow, VeZol Reagent serves as a reliable TRIzol alternative for laboratories requiring consistent RNA isolation performance from diverse biological samples.

For research use only. Not for use in diagnostic procedures.

Specification

Product Name	VeZol Reagent
Product Type	Total RNA Extraction Reagent
Extraction Method	Phenol and Guanidine Isothiocyanate-Based Organic Extraction
Sample Types	Cultured Cells, Animal Tissues, Plant Tissues
Components	VeZol Reagent
Available Sizes	100 mL / 200 mL
Storage	2–8°C, Protect from Light
Transportation	Room Temperature Transport
Downstream Applications	RT-PCR, RT-qPCR, Northern Blot, Dot Blot, In Vitro Translation, RNA Sequencing
Recommended Sample Amount	Cells: 1 × 10⁵ – 1 × 10⁷ cells Animal/Plant Tissue: 50–100 mg per 1 mL reagent
Research Use	Molecular Biology Research Only

Features

High-Quality Total RNA Isolation: Enables efficient recovery of purified total RNA suitable for downstream molecular biology applications including RT-PCR, RT-qPCR, and RNA sequencing.
Phenol Guanidine Isothiocyanate-Based Extraction: Utilizes a classical organic extraction workflow for reliable RNA separation and high recovery efficiency.
Strong Sample Lysis Capability: Provides effective disruption of cultured cells, animal tissues, and plant tissues to improve RNA release.
Effective RNase Inhibition: The optimized formulation helps protect RNA integrity by rapidly denaturing proteins and inhibiting RNase activity during extraction.
Broad Sample Compatibility: Suitable for RNA extraction from various biological samples, including adherent cells, suspension cells, animal tissues, and plant materials.
Reliable Phase Separation: Forms distinct aqueous, interphase, and organic phases after chloroform extraction for efficient RNA recovery.
Compatible with Multiple Downstream Workflows: Extracted RNA can be directly applied to gene expression analysis, transcriptome research, and molecular biology assays.

Applications

RNA Extraction from Cultured Cells: Used for total RNA isolation from adherent and suspension cell cultures for gene expression analysis and molecular studies.
Animal Tissue RNA Extraction: Suitable for extracting RNA from animal tissues requiring efficient homogenization and RNase protection.
Plant Tissue RNA Isolation: Supports RNA extraction from plant samples after effective tissue disruption and grinding procedures.
RT-PCR and RT-qPCR Analysis: Provides high-quality RNA templates for reverse transcription and quantitative gene expression experiments.
RNA Sequencing Sample Preparation: Suitable for preparing total RNA samples used in transcriptome profiling and high-throughput sequencing workflows.
Gene Expression Research: Enables investigation of transcriptional regulation, RNA abundance changes, and biological pathway studies.
Northern Blot and Dot Blot Applications: Supports RNA detection and analysis based on traditional hybridization methods.
Molecular Biology Research: Applicable to general RNA purification workflows in academic, biotechnology, and pharmaceutical research laboratories.

Documents

Manual

Frequently Asked Questions

What is VeZol Reagent used for in RNA extraction?

VeZol Reagent is a phenol and guanidine isothiocyanate-based total RNA extraction reagent designed for isolating high-quality RNA from cultured cells, animal tissues, and plant tissues. It is commonly used for molecular biology workflows including RT-PCR, RT-qPCR, RNA sequencing, and gene expression analysis.

How does VeZol Reagent protect RNA from degradation?

VeZol Reagent contains guanidine isothiocyanate, which rapidly denatures proteins and inhibits RNase activity during sample lysis. This helps preserve RNA integrity and improves the reliability of total RNA isolation for downstream applications.

Can VeZol Reagent be used for RNA extraction from cells and tissues?

Yes. VeZol Reagent is suitable for RNA extraction from a wide range of biological samples, including adherent cells, suspension cells, animal tissues, and plant tissues. The reagent provides strong lysis capability for efficient sample disruption and RNA recovery.

What is the difference between VeZol Reagent and column-based RNA extraction kits?

VeZol Reagent uses a classical phenol-chloroform organic extraction workflow, which can provide high RNA recovery from diverse samples. Compared with column-based RNA extraction kits, organic extraction methods are widely used for laboratories requiring flexible sample compatibility and high total RNA yield.

Is VeZol Reagent compatible with RT-qPCR experiments?

Yes. RNA extracted using VeZol Reagent can be used as a template for reverse transcription and quantitative PCR applications when appropriate RNA quality and purity requirements are met.

Can VeZol Reagent be used for RNA sequencing workflows?

Yes. VeZol Reagent can generate total RNA suitable for RNA sequencing sample preparation and transcriptome research when extracted RNA meets the quality requirements of the sequencing workflow.

What sample amount can be processed with VeZol Reagent?

With 1 mL of VeZol Reagent, recommended sample amounts include approximately 1 × 10⁵–1 × 10⁷ adherent cells, 5 × 10⁶–1 × 10⁷ suspension cells, or 50–100 mg animal or plant tissue.

How should VeZol Reagent be stored?

VeZol Reagent should be stored at 2–8°C and protected from light. During transportation, the reagent can be shipped at room temperature according to product guidelines.

Is VeZol Reagent a replacement for TRIzol Reagent?

VeZol Reagent follows the same phenol and guanidine isothiocyanate-based RNA extraction principle as commonly used TRIzol-style reagents. It can serve as an alternative solution for researchers looking for reliable total RNA extraction performance from biological samples.

What precautions should be taken during RNA extraction?

RNA extraction should be performed under RNase-free conditions. Use RNase-free consumables, wear appropriate protective equipment, avoid contamination, and follow proper handling procedures when working with phenol-containing reagents.

For laboratory research or industrial use only.

Trizol Reagent