Description

During routine SDS-PAGE protein electrophoresis workflows, preparing running buffer manually can introduce variability caused by repeated weighing, reagent handling, and preparation errors. Tris-Glycine-SDS Electrophoresis Buffer Instant Powder provides a convenient solution for researchers who need a consistent SDS-PAGE running buffer preparation workflow with reduced preparation steps.

Tris-Glycine-SDS Electrophoresis Buffer Instant Powder is a pre-measured powder formulation designed for preparation of Tris-Glycine-SDS running buffer used in denaturing polyacrylamide gel electrophoresis. Each package can be dissolved and brought to a final volume of 1 L to prepare SDS-PAGE electrophoresis buffer for protein separation applications.

This SDS-PAGE running buffer contains SDS and is suitable for Tris-HCl based polyacrylamide gel electrophoresis systems. It is commonly used for denaturing protein electrophoresis workflows, including protein separation prior to downstream analysis such as Western blotting. This product is not suitable for polyacrylamide gel systems using alternative buffer systems such as HEPES.

The instant powder format provides convenient room temperature storage and efficient laboratory preparation compared with manually prepared electrophoresis buffers.

Specifications

Product Name	Tris-Glycine-SDS Electrophoresis Buffer Instant Powder
SKU	AP0541
Product Type	SDS-PAGE Running Buffer Powder
Format	Instant Powder
Specification	10 × 1 L
Applications	SDS-PAGE, protein electrophoresis, Western blot workflows
Compatible Gel System	Tris-HCl based polyacrylamide gels
Storage	Room Temperature
Shelf Life	2 years

Features

Convenient powder format: Pre-measured instant powder simplifies SDS-PAGE running buffer preparation and reduces manual reagent handling.
Designed for SDS-PAGE protein electrophoresis: Contains SDS and supports denaturing protein separation using Tris-Glycine buffer systems.
Consistent buffer preparation: Each package is prepared to a final volume of 1 L for convenient laboratory use.
Room temperature storage: Powder format enables convenient storage with a 2-year shelf life under recommended conditions.
Compatible with downstream protein analysis: Suitable for protein separation workflows before applications such as Western blot analysis.
Reusable running buffer option: Prepared electrophoresis buffer may be recovered and reused as an outer chamber running buffer for limited cycles.

Applications

Tris-Glycine-SDS Electrophoresis Buffer Instant Powder is designed for protein electrophoresis workflows requiring reliable SDS-PAGE running buffer preparation.

SDS-PAGE Protein Electrophoresis: Used as a running buffer for denaturing polyacrylamide gel electrophoresis to separate proteins according to molecular characteristics.
Western Blot Sample Preparation: Supports protein separation workflows before transfer and antibody-based protein detection.
Protein Molecular Analysis: Provides electrophoresis conditions for routine protein characterization and comparison studies.
Recombinant Protein Research: Suitable for analyzing protein expression profiles during molecular biology research workflows.
Cell Biology Research: Used with protein lysates prepared from biological samples for SDS-PAGE analysis.
Biochemical Research: Supports common protein separation workflows requiring Tris-Glycine-SDS electrophoresis systems.

Protocol Overview

One package of Tris-Glycine-SDS Electrophoresis Buffer Instant Powder is used to prepare 1 L SDS-PAGE running buffer.

Add one package of powder into a clean container.
Add approximately 900 mL distilled water and dissolve completely.
Adjust the final volume to 1 L with distilled water.
Mix thoroughly before use in SDS-PAGE electrophoresis.

Unused prepared buffer can typically be stored at room temperature for 2–3 days or at 4°C for 1–2 weeks. For optimal electrophoresis performance, freshly prepared running buffer is recommended.

Ordering Information

SKU	Product	Size
AP0541	Tris-Glycine-SDS Electrophoresis Buffer Instant Powder	10 × 1 L

FAQ

What is Tris-Glycine-SDS Electrophoresis Buffer used for?

Tris-Glycine-SDS Electrophoresis Buffer is a running buffer used for SDS-PAGE protein electrophoresis. It supports denaturing protein separation in Tris-HCl based polyacrylamide gel systems.

What is the difference between Tris-Glycine-SDS buffer and native PAGE buffer?

Tris-Glycine-SDS running buffer contains SDS and is designed for denaturing protein electrophoresis. Native PAGE workflows typically require different buffer systems without SDS.

Can this SDS-PAGE running buffer be used for Western blot workflows?

Yes. This Tris-Glycine-SDS running buffer can be used during protein separation before downstream Western blot analysis.

How do I prepare the instant powder electrophoresis buffer?

Dissolve one package of powder in distilled water, initially using approximately 900 mL water, then adjust the final volume to 1 L before use.

Can prepared running buffer be reused?

Prepared electrophoresis buffer may be recovered as an outer chamber running buffer and reused 1–2 times. For optimal SDS-PAGE performance, fresh running buffer is recommended.

Is this buffer compatible with HEPES-based PAGE systems?

No. This product is designed for Tris-HCl based polyacrylamide gel electrophoresis systems and is not recommended for HEPES-based PAGE systems.