Synonym(s): TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride

Description

Tris-HCl (Tris(hydroxymethyl)aminomethane hydrochloride) is a high-purity biochemical-grade buffering reagent supplied as white crystalline powder. Its molecular formula is C₄H₁₁NO₃·HCl, with a molecular weight of 157.60 and CAS number 1185‑53‑1. Tris-HCl dissolves readily in water to form a clear solution, providing stable pH control in biochemical, molecular biology, and cell biology experiments.

Specifications

Item	Value
Chemical Name	Tris(Hydroxymethyl)Aminomethane Hydrochloride
Appearance	White Crystal
CAS No.	1185-53-1
Molecular Formula	C4H11NO3·HCl
Molecular Weight	157.60
Grade	Biochemical Grade
Purity	≥99.0%
Melting Point	150.0–153.0℃
Loss on Drying	≤0.4%
Residue on Ignition	≤0.1%
Water Solubility	Clear
Heavy Metals	≤5 ppm
Iron	≤1 ppm
Moisture	≤0.5%
pH (1.0mol/L)	3.7–4.7
PKa (20℃)	8.0–8.4
UV Absorbance (260nm, 1mmol/L, 1cm)	≤0.02
UV Absorbance (280nm, 1mmol/L, 1cm)	≤0.02

Specifications

High Purity: ≥99.0% biochemical grade ensures minimal contaminants for reliable experiments.
Stable pH Buffering: pH range of 3.7–4.7 (1.0 mol/L solution), pKa 8.0–8.4 at 20°C.
Excellent Solubility: Produces clear solutions suitable for sensitive assays.
Low Metal Content: Heavy metals ≤5 ppm, iron ≤1 ppm, suitable for molecular biology applications.
Low UV Absorbance: A260 and A280 ≤0.02, ideal for protein and nucleic acid assays.

Applications

Buffer preparation for protein purification, enzyme reactions, and molecular biology workflows.
Electrophoresis buffers for DNA, RNA, and protein analysis.
Cell culture media requiring stable pH for growth and metabolic activity.
General laboratory buffer systems for consistent and reproducible experimental results.

Safety Disclaimer

This product is intended for laboratory research use only. Not for drug, human, or veterinary use. Review the Safety Data Sheet (SDS) provided by BioFargo for detailed handling, storage, hazard, and disposal information. Use appropriate personal protective equipment (PPE) including gloves, lab coat, and eye protection when handling. Dispose of waste and residual buffer in accordance with local, state, and federal regulations. Ensure proper ventilation if preparing large volumes or heating solutions.

Documents

MSDS

Tris-HCl 99% Biochemical Grade